Adult eyes containing hpoBF33
homozygous somatic clones (induced by the eyFLP method) display overgrowth and folding phenotype.
Homozygous clones in the eye disc result in overgrowth and adults have enlarged and folded eyes.
mutant eye disc clones exhibit tumorous overgrowth.
Homozygous clones in the eye result in enlarged and folded eyes.
Homozygous clones in the eye show progressive degeneration of photoreceptor cells.
mutant MARCM clones contain 5-7 cells per clone, compared to 2-3 cells in wild-type clones. These clones contain differentiated absorptive enterocytes and secretory enteroendocrine cells indicating that intestine stem cell differentiation continues as in wild-type. Phospho-His3
staining reveals an increase in the number of mitotic cells within the mutant clones. There is also an increase in the number of mitotic cells outside of the hpoBF33
mutant clones, indicating non-cell-autonomous proliferation.
Adults containing homozygous clones represent 36.4% of the population recovered after clones are induced using the eyFLP method (expected fraction of adults containing mutant clones is 50% if there is no effect of the mutant clones on viability).
mosaic eyes are significantly larger and often protrude out in folds. Tumourous outgrowths are also seen when clones are induced in other places, including the thorax wing and haltere. When mutant clones are made in the wing disc the size of the clones are significantly larger with more cells than the wild-type twin spots. The cell size is unaffected. More cells posterior to the second mitotic wave are seen to be in M phase than in wild-type.