FlyBase Allele Report: Dmel\edl[UAS.cYa]

General Information

Symbol

Dmel\edl^UAS.cYa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Yamada

FlyBase ID

FBal0151997

Feature type

allele

Associated gene

Dmel\edl

Associated Insertion(s)

Carried in Construct

P{UAS-edl.Y}

Also Known As

UAS-edl

Key Links

Transgenic product class

cDNA

(Yamada et al., 2003)

wild_type

(Yamada et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Yamada et al., 2003)

wild_type

(Yamada et al., 2003)

Mutagen

in vitro construct

(Yamada et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-edl.Y}

Cytology

Description

UASt regulatory sequences drive expression of the edl open reading frame (cDNA sequence).

(Yamada et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

edl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | embryonic stage, with Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

visible, with Scer\GAL4^elav-C155

(Yamada et al., 2003)

visible, with Scer\GAL4^en-e16E

(Yamada et al., 2003)

visible, with Scer\GAL4^hs.2sev

(Yamada et al., 2003)

Phenotype Manifest In

dorsal appendage, with Scer\GAL4^CY2

(Yamada et al., 2003)

embryonic cardioblast | increased number, with Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

embryonic heart ostial cardioblast | increased number, with Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

eye, with Scer\GAL4^elav-C155

(Yamada et al., 2003)

eye photoreceptor cell, with Scer\GAL4^elav-C155

(Yamada et al., 2003)

heart primordium, with Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

ommatidial precursor cluster, with Scer\GAL4^elav-C155

(Yamada et al., 2003)

ommatidium, with Scer\GAL4^hs.2sev

(Yamada et al., 2003)

photoreceptor cell R7, with Scer\GAL4^hs.2sev

(Yamada et al., 2003)

wing, with Scer\GAL4^en-e16E

(Yamada et al., 2003)

Detailed Description

Statement

Reference

Embryos expressing edl^UAS.cYa under the control of Scer\GAL4^twi.2PE leads to cardioblast patterning defects and to a significant increase in the overall number of cardioblasts (both generic and ostial cardioblasts), as compared to controls.

(Schwarz et al., 2018)

Expression of edl^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 results in a reduction in eye size. Photoreceptors neurons are hardly detected and most of the retina is occupied by pigment cells. In the developing eye disc, a massive reduction of differentiating neurons is seen from the earliest stages of ommatidial assembly, although R8 specification is largely unaffected. Expression of edl^Scer\UAS.cYa under the control of Scer\GAL4^hs.2sev does not transform cone cells towards a neuronal fate and suppresses the formation of the endogenous R7 neurons. Expression of edl^Scer\UAS.cYa under the control of Scer\GAL4^hs.2sev results in a rough eye phenotype. Expression of edl^Scer\UAS.cYa under the control of Scer\GAL4^CY2 dorsalises the chorion, resulting in fused dorsal appendages. Expression of edl^Scer\UAS.cYa under the control of Scer\GAL4^en-e16E results in a duplication of the wing.

(Yamada et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, edl^UAS.cYa has visible phenotype, enhanceable by pnt[+]/pnt^Δ88

(Yamada et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, edl^UAS.cYa has ommatidium phenotype, enhanceable by pnt[+]/pnt^Δ88

(Yamada et al., 2003)

Suppressor of

Statement

Reference

edl^UAS.cYa, Scer\GAL4^hs.2sev is a suppressor of photoreceptor cell R7 | ectopic phenotype of Ras85D^V12.sev, Scer\GAL4^hs.2sev

(Yamada et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of edl^Scer\UAS.cYa under the control of Scer\GAL4^hs.2sev suppresses the ectopic R7 neurons caused by expression of Ras85D^V12.sev. The rough eye phenotype caused by expression of edl^Scer\UAS.cYa under the control of Scer\GAL4^hs.2sev is enhanced by pnt^Δ88/+.

(Yamada et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

edl^UAS.cYa/Scer\GAL4^tin.CΔ4 partially rescues edl^k06602

(Schwarz et al., 2018)

Scer\GAL4^tin.cBa/edl^UAS.cYa partially rescues edl^k06602

(Schwarz et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

603843

y¹ w^*; P{UAS-edl.Y}X

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

edl^Scer\UAS.cYa

edl^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Yamada

(Yamada et al., 2003)

Secondary FlyBase IDs

References (2)

Report Sections

Open Close