Allele Dmel\foxo²⁵

General Information
Symbol	Dmel\foxo²⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0151929
Feature type	allele	Associated gene	Dmel\foxo
Also Known As	dFOXO²⁵

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Dionne et al., 2006)
Mutagen	P-element activity (Junger et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:9,892,810..9,892,810 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=W124\|foxo-PC,W124\|foxo-PA,W124@\|foxo-PB reported_pr_change=W124@ na_change=G9892810A (Junger et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	foxo^EP35-147 (Junger et al., 2003)
Nature of the lesion	Statement Reference Amino acid replacement: W124@. (Junger et al., 2003)
Caused by insertion	P{EP}foxo^EP35-147 (Junger et al., 2003, Tettweiler et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	aging defective (with foxo²¹) (Demontis and Perrimon, 2010) circadian rhythm defective (with foxo²¹) (Zheng et al., 2007) developmental rate defective (with foxo^Δ94) (Slack et al., 2011) developmental rate defective \| nutrition conditional (with foxo²¹) (Kramer et al., 2008) immune response defective (with foxo^BG01018) (Dionne et al., 2006) oxidative stress response defective (with foxo²¹) (Zheng et al., 2007) short lived (with foxo²¹) (Sun et al., 2012) short lived \| nutrition conditional (with foxo²¹) (Kramer et al., 2008) small body (with foxo²¹) (Kramer et al., 2008) small body (with foxo^Δ94) (Slack et al., 2011) stress response defective (Junger et al., 2003) stress response defective (with foxo²¹) (Tettweiler et al., 2005) viable (Junger et al., 2003, Liu and Lehmann, 2006)
Phenotype Manifest In
	muscle cell (with foxo²¹) (Demontis and Perrimon, 2010) mushroom body neuroblast (Siegrist et al., 2010) wing (Junger et al., 2003) wing (with foxo^Δ94) (Slack et al., 2011)
Detailed Description
	Statement Reference foxo[21]/foxo[25] flies are short lived relative to wild-type controls, but their lifespan patterns responding to diet are similar to their wild-type controls. (Sun et al., 2012) foxo[21]/foxo[25] mutant animals display accelerated muscle aging manifesting in the increased accumulation of age-related protein aggregates compared with wild-type flies. (Demontis and Perrimon, 2010) Mushroom body neuroblasts persist slightly longer than normal in mutant animals. (Siegrist et al., 2010) foxo[21]/foxo[25] transheterozygous larvae exhibit reduced survival and shorter life span under amino acid starvation conditions. These flies are also developmentally delayed under these conditions and are smaller than wild-type after 48 hours. (Kramer et al., 2008) foxo[25]/foxo[21] trans-heterozygotes exhibit normal lipid levels and are as resistant to starvation as wild-type flies. (Wang et al., 2008) Starvation-induced autophagy is strongly reduced compared to controls in foxo[21]/foxo[25] larvae. (Juhász et al., 2007) foxo[21]/foxo[25] mutants rapidly become arrythmic in the presence of low levels of paraquat (0.5-1mM), while wild-type flies retain their rhythms for a few weeks. Phototaxis is not affected by paraquat treatment in these mutants. foxo[21]/foxo[25] mutant flies are hypersensitive to oxidative stress. 30 day old foxo[21]/foxo[25] mutant flies show weak rhythms simliar to those seen in response to paraquat in young flies. (Zheng et al., 2007) foxo^BG01018/foxo²⁵ flies display a 4.8% improvement in medial survival following M.marinum infection in comparison to wild-type flies - 174 hours vs. 166 hours. foxo²¹/foxo²⁵ and foxo²⁵/Df(3R)ED5634 transheterozygotes survive even longer - 190 hours following infection. The number of bacteria recorded in these flies is not significantly different from wild-type flies. (Dionne et al., 2006) Salivary glands are destroyed at the same time in homozygous pupae as in wild-type pupae. (Liu and Lehmann, 2006) foxo²¹/foxo²⁵ flies exhibit a reduced life-span when exposed to 5% hydrogen peroxide-containing media (median life-span of 34.5 hours). Only 2% of foxo²¹/foxo²⁵ flies survived 60 hours after exposure to oxidative stress, compared to 66.1% in wild-type. (Tettweiler et al., 2005) Shows no obvious phenotype under normal culturing conditions, though close inspection reveals the wing size is slightly reduced. Clonal analysis in the head capsule reveals no effect on growth. Clonal analysis reveals no difference of cell size in the developing eye between mutant and wild type. No significant difference between body weight of mutant and wild type flies is detectable. When placed on hydrogen-peroxide-containing food mutant flies display significantly reduced survival time compared to control flies. A similar effect occurs in response to paraquat feeding. (Junger et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference foxo²⁵/foxo²¹ has stress response defective phenotype, suppressible by Scer\GAL4^Act5C.PU/Thor^Scer\UAS.cTa (Tettweiler et al., 2005)
Enhancer of
Statement Reference foxo²⁵/foxo²¹ is an enhancer of decreased cell size phenotype of chico^flp147E/chico¹ (Junger et al., 2003) foxo²⁵ is an enhancer of visible \| somatic clone phenotype of Tsc1^Q87X (Junger et al., 2003)
NOT Enhancer of
Statement Reference foxo²⁵/foxo[+] is a non-enhancer of short lived phenotype of Crtc^25-3 (Wang et al., 2008) foxo²⁵/foxo[+] is a non-enhancer of stress response defective phenotype of Crtc^25-3 (Wang et al., 2008)
Suppressor of
Statement Reference foxo²⁵/foxo[+] is a suppressor \| partially of small body \| adult stage phenotype of Scer\GAL4^Ilp2.PR, hep^Act.Scer\UAS (Wang et al., 2005) foxo²⁵/foxo[+] is a suppressor of long lived \| dominant phenotype of puc^E69 (Wang et al., 2005) foxo²⁵/foxo²¹ is a suppressor \| partially of lethal \| larval stage phenotype of Akt1¹ (Junger et al., 2003) foxo²⁵/foxo²¹ is a suppressor \| partially of small body phenotype of chico^flp147E/chico¹ (Junger et al., 2003) foxo²⁵/foxo²¹ is a suppressor of decreased cell number phenotype of chico^flp147E/chico¹ (Junger et al., 2003) foxo²⁵/foxo²¹ is a suppressor of visible phenotype of chico^flp147E/chico¹ (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of visible \| somatic clone phenotype of Akt1¹ (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of visible \| somatic clone phenotype of InR³⁰⁴ (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of visible \| somatic clone phenotype of Pi3K92E^5W3 (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of visible \| somatic clone phenotype of Pten¹¹⁷ (Junger et al., 2003)
NOT Suppressor of
Statement Reference foxo²⁵/foxo[+] is a non-suppressor of decreased cell death phenotype of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB (Liu and Lehmann, 2006) foxo²⁵/foxo[+] is a non-suppressor of short lived phenotype of Crtc^25-3 (Wang et al., 2008) foxo²⁵/foxo[+] is a non-suppressor of stress response defective phenotype of Crtc^25-3 (Wang et al., 2008) foxo²⁵/foxo²⁵ is a non-suppressor of decreased cell death phenotype of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB (Liu and Lehmann, 2006, Liu and Lehmann, 2006)
Other
Statement Reference Akt1¹, foxo²⁵ has lethal phenotype (Liu and Lehmann, 2006) Crtc^25-3, foxo²¹, foxo²⁵ has lethal phenotype (Wang et al., 2008)
Phenotype Manifest In
Enhancer of
Statement Reference foxo²⁵/foxo²¹ is an enhancer of ommatidium phenotype of chico^flp147E/chico¹ (Junger et al., 2003) foxo²⁵ is an enhancer of head capsule \| somatic clone phenotype of Tsc1^Q87X (Junger et al., 2003)
Suppressor of
Statement Reference foxo²⁵/foxo²¹ is a suppressor of ommatidium phenotype of chico^flp147E/chico¹ (Junger et al., 2003) foxo²⁵/foxo²¹ is a suppressor of wing phenotype of chico^flp147E/chico¹ (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of head capsule \| somatic clone phenotype of Akt1¹ (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of head capsule \| somatic clone phenotype of InR³⁰⁴ (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of head capsule \| somatic clone phenotype of Pi3K92E^5W3 (Junger et al., 2003) foxo²⁵ is a suppressor \| partially of head capsule \| somatic clone phenotype of Pten¹¹⁷ (Junger et al., 2003)
NOT Suppressor of
Statement Reference foxo²⁵/foxo[+] is a non-suppressor of embryonic/larval salivary gland phenotype of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB (Liu and Lehmann, 2006) foxo²⁵/foxo²¹ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, Crtc^Scer\UAS.cWa (Wang et al., 2008) foxo²⁵/foxo²⁵ is a non-suppressor of embryonic/larval salivary gland phenotype of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB (Liu and Lehmann, 2006, Liu and Lehmann, 2006)
Other
Statement Reference Akt1¹, foxo²⁵ has embryonic/larval salivary gland phenotype (Liu and Lehmann, 2006) Scer\GAL4^wor.PA, W^{miRNA.RHG.Scer\UAS}, foxo²⁵, grim^{miRNA.RHG.Scer\UAS}, rpr^{miRNA.RHG.Scer\UAS} has adult mushroom body phenotype (Siegrist et al., 2010) Scer\GAL4^wor.PA, W^{miRNA.RHG.Scer\UAS}, foxo²⁵, grim^{miRNA.RHG.Scer\UAS}, rpr^{miRNA.RHG.Scer\UAS} has neuroblast \| ectopic phenotype (Siegrist et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Lipid levels are completely restored in Sik3[48] ; foxo[21]/foxo[25] double mutant flies. (Wang et al., 2011) Neuroblasts are detected in the mushroom body of 2 week old and even in 1 month old adults simultaneously co-expressing rpr[miRNA.RHG.Scer\UAS], W[miRNA.RHG.Scer\UAS] and grim[miRNA.RHG.Scer\UAS] (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4[wor.PA] in a foxo[25] background (mushroom body neuroblasts are not seen in wild-type adults and are not seen at these late time points in adults expressing the P{UAS-RHG.miRNA} transgene under the control of Scer\GAL4[wor.PA] in a wild-type background or in foxo[25] single mutant adults). Some of the 2 week old mutant mushroom body neuroblasts are large in size and generate many new progeny. There is a strong correlation between mushroom body neuroblast cell size and progeny number in 2 week old mutant adults, but not in younger mutant adults. In 1 month old mutant adults, some of the progeny of these neuroblasts show normal axon projections through the mushroom body pedunculus and some mistarget, bifurcating prematurely and projecting anterior to the pedunculus. (Siegrist et al., 2010) A foxo[25] heterozygous background has no effect on survival or lipid accumulation in starved TORC[25-3] homozygotes. Removal of both copies of foxo (foxo[25]/foxo[21]) is lethal in TORC[25-3] homozygotes. TORC[Scer\UAS.cWa] over-expressing flies (driven by Scer\GAL4[GMR.PF]) in which foxo is reduced or eliminated though a foxo[25]/foxo[21] background still exhibit a rough eye phenotype. (Wang et al., 2008) foxo²⁵ Akt1¹ double homozygotes show considerable lethality; most animals die before the pupal stage, with most of the remaining animals dying as pupae and only a few escapers eclosing as adults. 71% of pupae still contain intact salivary glands at 20 hours after puparium formation (this is approximately 6 hours after the glands are normally destroyed in wild-type animals). (Liu and Lehmann, 2006) Ectopic expression of Thor^Scer\UAS.cTa, under the control of Scer\GAL4^Act5C can completely suppress the sensitivity of foxo²¹/foxo²⁵ flies to oxidative stress (median life-span of 56.8 hours, 39.7% survival rate after 60 hours exposure to 5% hydrogen peroxide). (Tettweiler et al., 2005) The increase in average and maximum lifespan seen in puc^E69/+ adults is dominantly suppressed by foxo²⁵. The reduction in body weight seen in 1 day old hep^Act.Scer\UAS; Scer\GAL4^Ilp2.PR adults is partially supressed by foxo²⁵/+. (Wang et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	foxo²⁵/foxo²¹ is rescued by foxo^Scer\UAS.cFa/Scer\GAL4^Lk6-DJ634 (Zheng et al., 2007) foxo²⁵/foxo²¹ is rescued by foxo^T:SV5\V5 (Zheng et al., 2007) foxo²⁵/foxo²¹ is rescued by Scer\GAL4^Lk6-DJ634/foxo^{+t4.6.Scer\UAS.T:SV5\V5} (Zheng et al., 2007)
Not rescued by	foxo²⁵/foxo²¹ is not rescued by foxo^{+t4.6.Scer\UAS.T:SV5\V5}/Scer\GAL4^P2.4.Pdf (Zheng et al., 2007) foxo²⁵/foxo²¹ is not rescued by foxo^Scer\UAS.cFa/Scer\GAL4^P2.4.Pdf (Zheng et al., 2007) foxo²⁵/foxo²¹ is not rescued by foxo^T:SV5\V5 (Zheng et al., 2007)
Comments	Expression of foxo[T:SV5\V5] rescues the circadian arrhythmia seen in foxo[21]/foxo[25] mutants. Phototaxis is not affected by paraquat treatment in these mutants. Expression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Lk6-DJ634] rescues the circadian arrhythmia seen in foxo[21]/foxo[25] mutants. Phototaxis is not affected by paraquat treatment in these mutants. (Zheng et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	25 (Demontis and Perrimon, 2010) dfoxo25c (Slack et al., 2010) dFOXO²⁵ (Puig and Tjian, 2005, Junger et al., 2003, Liu and Lehmann, 2006, Vihervaara and Puig, 2008, Chung et al., 2010) dfoxo²⁵ (Buch et al., 2008, Sun et al., 2012) dFoxO²⁵ (Kanao et al., 2010) dFoxo^Rw25 (Slaidina et al., 2009) foxo25 (Zid et al., 2009) FOXO²⁵ (Teleman et al., 2005, Wang et al., 2011, Francis et al., 2010) foxo²⁵ (Dionne et al., 2006, Nechipurenko and Broihier, 2012, Wang et al., 2008, Xu et al., 2012, Siegrist et al., 2010, Kockel et al., 2010, Kramer et al., 2008, Zheng et al., 2007, Slack et al., 2011) Foxo²⁵ (Juhász et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 28 )

Generate a list of	All references relating to this allele ( 28 )

List References by type	Research paper ( 28 )
Recent research papers ( 5 )
	Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] Sun et al., 2012, Aging Cell 11(5): 783--793 Nutrient-dependent requirement for SOD1 in lifespan extension by protein restriction in Drosophila melanogaster. [FBrf0219634] Xu et al., 2012, PLoS Genet. 8(1): e1002478 Insulin Signaling Regulates Fatty Acid Catabolism at the Level of CoA Activation. [FBrf0217254] Slack et al., 2011, Aging Cell 10(5): 735--748 dFOXO-independent effects of reduced insulin-like signaling in Drosophila. [FBrf0215229] Wang et al., 2011, Cell 145(4): 596--606 A hormone-dependent module regulating energy balance. [FBrf0213699] Show all research papers ...

Allele Dmel\foxo25

Allele Dmel\foxo²⁵