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General Information
Symbol
Dmel\foxoUAS.cFa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Junger
FlyBase ID
FBal0151927
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dFOXO, UAS-foxo
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of cDNA for foxo is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The presence of RU486 from day 2 of adulthood extends the median lifespan of female flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 on average by 6%.

The presence of RU486 from day 2 of adulthood extends the median lifespan of foxoΔ94 female flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 on average by 10%.

Expressing foxoScer\UAS.cFa specifically in the mNSCs in the adult brain, using the Scer\GAL4Ilp2.215-55 driver, extends the lifespan of female flies in both wild-type and foxoΔ94 mutants.

Induction of foxoScer\UAS.cFa expression in the gut and fat body, under the control of Scer\GAL4Switch1.106 enhances the climbing ability of female flies throughout their lifespan, observed as an increase in the proportion of high, or combined medium and high, climbers. This enhancement is seen in both wild-type and foxoΔ94 backgrounds.

Induction of foxoScer\UAS.cFa expression in adulthood, under the control of Scer\GAL4Switch1.106 (and RU486) has no effect on fecundity in either wild-type or foxoΔ94 mutants, but has an effect on body weight and protein content.

Expression of foxoScer\UAS.cFa in the adult gut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486) results in an extended lifespan compared to controls.

Expression of foxoScer\UAS.cFa specifically in the adult gut and under the control of Scer\GAL4TIGS-2 (in the presence of RU486) has no major effect on lifespan compared to controls. Stored triacylglycerol (TAG) levels are also reduced compared to controls. Gut integrity and feeding appear normal.

Ectopic expression of foxoScer\UAS.cFa in the developing wing, under the control of Scer\GAL4ptc-559.1 induces apoptosis and the subsequent loss of the wing anterior cross vein.

Motor neuronal overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Rapgap1-OK6 does not result in neuromuscular junction overgrowth. These larvae display a modest reduction in the number of microtubule loops compared with wild-type.

Female re-mating behaviour is not altered in flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 24 hours after mating to wild-type flies.

Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Expression of foxoScer\UAS.cFa under the simultaneous control of both Scer\GAL4Scer\UAS.cHa and Scer\GAL4tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

Overexpression of foxoScer\UAS.cFa in muscles under the control of Scer\GAL4Mhc.PW does not affect developmental growth and differentiation (as estimated by body weight and sarcomere assembly). Overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW results in delayed accumulation of age-related protein aggregates in muscles.

Overexpression of foxoScer\UAS.cFa in adult muscles under the control of Scer\GAL4Mef2.PR and Scer\GAL80ts.αTub84B significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates. Compared to syngenic controls, the age-related accumulation of protein aggregates in retinas, brains and the peripheral fat body of the abdomen is also decreased in flies overexpressing foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW.

Muscle functionality gradually decreases in wild-type aging flies, resulting in impaired climbing and flight ability. Overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW significantly preserves muscle strength during aging.

Overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

Food intake of flies is decreased in response to overexpression of foxoScer\UAS.cFa in muscles via Scer\GAL4Mhc.PW.

The body weight of adult flies overexpressing foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW does not significantly differ from that of syngenic controls.

There is a significant decrease in the hemolymph glucose concentration compared with syngenic control in flies overexpressing foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW

Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Ccap.PP results in the loss of adult-specific neurite projections; many central neurites and efferents are lost.

Females expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 (in the presence of RU486) show an increase in mean and maximum lifespan compared with controls.

Females expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 (in the presence of RU486) show an increase in median life-span and reduced fecundity compared to controls. These females also show increased resistance to paraquat. No effect on life-span is seen in males expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 (in the presence of RU486).

When foxoScer\UAS.cFa is driven by Scer\GAL4bun-Switch1.32 (in adults fed on mifepristone) the median lifespan can increased by as much as 56%.

In contrast to wild-type flies, the stress-induced heart failure rate of foxoScer\UAS.cFa, when under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa, does not increase with age.

When expression is driven by Scer\GAL4GMR.PF the eye is reduced and the ommatidial pattern disrupted.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The expression of foxoUAS.cFa under the control of Scer\GAL4GMR58E02 partially suppresses the progressive decrease in the number of adult PAM neurons and the decreased adult locomotion capacity observed in Fer2MB09480 homozygotes, while further reducing their decreased lifespan.

Co-expression of foxoScer\UAS.cFa and aopHMS01256 in the adult midgut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486) results in a reduced lifespan compared to controls.

Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486). This phenotype is completely suppressed upon expression of aopACT.Scer\UAS. Co-expression of foxoScer\UAS.cFa and pntP1.Scer\UAS also enhances the reduction in stored triacylglycerol (TAG) levels seen when either transgene is expressed alone. Gut integrity and feeding appear normal.

Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut under the control of Scer\GAL4TIGS-2 (in the presence of RU486). This phenotype is partially suppressed upon expression of aopACT.Scer\UAS.

Flies co-expressing pntP1.Scer\UAS and foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 are starvation sensitive after 5 days in the presence of RU486, and this phenotype can be reversed upon expression of aopACT.Scer\UAS.

Expression of foxoScer\UAS.cFa driven by Scer\GAL4how-24B significantly partially suppresses flight and climbing defects as well as muscle degeneration and mitochondrial disruption in park25/park25 flies; foxoScer\UAS.cFa driven by Scer\GAL4ple.PF significantly suppresses loss of dopaminergic PPL1 neurons in 30 day old park25/park25 flies. Expression of foxoScer\UAS.cFa in a Pink1B9/Pink1B9 background results in lethality.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The expression of foxoUAS.cFa under the control of Scer\GAL4GMR58E02 rescues the decreased number of dopaminergic PAM neurons in the adult brain but enhances the decreased lifespan presented by foxoΔ94 homozygotes.

The presence of RU486 from day 2 of adulthood extends the median lifespan of foxoΔ94 female flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 on average by 10%.

Expressing foxoScer\UAS.cFa specifically in the mNSCs in the adult brain, using the Scer\GAL4Ilp2.215-55 driver, extends the lifespan of female flies in foxoΔ94 mutants.

Induction of foxoScer\UAS.cFa expression in the gut and fat body, under the control of Scer\GAL4Switch1.106 enhances the climbing ability of female flies throughout their lifespan, observed as an increase in the proportion of high, or combined medium and high, climbers. This enhancement is seen in a foxoΔ94 background.

Induction of foxoScer\UAS.cFa expression in adulthood, under the control of Scer\GAL4Switch1.106 (and RU486) has no effect on fecundity in foxoΔ94 mutants, but has an effect on body weight and protein content.

Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Lk6-DJ634 rescues the circadian arrhythmia seen in foxo21/foxo25 mutants. Phototaxis is not affected by paraquat treatment in these mutants.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
foxoScer\UAS.cFa
foxoUAS.cFa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Junger
Secondary FlyBase IDs
    References (23)