|Feature type||allele||Associated gene||Dmel\foxo|
|Also Known As||dFOXO21|
|Map ( GBrowse )|
|Allele class||loss of function allele|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: W95@.
|Caused by insertion|
|Phenotype Manifest In|
foxo mutant embryos do not exhibit aberrant cell fate specification or axon guidance defects. foxo mutant third instar larvae are indistinguishable from wild-type with respect to overall body size and muscle area and display no appreciable change in bouton number. However, the area of individual type 1b boutons is significantly increased. In addition, there is an increase in the number of closed, tightly bundled futsch-positive microtubule loops at neuromuscular junction 6/7. foxo mutant neuromuscular junctions display enhanced microtubule stability compared to controls. foxo mutants display impaired synaptic vesicle dynamics (assayed through FM 1-43 dye).
foxo/foxo flies are short lived relative to wild-type controls, but their lifespan patterns responding to diet are similar to their wild-type controls.
The shift to a more acidic pH of fecal excreta which is seen in wild-type flies in response to a low-calorie diet does not occur in foxo/Df(3R)Exel8159 flies.
foxo/foxo mutant animals display accelerated muscle aging manifesting in the increased accumulation of age-related protein aggregates compared with wild-type flies.
foxo/foxo transheterozygous larvae exhibit reduced survival and shorter life span under amino acid starvation conditions. These flies are also developmentally delayed under these conditions and are smaller than wild-type after 48 hours.
foxo/foxo mutants rapidly become arrythmic in the presence of low levels of paraquat (0.5-1mM), while wild-type flies retain their rhythms for a few weeks. Phototaxis is not affected by paraquat treatment in these mutants. foxo/foxo mutant flies are hypersensitive to oxidative stress. 30 day old foxo/foxo mutant flies show weak rhythms simliar to those seen in response to paraquat in young flies.
foxoBG01018/foxo21 flies display a 4.8% improvement in medial survival following M.marinum infection in comparison to wild-type flies - 174 hours vs. 166 hours. foxo21/foxo25 transheterozygotes survive even longer - 190 hours following infection. The number of bacteria recorded in these flies is not significantly different from wild-type flies.
Homozygous mutant animals do not exhibit a a detectable growth phenotype.
foxo21/foxo25 flies exhibit a reduced life-span when exposed to 5% hydrogen peroxide-containing media (median life-span of 34.5 hours). Only 2% of foxo21/foxo25 flies survived 60 hours after exposure to oxidative stress, compared to 66.1% in wild-type.
Shows no obvious phenotype under normal culturing conditions, though close inspection reveals the wing size is slightly reduced. Clonal analysis in the head capsule reveals no effect on growth. Clonal analysis reveals no difference of cell size in the developing eye between mutant and wild type. No significant difference between body weight of mutant and wild type flies is detectable. When placed on hydrogen-peroxide-containing food mutant flies display significantly reduced survival time compared to control flies. A similar effect occurs in response to paraquat feeding.
foxo21/foxo[+] is a suppressor | partially of locomotor behavior defective | adult stage | RU486 conditional phenotype of Scer\GAL4elav.Switch.PO, forP1.Scer\UAS
foxo21/foxo[+] is a suppressor | partially of short lived | RU486 conditional phenotype of Scer\GAL4elav.Switch.PO, forP1.Scer\UAS
foxo21/foxo[+] is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, cln3Scer\UAS.cTa
foxo21 is a suppressor of cell growth defective phenotype of Pi3K92EScer\UAS.T:Hsap\MYC, Scer\GAL4P0206
foxo21 is a suppressor of decreased cell number phenotype of Pi3K92EScer\UAS.T:Hsap\MYC, Scer\GAL4P0206
foxo21 is a suppressor of decreased cell size phenotype of Pi3K92EScer\UAS.T:Hsap\MYC, Scer\GAL4P0206
|NOT Suppressor of|
|Phenotype Manifest In|
foxo21/foxo[+] is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, cln3Scer\UAS.cTa
|NOT Suppressor of|
The defect in climbing ability and reduction in lifespan seen in flies expressing for[P1.Scer\UAS] under the control of Scer\GAL4[elav.Switch.PO] in the presence of RU486 is partially suppressed by foxo/+.
A futsch[K68]/+ background suppresses the increase in type 1b bouton area observed in foxo homozygotes. Furthermore, this background dominantly suppresses the elevated number of microtubule loops present in foxo mutants. A futsch[N94] background dominantly suppresses the elevated number of microtubule loops present in foxo mutants. A futsch[K68]/+ background suppresses the FM 1-43 loading defects found in foxo mutant synapses.
The eye phenotype resulting from the overexpression of cln3[Scer\UAS.cTa] under the control of Scer\GAL4[GMR.PF] is partially suppressed by foxo/+.
Overexpression of Thor[LL.Scer\UAS] under the control of Scer\GAL4[Mhc.PW] in a heterozygous foxo mutant genetic background is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.
The 'food-leaving' behaviour of for[R], foxo flies is not significantly different from for[R] flies.
The lifespan of 14-3-3ε[j2B10]/+, foxo/+ double heterozygous flies is similar to wild-type levels.
Removal of both copies of foxo (foxo/foxo) is lethal in TORC[25-3] homozygotes. TORC[Scer\UAS.cWa] over-expressing flies (driven by Scer\GAL4[GMR.PF]) in which foxo is reduced or eliminated though a foxo/foxo background still exhibit a rough eye phenotype.
Ectopic expression of ThorScer\UAS.cTa, under the control of Scer\GAL4Act5C can completely suppress the sensitivity of foxo21/foxo25 flies to oxidative stress (median life-span of 56.8 hours, 39.7% survival rate after 60 hours exposure to 5% hydrogen peroxide).
|Complementation & Rescue Data|
|Not rescued by|
Expression of foxo[T:SV5\V5] rescues the circadian arrhythmia seen in foxo/foxo mutants. Phototaxis is not affected by paraquat treatment in these mutants. Expression of foxo[Scer\UAS.cFa] under the control of Scer\GAL4[Lk6-DJ634] rescues the circadian arrhythmia seen in foxo/foxo mutants. Phototaxis is not affected by paraquat treatment in these mutants.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 10 )|
|Secondary FlyBase IDs|
|References ( 33 )|
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|Recent research papers ( 9 )|