FlyBase Allele Report: Dmel\foxo[UAS.cFa]

The presence of RU486 from day 2 of adulthood extends the median lifespan of female flies expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 on average by 6%.

The presence of RU486 from day 2 of adulthood extends the median lifespan of foxo^Δ94 female flies expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 on average by 10%.

Expressing foxo^Scer\UAS.cFa specifically in the mNSCs in the adult brain, using the Scer\GAL4^Ilp2.215-55 driver, extends the lifespan of female flies in both wild-type and foxo^Δ94 mutants.

Induction of foxo^Scer\UAS.cFa expression in the gut and fat body, under the control of Scer\GAL4^Switch1.106 enhances the climbing ability of female flies throughout their lifespan, observed as an increase in the proportion of high, or combined medium and high, climbers. This enhancement is seen in both wild-type and foxo^Δ94 backgrounds.

Induction of foxo^Scer\UAS.cFa expression in adulthood, under the control of Scer\GAL4^Switch1.106 (and RU486) has no effect on fecundity in either wild-type or foxo^Δ94 mutants, but has an effect on body weight and protein content.

(Alic et al., 2014)

Expression of foxo^Scer\UAS.cFa in the adult gut and fat body under the control of Scer\GAL4^Switch1.106 (in the presence of RU486) results in an extended lifespan compared to controls.

Expression of foxo^Scer\UAS.cFa specifically in the adult gut and under the control of Scer\GAL4^TIGS-2 (in the presence of RU486) has no major effect on lifespan compared to controls. Stored triacylglycerol (TAG) levels are also reduced compared to controls. Gut integrity and feeding appear normal.

(Alic et al., 2014)

Ectopic expression of foxo^Scer\UAS.cFa in the developing wing, under the control of Scer\GAL4^ptc-559.1 induces apoptosis and the subsequent loss of the wing anterior cross vein.

(Wang et al., 2014)

Motor neuronal overexpression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Rapgap1-OK6 does not result in neuromuscular junction overgrowth. These larvae display a modest reduction in the number of microtubule loops compared with wild-type.

(Nechipurenko and Broihier, 2012)

Female re-mating behaviour is not altered in flies expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 24 hours after mating to wild-type flies.

(Wigby et al., 2011)

Expression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Expression of foxo^Scer\UAS.cFa under the simultaneous control of both Scer\GAL4^Scer\UAS.cHa and Scer\GAL4^tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

(Birse et al., 2010)

Overexpression of foxo^Scer\UAS.cFa in muscles under the control of Scer\GAL4^Mhc.PW does not affect developmental growth and differentiation (as estimated by body weight and sarcomere assembly). Overexpression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Mhc.PW results in delayed accumulation of age-related protein aggregates in muscles.

Overexpression of foxo^Scer\UAS.cFa in adult muscles under the control of Scer\GAL4^Mef2.PR and Scer\GAL80^ts.αTub84B significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates. Compared to syngenic controls, the age-related accumulation of protein aggregates in retinas, brains and the peripheral fat body of the abdomen is also decreased in flies overexpressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Mhc.PW.

Muscle functionality gradually decreases in wild-type aging flies, resulting in impaired climbing and flight ability. Overexpression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Mhc.PW significantly preserves muscle strength during aging.

Overexpression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Mhc.PW in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

Food intake of flies is decreased in response to overexpression of foxo^Scer\UAS.cFa in muscles via Scer\GAL4^Mhc.PW.

The body weight of adult flies overexpressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Mhc.PW does not significantly differ from that of syngenic controls.

There is a significant decrease in the hemolymph glucose concentration compared with syngenic control in flies overexpressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

Expression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Ccap.PP results in the loss of adult-specific neurite projections; many central neurites and efferents are lost.

(Zhao et al., 2008)

Females expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 (in the presence of RU486) show an increase in mean and maximum lifespan compared with controls.

(Giannakou et al., 2005)

Females expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 (in the presence of RU486) show an increase in median life-span and reduced fecundity compared to controls. These females also show increased resistance to paraquat. No effect on life-span is seen in males expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 (in the presence of RU486).

(Giannakou et al., 2004)

When foxo^Scer\UAS.cFa is driven by Scer\GAL4^{bun-Switch1.32} (in adults fed on mifepristone) the median lifespan can increased by as much as 56%.

(Hwangbo et al., 2004)

In contrast to wild-type flies, the stress-induced heart failure rate of foxo^Scer\UAS.cFa, when under the control of both Scer\GAL4^tin.cBa and Scer\GAL4^Scer\UAS.cHa, does not increase with age.

(Wessells et al., 2004)

When expression is driven by Scer\GAL4^GMR.PF the eye is reduced and the ommatidial pattern disrupted.

(Junger et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^UAS.cFa has visible phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Junger et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, non-enhanceable by Su(var)205^HMS00278/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Suppressed by

Statement

Reference

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, suppressible by mor^HMS01267/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, suppressible by osa^HMS01738/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, suppressible by Iswi^HMS00628, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has abnormal adult locomotory behavior phenotype, suppressible by Iswi^HMS00628, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, suppressible by Etl1^HMS00829, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has chemical resistant phenotype, suppressible by mor^HMS01267/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has chemical resistant phenotype, suppressible by Iswi^HMS00628, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, suppressible by dom^HMS00142/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, suppressible by Tip60^HM05049/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, foxo^UAS.cFa has abnormal adult locomotory behavior phenotype, suppressible by mor^HMS01267/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Scer\GAL4^Act.PU, foxo^UAS.cFa has decreased cell size | somatic clone | larval stage phenotype, suppressible by Scer\GAL4^Act.PU/Xbp1^UAS.s.Tag:V5

(Zhao et al., 2021)

Scer\GAL4^Act.PU, foxo^UAS.cFa has decreased size | somatic clone | larval stage phenotype, suppressible by Scer\GAL4^Act.PU/Xbp1^UAS.s.Tag:V5

(Zhao et al., 2021)

Scer\GAL4^Cg.PA, foxo^UAS.cFa has decreased body size | larval stage phenotype, suppressible | partially by Xbp1^UAS.s.Tag:V5/Scer\GAL4^Cg.PA

(Zhao et al., 2021)

Scer\GAL4^Cg.PA, foxo^UAS.cFa has abnormal developmental rate phenotype, suppressible | partially by Xbp1^UAS.s.Tag:V5/Scer\GAL4^Cg.PA

(Zhao et al., 2021)

Scer\GAL4^Switch1.106, foxo^UAS.cFa, pnt^P1.UAS has short lived phenotype, suppressible by aop^ACT.UAS/Scer\GAL4^Switch1.106

(Alic et al., 2014)

Scer\GAL4^TIGS-2, foxo^UAS.cFa, pnt^P1.UAS has short lived phenotype, suppressible | partially by Scer\GAL4^TIGS-2/aop^ACT.UAS

(Alic et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^Switch1.106, foxo^UAS.cFa has long lived | female phenotype, non-suppressible by Su(var)205^HMS00278/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Enhancer of

Statement

Reference

foxo^UAS.cFa/Scer\GAL4^GMR58E02 is an enhancer of short lived | recessive phenotype of Fer2^MB09480

(Tas et al., 2018)

foxo^UAS.cFa/Scer\GAL4^Switch1.106 is an enhancer of short lived phenotype of Scer\GAL4^Switch1.106, pnt^P1.UAS

(Alic et al., 2014)

Scer\GAL4^TIGS-2/foxo^UAS.cFa is an enhancer of short lived phenotype of Scer\GAL4^TIGS-2, pnt^P1.UAS

(Alic et al., 2014)

NOT Enhancer of

Statement

Reference

foxo^UAS.cFa, Scer\GAL4^Switch1.106 is a non-enhancer of short lived phenotype of Mi-2^HMS00301, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Suppressor of

Statement

Reference

foxo^UAS.cFa/Scer\GAL4^Switch1.106 is a suppressor of short lived phenotype of Scer\GAL4^Switch1.106, dom^HMS00142

(Martínez Corrales et al., 2022)

foxo^UAS.cFa/Scer\GAL4^Switch1.106 is a suppressor of short lived phenotype of Scer\GAL4^Switch1.106, Tip60^HM05049

(Martínez Corrales et al., 2022)

foxo^UAS.cFa/Scer\GAL4^GMR58E02 is a suppressor | partially of decreased cell number | recessive | adult stage | progressive phenotype of Fer2^MB09480

(Tas et al., 2018)

foxo^UAS.cFa/Scer\GAL4^GMR58E02 is a suppressor | partially of abnormal locomotor behavior | recessive phenotype of Fer2^MB09480

(Tas et al., 2018)

Scer\GAL4^how-24B/foxo^UAS.cFa is a suppressor of abnormal locomotor behavior | recessive | adult stage phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^ple.PF/foxo^UAS.cFa is a suppressor of abnormal neuroanatomy | adult stage phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^how-24B/foxo^UAS.cFa is a suppressor of abnormal flight | recessive phenotype of park²⁵

(Tain et al., 2009)

NOT Suppressor of

Statement

Reference

foxo^UAS.cFa, Scer\GAL4^Switch1.106 is a non-suppressor of short lived phenotype of Mi-2^HMS00301, Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Other

Statement

Reference

Scer\GAL4^Switch1.106, foxo^UAS.cFa, nej^HMS01507 has short lived phenotype

(Martínez Corrales et al., 2022)

Scer\GAL4^Switch1.106, aop^HMS01256, foxo^UAS.cFa has short lived phenotype

(Alic et al., 2014)

Pink1^B9, Scer\GAL4^unspecified, foxo^UAS.cFa has lethal phenotype

(Tain et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^UAS.cFa has eye phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Junger et al., 2003)

Scer\GAL4^GMR.PF, foxo^UAS.cFa has ommatidium phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Junger et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, foxo^UAS.cFa has larval fat cell | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/Xbp1^UAS.s.Tag:V5

(Zhao et al., 2021)

Scer\GAL4^Act.PU, foxo^UAS.cFa has lipid droplet | somatic clone | larval stage phenotype, suppressible by Scer\GAL4^Act.PU/Xbp1^UAS.s.Tag:V5

(Zhao et al., 2021)

Suppressor of

Statement

Reference

foxo^UAS.cFa/Scer\GAL4^GMR58E02 is a suppressor | partially of adult dopaminergic PAM neuron | adult stage | progressive phenotype of Fer2^MB09480

(Tas et al., 2018)

Scer\GAL4^how-24B/foxo^UAS.cFa is a suppressor of indirect flight muscle cell phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^how-24B/foxo^UAS.cFa is a suppressor of mitochondrion phenotype of park²⁵

(Tain et al., 2009)

Scer\GAL4^ple.PF/foxo^UAS.cFa is a suppressor of dopaminergic PPL1 neuron phenotype of park²⁵

(Tain et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of foxo^UAS.cFa under the control of Scer\GAL4^GMR58E02 partially suppresses the progressive decrease in the number of adult PAM neurons and the decreased adult locomotion capacity observed in Fer2^MB09480 homozygotes, while further reducing their decreased lifespan.

(Tas et al., 2018)

Co-expression of foxo^Scer\UAS.cFa and aop^HMS01256 in the adult midgut and fat body under the control of Scer\GAL4^Switch1.106 (in the presence of RU486) results in a reduced lifespan compared to controls.

Expression of foxo^Scer\UAS.cFa enhances the reduction in lifespan seen when pnt^P1.Scer\UAS is expressed in the adult midgut and fat body under the control of Scer\GAL4^Switch1.106 (in the presence of RU486). This phenotype is completely suppressed upon expression of aop^ACT.Scer\UAS. Co-expression of foxo^Scer\UAS.cFa and pnt^P1.Scer\UAS also enhances the reduction in stored triacylglycerol (TAG) levels seen when either transgene is expressed alone. Gut integrity and feeding appear normal.

Expression of foxo^Scer\UAS.cFa enhances the reduction in lifespan seen when pnt^P1.Scer\UAS is expressed in the adult midgut under the control of Scer\GAL4^TIGS-2 (in the presence of RU486). This phenotype is partially suppressed upon expression of aop^ACT.Scer\UAS.

Flies co-expressing pnt^P1.Scer\UAS and foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 are starvation sensitive after 5 days in the presence of RU486, and this phenotype can be reversed upon expression of aop^ACT.Scer\UAS.

(Alic et al., 2014)

Expression of foxo^Scer\UAS.cFa driven by Scer\GAL4^how-24B significantly partially suppresses flight and climbing defects as well as muscle degeneration and mitochondrial disruption in park²⁵/park²⁵ flies; foxo^Scer\UAS.cFa driven by Scer\GAL4^ple.PF significantly suppresses loss of dopaminergic PPL1 neurons in 30 day old park²⁵/park²⁵ flies. Expression of foxo^Scer\UAS.cFa in a Pink1^B9/Pink1^B9 background results in lethality.

(Tain et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

foxo^UAS.cFa/Scer\GAL4^Lk6-DJ634 rescues foxo²⁵/foxo²¹

(Zheng et al., 2007)

Partially rescues

foxo^UAS.cFa/Scer\GAL4^GMR58E02 partially rescues foxo^Δ94

(Tas et al., 2018)

foxo^UAS.cFa/Scer\GAL4^Switch1.106 partially rescues foxo^Δ94

(Alic et al., 2014)

Scer\GAL4^Ilp2.215-55/foxo^UAS.cFa partially rescues foxo^Δ94

(Alic et al., 2014)

Fails to rescue

foxo^UAS.cFa/Scer\GAL4^P2.4.Pdf fails to rescue foxo²⁵/foxo²¹

(Zheng et al., 2007)

Comments

The expression of foxo^UAS.cFa under the control of Scer\GAL4^GMR58E02 rescues the decreased number of dopaminergic PAM neurons in the adult brain but enhances the decreased lifespan presented by foxo^Δ94 homozygotes.

(Tas et al., 2018)

The presence of RU486 from day 2 of adulthood extends the median lifespan of foxo^Δ94 female flies expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 on average by 10%.

Expressing foxo^Scer\UAS.cFa specifically in the mNSCs in the adult brain, using the Scer\GAL4^Ilp2.215-55 driver, extends the lifespan of female flies in foxo^Δ94 mutants.

Induction of foxo^Scer\UAS.cFa expression in adulthood, under the control of Scer\GAL4^Switch1.106 (and RU486) has no effect on fecundity in foxo^Δ94 mutants, but has an effect on body weight and protein content.

(Alic et al., 2014)

Expression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Lk6-DJ634 rescues the circadian arrhythmia seen in foxo²¹/foxo²⁵ mutants. Phototaxis is not affected by paraquat treatment in these mutants.

(Zheng et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

80946

y¹ w^*; P{UAS-foxo.F}3

80949

y¹ w^*; P{UAS-foxo.F}3, foxo²¹/TM3, Sb¹

Notes on Origin

Discoverer