FlyBase Allele Report: Dmel\foxo[21]

General Information

Symbol

Dmel\foxo²¹

Species

D. melanogaster

Name

FlyBase ID

FBal0151930

Feature type

allele

Associated gene

Dmel\foxo

Associated Insertion(s)

P{EP}EP35-147

Carried in Construct

Also Known As

dFOXO²¹

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Dionne et al., 2006)

Mutagen

P-element activity

(Junger et al., 2003)

Progenitor genotype

foxo^EP35-147

(Junger et al., 2003)

Associated Insertion(s)

P{EP}EP35-147

Cytology

Description

The progenitor P{EP}EP35-147 insertion is also still present on the chromosome.

(Junger et al., 2003)

Amino acid replacement: W95term.

(Junger et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:14,067,001..14,067,001 [+]

Nucleotide change:

G14067001A

Amino acid change:

Reported amino acid change:

W95term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Junger et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Hong et al., 2012)

ameliorates lower respiratory tract disease

modeled by PGRP-LC^x.UAS

(Wagner et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm (with foxo²⁵)

(Zheng et al., 2007)

abnormal developmental rate | nutrition conditional (with foxo²⁵)

(Kramer et al., 2008)

abnormal immune response (with foxo^BG01018)

(Dionne et al., 2006)

abnormal locomotor behavior | adult stage | progressive (with foxo²⁵)

(Koh et al., 2012)

abnormal oxidative stress response (with foxo²⁵)

(Zheng et al., 2007)

abnormal stress response (with foxo²⁵)

(Tettweiler et al., 2005)

decreased body size (with foxo²⁵)

(Kramer et al., 2008)

decreased cell number | adult stage (with foxo²⁵)

(Koh et al., 2012)

increased cell death, with Scer\GAL4^ey.PU

(Hwang et al., 2013)

increased speed of aging (with foxo²⁵)

(Demontis and Perrimon, 2010)

short lived (with foxo²⁵)

(Sun et al., 2012)

short lived | nutrition conditional (with foxo²⁵)

(Kramer et al., 2008)

viable

(Junger et al., 2003)

visible, with Scer\GAL4^ey.PU

(Hwang et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

foxo²¹/foxo²⁵ mutant testes contain significantly more undifferentiated germ cells compared to heterozygous controls.

foxo²¹/foxo²⁵ mutant germaria show increased germline stem cell loss following irradiation compared with controls.

(Xing et al., 2015)

Adult brains have a significant decrease in dopaminergic neuron number (especially in the DL1 cluster) in 30 day old foxo²¹/foxo²⁵ flies. Climbing ability in foxo²¹/foxo²⁵ is significantly worse (at both 3 and even more so at 15 days old) and there is a significant reduction in ATP of the indirect flight muscle compared to wild type at 15 (but not 3) days old.

(Koh et al., 2012)

foxo²¹ mutant embryos do not exhibit aberrant cell fate specification or axon guidance defects. foxo²¹ mutant third instar larvae are indistinguishable from wild-type with respect to overall body size and muscle area and display no appreciable change in bouton number. However, the area of individual type 1b boutons is significantly increased. In addition, there is an increase in the number of closed, tightly bundled futsch-positive microtubule loops at neuromuscular junction 6/7.

foxo²¹ mutant neuromuscular junctions display enhanced microtubule stability compared to controls.

foxo²¹ mutants display impaired synaptic vesicle dynamics (assayed through FM 1-43 dye).

(Nechipurenko and Broihier, 2012)

foxo²¹/foxo²⁵ flies are short lived relative to wild-type controls, but their lifespan patterns responding to diet are similar to their wild-type controls.

(Sun et al., 2012)

The shift to a more acidic pH of fecal excreta which is seen in wild-type flies in response to a low-calorie diet does not occur in foxo²¹/Df(3R)Exel8159 flies.

(Cognigni et al., 2011)

foxo²¹/foxo²⁵ mutant animals display accelerated muscle aging manifesting in the increased accumulation of age-related protein aggregates compared with wild-type flies.

(Demontis and Perrimon, 2010)

foxo²¹/foxo²⁵ transheterozygous larvae exhibit reduced survival and shorter life span under amino acid starvation conditions. These flies are also developmentally delayed under these conditions and are smaller than wild-type after 48 hours.

(Kramer et al., 2008)

Starvation-induced autophagy is strongly reduced compared to controls in foxo²¹/foxo²⁵ larvae.

(Juhász et al., 2007)

foxo²¹/foxo²⁵ mutants rapidly become arrythmic in the presence of low levels of paraquat (0.5-1mM), while wild-type flies retain their rhythms for a few weeks. Phototaxis is not affected by paraquat treatment in these mutants.

foxo²¹/foxo²⁵ mutant flies are hypersensitive to oxidative stress.

30 day old foxo²¹/foxo²⁵ mutant flies show weak rhythms simliar to those seen in response to paraquat in young flies.

(Zheng et al., 2007)

foxo^BG01018/foxo²¹ flies display a 4.8% improvement in medial survival following M.marinum infection in comparison to wild-type flies - 174 hours vs. 166 hours. foxo²¹/foxo²⁵ transheterozygotes survive even longer - 190 hours following infection. The number of bacteria recorded in these flies is not significantly different from wild-type flies.

(Dionne et al., 2006)

Homozygous mutant animals do not exhibit a a detectable growth phenotype.

(Colombani et al., 2005)

foxo²¹/foxo²⁵ flies exhibit a reduced life-span when exposed to 5% hydrogen peroxide-containing media (median life-span of 34.5 hours). Only 2% of foxo²¹/foxo²⁵ flies survived 60 hours after exposure to oxidative stress, compared to 66.1% in wild-type.

(Tettweiler et al., 2005)

Shows no obvious phenotype under normal culturing conditions, though close inspection reveals the wing size is slightly reduced. Clonal analysis in the head capsule reveals no effect on growth. Clonal analysis reveals no difference of cell size in the developing eye between mutant and wild type. No significant difference between body weight of mutant and wild type flies is detectable. When placed on hydrogen-peroxide-containing food mutant flies display significantly reduced survival time compared to control flies. A similar effect occurs in response to paraquat feeding.

(Junger et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

foxo²⁵/foxo²¹ has decreased cell number | adult stage phenotype, non-enhanceable by Pink1^B9

(Koh et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, foxo²¹ has visible phenotype, suppressible by dj-1β^UAS.Tag:HA/Scer\GAL4^ey.PU

(Hwang et al., 2013)

Scer\GAL4^ey.PU, foxo²¹ has increased cell death phenotype, suppressible by dj-1β^UAS.Tag:HA/Scer\GAL4^ey.PU

(Hwang et al., 2013)

foxo²⁵/foxo²¹ has abnormal stress response phenotype, suppressible by Scer\GAL4^Act5C.PU/Thor^UAS.cTa

(Tettweiler et al., 2005)

Enhancer of

Statement

Reference

foxo²⁵/foxo²¹ is an enhancer of decreased cell size phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

NOT Enhancer of

Statement

Reference

foxo²⁵/foxo²¹ is a non-enhancer of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of increased cell death | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Suppressor of

Statement

Reference

foxo²¹ is a suppressor of increased cell death | third instar larval stage phenotype of Lsd-2^GD14108, Scer\GAL4^Bx-MS1096-KE

(Binh et al., 2019)

foxo²¹ is a suppressor | partially of visible | adult stage phenotype of Lsd-2^GD14108, Scer\GAL4^Bx-MS1096-KE

(Binh et al., 2019)

foxo²⁵/foxo²¹ is a suppressor of decreased cell number | oogenesis phenotype of InR^E19/InR³³⁹

(Su et al., 2018)

foxo²⁵/foxo²¹ is a suppressor of abnormal oxidative stress response | adult stage | chemical conditional phenotype of Trap1^EY21851

(Kim et al., 2016)

foxo²¹/foxo[+] is a suppressor | partially of increased cell death | third instar larval stage phenotype of Hsap\APLP1^UAS.cMa, Scer\GAL4^ptc-559.1

(Wang et al., 2015)

foxo²¹/foxo[+] is a suppressor of visible phenotype of Hsap\APLP1^UAS.cMa, Scer\GAL4^sd-SG29.1

(Wang et al., 2015)

foxo²¹/foxo[+] is a suppressor of visible phenotype of Hsap\APLP1^UAS.cMa, Scer\GAL4^pnr-MD237

(Wang et al., 2015)

foxo²¹/foxo[+] is a suppressor | partially of abnormal size | adult stage phenotype of Ide^UAS.cGa, Scer\GAL4^Bx-MS1096-KE

(Galagovsky et al., 2014)

foxo²¹/foxo[+] is a suppressor | partially of abnormal size | adult stage phenotype of Ide^UAS.cGa, Scer\GAL4^en-e16E

(Galagovsky et al., 2014)

foxo²¹/foxo[+] is a suppressor of abnormal size phenotype of Ide^GD6073, Scer\GAL4^en-e16E

(Galagovsky et al., 2014)

foxo²¹ is a suppressor of visible phenotype of Daxx^EY09290, Scer\GAL4^Bx-MS1096

(Hwang et al., 2013)

foxo²¹/foxo[+] is a suppressor of increased cell death | larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^elav.PU

(Hong et al., 2012)

foxo²¹/foxo[+] is a suppressor of lethal phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^elav.PU

(Hong et al., 2012)

foxo²¹/foxo[+] is a suppressor of abnormal locomotor behavior phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^elav.PU

(Hong et al., 2012)

foxo²¹/foxo[+] is a suppressor | partially of short lived | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, for^P1.UAS

(Kanao et al., 2012)

foxo²¹/foxo[+] is a suppressor | partially of abnormal locomotor behavior | adult stage | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, for^P1.UAS

(Kanao et al., 2012)

Scer\GAL4^ple.PF, Sirt1^EP2300, foxo²¹, foxo[+] is a suppressor | partially of decreased cell number | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²¹/foxo[+] is a suppressor | partially of visible phenotype of Cln3^UAS.cTa, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

foxo²¹ is a suppressor of abnormal body size | recessive phenotype of 14-3-3ε^j2B10

(Nielsen et al., 2008)

foxo²¹/foxo[+] is a suppressor of long lived | dominant phenotype of 14-3-3ε^j2B10

(Nielsen et al., 2008)

foxo²¹ is a suppressor of decreased cell number phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206

(Colombani et al., 2005)

foxo²¹ is a suppressor of decreased cell size phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206

(Colombani et al., 2005)

foxo²¹ is a suppressor of decreased body size phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206

(Colombani et al., 2005)

foxo²¹ is a suppressor of abnormal cell growth phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206

(Colombani et al., 2005)

foxo²¹/foxo[+] is a suppressor of long lived | dominant phenotype of puc^E69

(Wang et al., 2005)

foxo²¹/foxo[+] is a suppressor of decreased cell number phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of decreased cell number phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²¹/foxo[+] is a suppressor of visible phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of visible phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor | partially of decreased body size phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of lethal | larval stage phenotype of Akt¹

(Junger et al., 2003)

NOT Suppressor of

Statement

Reference

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of increased cell death | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²¹/foxo[+] is a non-suppressor of long lived phenotype of Scer\GAL4^Mhc.PW, Thor^LL.UAS

(Demontis and Perrimon, 2010)

foxo²¹/foxo[+] is a non-suppressor of decreased cell size phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

Other

Statement

Reference

Pink1^B9, Trap1^EY21851, foxo²¹/foxo[+] has abnormal locomotor behavior | adult stage phenotype

(Kim et al., 2016)

Crtc^25-3, foxo²¹, foxo²⁵ has lethal phenotype

(Wang et al., 2008)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

foxo²⁵/foxo²¹ has dorso-lateral dopaminergic neuron phenotype, non-enhanceable by Pink1^B9

(Koh et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, foxo²¹ has eye phenotype, suppressible by dj-1β^UAS.Tag:HA/Scer\GAL4^ey.PU

(Hwang et al., 2013)

foxo²¹ has type I bouton | increased number phenotype, suppressible by futsch^K68/futsch[+]

(Nechipurenko and Broihier, 2012)

foxo²¹ has embryonic/larval neuromuscular junction phenotype, suppressible by futsch^K68/futsch[+]

(Nechipurenko and Broihier, 2012)

foxo²¹ has synaptic vesicle phenotype, suppressible by futsch^K68/futsch[+]

(Nechipurenko and Broihier, 2012)

foxo²¹ has microtubule phenotype, suppressible by futsch^K68/futsch[+]

(Nechipurenko and Broihier, 2012)

foxo²¹ has microtubule phenotype, suppressible by futsch^N94/futsch[+]

(Nechipurenko and Broihier, 2012)

Enhancer of

Statement

Reference

foxo²⁵/foxo²¹ is an enhancer of ommatidium phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

NOT Enhancer of

Statement

Reference

foxo²⁵/foxo²¹ is a non-enhancer of adult thorax phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of indirect flight muscle cell phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of wing blade phenotype of Pink1^B9

(Koh et al., 2012)

Suppressor of

Statement

Reference

foxo²¹ is a suppressor of wing pouch | third instar larval stage phenotype of Lsd-2^GD14108, Scer\GAL4^Bx-MS1096-KE

(Binh et al., 2019)

foxo²¹ is a suppressor | partially of wing phenotype of Lsd-2^GD14108, Scer\GAL4^Bx-MS1096-KE

(Binh et al., 2019)

foxo²⁵/foxo²¹ is a suppressor of escort cell phenotype of InR^E19/InR³³⁹

(Su et al., 2018)

foxo²¹/foxo[+] is a suppressor | partially of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Hsap\APLP1^UAS.cMa, Scer\GAL4^ptc-559.1

(Wang et al., 2015)

foxo²¹/foxo[+] is a suppressor of wing blade | adult stage phenotype of Hsap\APLP1^UAS.cMa, Scer\GAL4^sd-SG29.1

(Wang et al., 2015)

foxo²¹/foxo[+] is a suppressor of scutellum | adult stage phenotype of Hsap\APLP1^UAS.cMa, Scer\GAL4^pnr-MD237

(Wang et al., 2015)

foxo²¹/foxo[+] is a suppressor | partially of wing phenotype of Ide^UAS.cGa, Scer\GAL4^Bx-MS1096-KE

(Galagovsky et al., 2014)

foxo²¹/foxo[+] is a suppressor | partially of wing phenotype of Ide^UAS.cGa, Scer\GAL4^en-e16E

(Galagovsky et al., 2014)

foxo²¹/foxo[+] is a suppressor of wing blade posterior compartment phenotype of Ide^GD6073, Scer\GAL4^en-e16E

(Galagovsky et al., 2014)

foxo²¹ is a suppressor of wing phenotype of Daxx^EY09290, Scer\GAL4^Bx-MS1096

(Hwang et al., 2013)

foxo²¹/foxo[+] is a suppressor of eye phenotype of Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Hong et al., 2012)

Scer\GAL4^ple.PF, Sirt1^EP2300, foxo²¹, foxo[+] is a suppressor | partially of dorso-lateral dopaminergic neuron phenotype of Pink1^B9

(Koh et al., 2012)

Scer\GAL4^ple.PF, Sirt1^EP2300, foxo²¹, foxo[+] is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²¹/foxo[+] is a suppressor | partially of eye phenotype of Cln3^UAS.cTa, Scer\GAL4^GMR.PF

(Tuxworth et al., 2011)

foxo²¹/foxo[+] is a suppressor of eye phenotype of Scer\GAL4^sev.EP, hep^Act.UAS

(Wang et al., 2005)

foxo²¹/foxo[+] is a suppressor of ommatidium phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of ommatidium phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²¹/foxo[+] is a suppressor of wing phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of wing phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

NOT Suppressor of

Statement

Reference

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of adult thorax phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of wing blade phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-suppressor of eye phenotype of Crtc^UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2008)

foxo²¹/foxo[+] is a non-suppressor of ommatidium phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of escort cells observed in the germarium of InR^E19/InR³³⁹ transheterozygotes is suppressed by additional foxo²¹/foxo²⁵ transheterozygosity.

(Su et al., 2018)

Presence of foxo²⁵/foxo²¹ significantly suppresses increased survival in response to rotenone or paraquat in Trap1^EY21851/Trap1^EY21851 flies. foxo²¹/+ partially suppresses rescue of locomotor defects by Trap1^EY21851/Trap1^EY21851 in Pink1^B9/Pink1^B9 flies. Presence of foxo²⁵/foxo²¹ suppresses rescue via feeding of G-TTP of locomotor defects in Pink1^B9/Pink1^B9 flies.

(Kim et al., 2016)

The reduced wing phenotype caused by expression of DLP^EY09290 under the control of Scer\GAL4^Bx-MS1096 is suppressed by foxo²¹.

(Hwang et al., 2013)

The defect in climbing ability and reduction in lifespan seen in flies expressing for^P1.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 is partially suppressed by foxo²¹/+.

(Kanao et al., 2012)

Expression of Sirt1^Scer\UAS.cGa driven by Scer\GAL4^arm.PU partially suppresses phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in (3 day old) Pink1^B9/Y flies. Presence of foxo²⁵/foxo²¹ almost completely nullifies the suppressive effect of Scer\GAL4^arm.PU>Sirt1^Scer\UAS.cGa on phenotypes in Pink1^B9/Y flies.

foxo²⁵/foxo²¹ does not significantly enhance phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in Pink1^B9/Y flies.

Pink1^B9/Y does not enhance dopaminergic neuron loss (in the DL1 cluster) in foxo²¹/foxo²⁵ flies.

(Koh et al., 2012)

A futsch^K68/+ background suppresses the increase in type 1b bouton area observed in foxo²¹ homozygotes. Furthermore, this background dominantly suppresses the elevated number of microtubule loops present in foxo²¹ mutants.

A futsch^N94 background dominantly suppresses the elevated number of microtubule loops present in foxo²¹ mutants.

A futsch^K68/+ background suppresses the FM 1-43 loading defects found in foxo²¹ mutant synapses.

(Nechipurenko and Broihier, 2012)

foxo²¹ does not rescue the reduced body weight of Lst8¹ flies.

(Wang et al., 2012)

The eye phenotype resulting from the overexpression of cln3^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF is partially suppressed by foxo²¹/+.

(Tuxworth et al., 2011)

Lipid levels are completely restored in Sik3⁴⁸ ; foxo²¹/foxo²⁵ double mutant flies.

(Wang et al., 2011)

Overexpression of Thor^LL.Scer\UAS under the control of Scer\GAL4^Mhc.PW in a heterozygous foxo²¹ mutant genetic background is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

(Demontis and Perrimon, 2010)

The 'food-leaving' behaviour of for^R, foxo²¹ flies is not significantly different from for^R flies.

(Kent et al., 2009)

The lifespan of 14-3-3ε^j2B10/+, foxo²¹/+ double heterozygous flies is similar to wild-type levels.

(Nielsen et al., 2008)

Removal of both copies of foxo (foxo²⁵/foxo²¹) is lethal in TORC^25-3 homozygotes.

TORC^Scer\UAS.cWa over-expressing flies (driven by Scer\GAL4^GMR.PF) in which foxo is reduced or eliminated though a foxo²⁵/foxo²¹ background still exhibit a rough eye phenotype.

(Wang et al., 2008)

Ectopic expression of Thor^Scer\UAS.cTa, under the control of Scer\GAL4^Act5C can completely suppress the sensitivity of foxo²¹/foxo²⁵ flies to oxidative stress (median life-span of 56.8 hours, 39.7% survival rate after 60 hours exposure to 5% hydrogen peroxide).

(Tettweiler et al., 2005)

The increase in average and maximum lifespan seen in puc^E69/+ adults is dominantly suppressed by foxo²¹.

(Wang et al., 2005)

Xenogenetic Interactions

Statement

Reference

foxo²¹/+ significantly partially suppresses the increased cell death induced by expression of Hsap\APLP1^Scer\UAS.cMa driven along the anterior/posterior compartment boundary in third instar larval wing discs by Scer\GAL4^ptc-559.1, suppresses the blistered wing phenotype in flies with expression of Hsap\APLP1^Scer\UAS.cMa driven by Scer\GAL4^sd-SG29.1, and suppresses the small scutellum phenotype seen with expression of Hsap\APLP1^Scer\UAS.cMa driven by Scer\GAL4^pnr-MD237.

(Wang et al., 2015)

The ability of 0.5mM Psammaplysene A to suppress the eye degeneration phenotype caused by expression of Hsap\AR^Q52.Scer\UAS under the control of Scer\GAL4^GMR.PU (in the presence of dihydrotestosterone) is suppressed if the flies are also heterozygous for foxo²¹.

(Mojsilovic-Petrovic et al., 2009)

Complementation and Rescue Data

Rescued by

foxo²⁵/foxo²¹ is rescued by foxo^UAS.cFa/Scer\GAL4^Lk6-DJ634

(Zheng et al., 2007)

foxo²⁵/foxo²¹ is rescued by foxo^Tag:V5

(Zheng et al., 2007)

Not rescued by

foxo²⁵/foxo²¹ is not rescued by foxo^UAS.cFa/Scer\GAL4^P2.4.Pdf

(Zheng et al., 2007)

foxo²⁵/foxo²¹ is not rescued by foxo^Tag:V5

(Zheng et al., 2007)

Comments

Expression of foxo^T:SV5\V5 rescues the circadian arrhythmia seen in foxo²¹/foxo²⁵ mutants. Phototaxis is not affected by paraquat treatment in these mutants.

Expression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Lk6-DJ634 rescues the circadian arrhythmia seen in foxo²¹/foxo²⁵ mutants. Phototaxis is not affected by paraquat treatment in these mutants.

(Zheng et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

80943

y¹ w^*; foxo²¹/TM6B, Tb¹

80949

y¹ w^*; P{UAS-foxo.F}3, foxo²¹/TM3, Sb¹

80952

y¹ w^*; foxo²¹ P{EPgy2}Akt^EY10012/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

FOXO²¹

(Kim et al., 2016, Galagovsky et al., 2014, Koh et al., 2012, Wang et al., 2011, Francis et al., 2010, Teleman et al., 2005)

Foxo²¹

(Juhász et al., 2007)

dFOXO²¹

(Mahoney et al., 2016, Liu et al., 2014, Hwang et al., 2013, Hong et al., 2012, Chung et al., 2010, Vihervaara and Puig, 2008, Colombani et al., 2005, Puig and Tjian, 2005, Junger et al., 2003)

dFoxO²¹

(Kanao et al., 2012, Kanao et al., 2010)

dFoxo²¹

(Kenmoku et al., 2017, Tokusumi et al., 2012)

dFoxo^Rw21

(Slaidina et al., 2009)

dfoxO²¹

(Binh et al., 2019, Wang et al., 2015)

dfoxo21

(Nielsen et al., 2008)

dfoxo21a

(Slack et al., 2010)

dfoxo²¹

(Sun et al., 2012, Buch et al., 2008, Nielsen et al., 2008)

foxo21

(Zid et al., 2009)

foxo^21a

(Mensah et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (54)

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