Allele Dmel\hpo^{K71R.Scer\UAS}

General Information
Symbol	Dmel\hpo^{K71R.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0151848
Feature type	allele	Associated gene	Dmel\hpo

Allele class
Mutagen	in vitro construct - site directed mutagenesis
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - site directed mutagenesis (Wu et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	hpo^Scer\UAS.cWa (Wu et al., 2003)
Nature of the lesion	Statement Reference Construct: Amino acid replacement: K71R. (Wu et al., 2003)
Carried in construct	P{UAS-hpo.K71R} (Wu et al., 2003, Mikeladze-Dvali et al., 2005, Jin et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^Bx-MS1096 (Jin et al., 2012)
Phenotype Manifest In
	photoreceptor cell R8 of pale ommatidium \| supernumerary, with Scer\GAL4^GMR.long (Mikeladze-Dvali et al., 2005) photoreceptor cell R8 of yellow ommatidium, with Scer\GAL4^GMR.long (Mikeladze-Dvali et al., 2005) wing, with Scer\GAL4^Bx-MS1096 (Wu et al., 2003, Jin et al., 2012)
Detailed Description
	Statement Reference Expression of hpo[K71R.Scer\UAS] under the control of Scer\GAL4[Bx-MS1096] results in a significant increase in wing size compared to controls. (Jin et al., 2012) When the kinase-dead hpo[K71R.Scer\UAS] transgene is expressed in eyes, under the control of Scer\GAL4[GMR.long], most R8 cells have a p fate, with only a few R8 cells adopting a y fate. (Mikeladze-Dvali et al., 2005) hpo^{K71R.Scer\UAS}; Scer\GAL4^Bx-MS1096 animals have enlarged wings (35% larger than wild-type). (Wu et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference hpo^{K71R.Scer\UAS}/Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, yki^Scer\UAS.cZa (Jin et al., 2012)
Phenotype Manifest In
Enhancer of
Statement Reference hpo^{K71R.Scer\UAS}/Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, yki^Scer\UAS.cZa (Jin et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of hpo[K71R.Scer\UAS] in flies expressing yki[Scer\UAS.cZa] under the control of Scer\GAL4[GMR.PU] results in a dramatic increase in eye size. (Jin et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	This mutation has been shown to kill the kinase activity of hpo protein. (Wu et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	hpo^{K71R.Scer\UAS} hpo^K71R (Wu et al., 2003)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Jin et al., 2012, J. Biol. Chem. 287(8): 5784--5796 Dimerization and cytoplasmic localization regulate Hippo kinase signaling activity in organ size control. [FBrf0217990] Mikeladze-Dvali et al., 2005, Cell 122(5): 775--787 The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors. [FBrf0187233] Wu et al., 2003, Cell 114(4): 445--456 hippo encodes a Ste-20 family protein kinase that restricts cell proliferation and promotes apoptosis in conjunction with salvador and warts. [FBrf0161987]

Allele Dmel\hpoK71R.Scer\UAS

Allele Dmel\hpo^{K71R.Scer\UAS}