Allele Dmel\foxo^Scer\UAS.cFa

General Information
Symbol	Dmel\foxo^Scer\UAS.cFa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Junger	FlyBase ID	FBal0151927
Feature type	allele	Associated gene	Dmel\foxo

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Junger et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of cDNA for foxo is governed by Scer\UAS regulatory sequences. (Junger et al., 2003)
Carried in construct	P{UAS-foxo.F} (Tatar, 2005, Giannakou et al., 2005, Giannakou et al., 2004, Hwangbo et al., 2004, Wessells et al., 2004, Junger et al., 2003, Zheng et al., 2007, Zhao et al., 2008, Birse et al., 2010, Wigby et al., 2011, Demontis and Perrimon, 2010, Nechipurenko and Broihier, 2012)
Cytology
Phenotypic Data
Phenotypic Class
	aging defective, with Scer\GAL4^Mef2.PR, Scer\GAL80^ts.αTub84B (Demontis and Perrimon, 2010) aging defective, with Scer\GAL4^Mhc.PW (Demontis and Perrimon, 2010) aging defective, with Scer\GAL4^Scer\UAS.cHa, Scer\GAL4^tin.cBa (Wessells et al., 2004) chemical resistant \| RU486 conditional, with Scer\GAL4^Switch1.106 (Giannakou et al., 2004) feeding behavior defective, with Scer\GAL4^Mhc.PW (Demontis and Perrimon, 2010) female fertile \| reduced \| RU486 conditional, with Scer\GAL4^Switch1.106 (Giannakou et al., 2004) lethal \| larval stage, with Scer\GAL4^Act.PU (Hwangbo et al., 2004) long lived, with Scer\GAL4^Mhc.PW (Demontis and Perrimon, 2010) long lived \| female \| RU486 conditional, with Scer\GAL4^Switch1.106 (Giannakou et al., 2004, Giannakou et al., 2005) neuroanatomy defective \| pharate adult stage, with Scer\GAL4^Ccap.PP (Zhao et al., 2008) viable, with Scer\GAL4^Scer\UAS.cHa, Scer\GAL4^tin.cBa (Wessells et al., 2004) visible, with Scer\GAL4^GMR.PF (Junger et al., 2003) wild-type \| RU486 conditional, with Scer\GAL4^Switch1.106 (Hwangbo et al., 2004)
Phenotype Manifest In
	abdominal adult fat mass, with Scer\GAL4^Mhc.PW (Demontis and Perrimon, 2010) adult brain, with Scer\GAL4^Mhc.PW (Demontis and Perrimon, 2010) adult heart, with Scer\GAL4^Scer\UAS.cHa, Scer\GAL4^tin.cBa (Wessells et al., 2004) eye, with Scer\GAL4^GMR.PF (Junger et al., 2003) microtubule, with Scer\GAL4^Rapgap1-OK6 (Nechipurenko and Broihier, 2012) muscle cell, with Scer\GAL4^Mef2.PR, Scer\GAL80^ts.αTub84B (Demontis and Perrimon, 2010) muscle cell, with Scer\GAL4^Mhc.PW (Demontis and Perrimon, 2010) neurite \| pharate adult stage, with Scer\GAL4^Ccap.PP (Zhao et al., 2008) ommatidium, with Scer\GAL4^GMR.PF (Junger et al., 2003) retina, with Scer\GAL4^Mhc.PW (Demontis and Perrimon, 2010)
Detailed Description
	Statement Reference Motor neuronal overexpression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Rapgap1-OK6] does not result in neuromuscular junction overgrowth. These larvae display a modest reduction in the number of microtubule loops compared with wild-type. (Nechipurenko and Broihier, 2012) Female re-mating behaviour is not altered in flies expressing foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Switch1.106] 24 hours after mating to wild-type flies. (Wigby et al., 2011) Expression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Lsp2.PH] rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet. Expression of foxo[Scer\UAS.cFa] under the simultaneous control of both Scer\GAL4[Scer\UAS.cHa] and Scer\GAL4[tin.cBa] does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals. (Birse et al., 2010) Overexpression of foxo[Scer\UAS.cFa] in muscles under the control of Scer\GAL4[Mhc.PW] does not affect developmental growth and differentiation (as estimated by body weight and sarcomere assembly). Overexpression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Mhc.PW] results in delayed accumulation of age-related protein aggregates in muscles. Overexpression of foxo[Scer\UAS.cFa] in adult muscles under the control of Scer\GAL4[Mef2.PR] and Scer\GAL80[ts.αTub84B] significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates. Compared to syngenic controls, the age-related accumulation of protein aggregates in retinas, brains and the peripheral fat body of the abdomen is also decreased in flies overexpressing foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Mhc.PW]. Muscle functionality gradually decreases in wild-type aging flies, resulting in impaired climbing and flight ability. Overexpression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Mhc.PW] significantly preserves muscle strength during aging. Overexpression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Mhc.PW] in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies. Food intake of flies is decreased in response to overexpression of foxo[Scer\UAS.cFa] in muscles via Scer\GAL4[Mhc.PW]. The body weight of adult flies overexpressing foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Mhc.PW] does not significantly differ from that of syngenic controls. There is a significant decrease in the hemolymph glucose concentration compared with syngenic control in flies overexpressing foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Mhc.PW] (Demontis and Perrimon, 2010) Expression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Ccap.PP] results in the loss of adult-specific neurite projections; many central neurites and efferents are lost. (Zhao et al., 2008) Females expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 (in the presence of RU486) show an increase in mean and maximum lifespan compared with controls. (Giannakou et al., 2005) Females expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 (in the presence of RU486) show an increase in median life-span and reduced fecundity compared to controls. These females also show increased resistance to paraquat. No effect on life-span is seen in males expressing foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 (in the presence of RU486). (Giannakou et al., 2004) When foxo^Scer\UAS.cFa is driven by Scer\GAL4^{bun-Switch1.32} (in adults fed on mifepristone) the median lifespan can increased by as much as 56%. (Hwangbo et al., 2004) In contrast to wild-type flies, the stress-induced heart failure rate of foxo^Scer\UAS.cFa, when under the control of both Scer\GAL4^tin.cBa and Scer\GAL4^Scer\UAS.cHa, does not increase with age. (Wessells et al., 2004) When expression is driven by Scer\GAL4^GMR.PF the eye is reduced and the ommatidial pattern disrupted. (Junger et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, foxo^Scer\UAS.cFa has visible phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Junger et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, foxo^Scer\UAS.cFa has eye phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Junger et al., 2003) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, foxo^Scer\UAS.cFa has ommatidium phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Junger et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	foxo^Scer\UAS.cFa/Scer\GAL4^Lk6-DJ634 rescues foxo²⁵/foxo²¹ (Zheng et al., 2007)
Fails to rescue	foxo^Scer\UAS.cFa/Scer\GAL4^P2.4.Pdf fails to rescue foxo²⁵/foxo²¹ (Zheng et al., 2007)
Comments	Expression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Lk6-DJ634] rescues the circadian arrhythmia seen in foxo[21]/foxo[25] mutants. Phototaxis is not affected by paraquat treatment in these mutants. (Zheng et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	foxo^Scer\UAS.cFa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Junger (Junger et al., 2003)
Secondary FlyBase IDs

References ( 12 )
Research paper	Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] Wigby et al., 2011, Proc. Biol. Sci. 278(1704): 424--431 Insulin signalling regulates remating in female Drosophila. [FBrf0212666] Birse et al., 2010, Cell Metab. 12(5): 533--544 High-fat-diet-induced obesity and heart dysfunction are regulated by the TOR pathway in Drosophila. [FBrf0212220] Demontis and Perrimon, 2010, Cell 143(5): 813--825 FOXO/4E-BP Signaling in Drosophila Muscles Regulates Organism-wide Proteostasis during Aging. [FBrf0212347] Zhao et al., 2008, Genetics 178(2): 883--901 A Drosophila gain-of-function screen for candidate genes involved in steroid-dependent neuroendocrine cell remodeling. [FBrf0204350] Zheng et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(40): 15899--15904 FOXO and insulin signaling regulate sensitivity of the circadian clock to oxidative stress. [FBrf0216135] Giannakou et al., 2004, Science 305(5682): 361 Long-lived Drosophila with overexpressed dFOXO in adult fat body. [FBrf0179222] Hwangbo et al., 2004, Nature 429(6991): 562--566 Drosophila dFOXO controls lifespan and regulates insulin signalling in brain and fat body. [FBrf0179031] Wessells et al., 2004, Nat. Genet. 36(12): 1275--1281 Insulin regulation of heart function in aging fruit flies. [FBrf0184189] Junger et al., 2003, J. Biol. 2(3): 20 The Drosophila Forkhead transcription factor FOXO mediates the reduction in cell number associated with reduced insulin signaling. [FBrf0162177]
Note	Giannakou et al., 2005, Science 307(5710): 675 Response to comment on 'Long-lived Drosophila with overexpressed dFOXO in adult fat body'. [FBrf0188461] Tatar, 2005, Science 307(5710): 675 Comment on 'Long-lived Drosophila with overexpressed dFOXO in adult fat body'. [FBrf0188462]

Allele Dmel\foxoScer\UAS.cFa

Allele Dmel\foxo^Scer\UAS.cFa