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General Information
Symbol
Dmel\hpoMGH1
Species
D. melanogaster
Name
FlyBase ID
FBal0151847
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C19495185T

Reported nucleotide change:

C?T

Amino acid change:

Q503term | hpo-PA; Q503term | hpo-PB

Reported amino acid change:

Q504term

Comment:

A glutamine to stop mutation occuring in a polyglutamine stretch that is of varying lengths in different strains.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q504term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous clones generated using the eyFLP system result in eyes with an increased number of interommatidial cells compared to wild type.

Homozygous clones in the eye result in an increase in the overall size of the eye.

Eyes containing marked hpoMGH1 mutant and wild-type clones (generated throughout the eye disc using Scer\FLP1ey.PN with P{neoFRT}42D) have an over-representation of mutant tissue, suggesting that the mutant tissue has a relative growth advantage. Mutant ommatidial facets are slightly larger than wild-type facets and sometimes contain extra interommatidial bristles. The number of cells in hpoMGH1 mutant clones is consistently larger than in wild-type sister clones (clones generated at 48 hr after egg deposition (AED) and analyzed 72 hr later): The median population doubling time in these clones is 13.1 hr, which is significantly shorter than that for clone carrying a Avic\GFP bearing tester chromosome, which was 14.7 hr (P < 0.0001 by a Student's paired t test). Cell size in mutant clones is normal. Retinal sections reveal that ommatidia in hpoMGH1 mutant clones have the normal complement and arrangement of photoreceptor cells but are separated by significantly more interommatidial tissue. Additional layers interommatidial cells are apparent between ommatidia in mutant pupal retinas at 48 hours after puparium formation. At 38 hours APF, when excess interommatidial cells are eliminated by apoptosis, most of the cell death in discs containing hpoMGH1 mutant clones is restricted to wild-type portions of the disc. Homozygous clones of hpoMGH1 mutant cells in various imaginal discs, cause outgrowths of tissue. Portions of wings containing large hpoMGH1 clones are larger than the corresponding portion of a wild-type wing. In hpoMGH1 mosaic eye discs, the pattern of S phases is normal in the anterior portion of the disc and in the second mitotic wave (SMW) but in mutant portions these eye discs, S phase (BrdU-incorporating) nuclei can be seen posterior to the (SMW) and in the morphogenetic furrow (MF), a situation not seen in wild-type. i.e.- hpoMGH3 cells continue to replicate their DNA when surrounding wild-type cells are arrested in G1. Mitotic cells occur in mutant clones many ommatidial rows posterior to the SMW, a region devoid of mitoses in wild-type eye discs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressor of
Statement
Reference

hpoMGH1 is a suppressor | somatic clone of eye phenotype of grimGMR.PC

Additional Comments
Genetic Interactions
Statement
Reference

The increase in the number of interommatidial cells which is seen in homozygous hpoMGH1 clones generated using the eyFLP system is enhanced if the clones are also homozygous for either RtGEFp1036 or Gitex21C. No further enhancement over either double mutant phenotype is seen in the hpoMGH1, RtGEFp1036, Gitex21C triple mutant combination.

Homozygous hpoMGH1 clones in the eye result in an increase in the overall size of the eye. When the twin-spot is composed of Uba1A1 tissue, the overgrowth is enhanced.

The small, rough eye phenotye due to savGMR.PT; wtsGMR.PT, is suppressed when somatic clones of hpoMGH1 homozygous cells are generated throughout the eye discs using Scer\FLP1ey.PN with P{neoFRT}42D. The small, rough eye phenotype of grimGMR.PC flies is suppressed when somatic clones of hpoMGH1 homozygous cells are generated throughout the eye discs using Scer\FLP1ey.PN with P{neoFRT}42D. In the eye discs from these animals, the abnormal levels of cell death posterior to the morphogenetic furrow seen in grimGMR.PC eye discs are suppressed in the hpoMGH1 mutant tissue.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)