Allele Dmel\Vang^Scer\UAS.cWa

General Information
Symbol	Dmel\Vang^Scer\UAS.cWa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Wolff	FlyBase ID	FBal0152143
Feature type	allele	Associated gene	Dmel\Vang

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Jenny et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of Vang. (Jenny et al., 2003)
Carried in construct	P{UAS-stbm.W} (Simons et al., 2009, Courbard et al., 2009, Jenny et al., 2003, Klein et al., 2006, Lawrence et al., 2004, Jenny et al., 2005, Mirkovic et al., 2011, Hermle et al., 2013)
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective, with Scer\GAL4^hs.2sev (Jenny et al., 2003, Klein et al., 2006) planar polarity defective \| cell non-autonomous \| somatic clone, with Scer\GAL4^αTub84B.PL (Lawrence et al., 2004) planar polarity defective \| somatic clone, with Scer\GAL4^αTub84B.PL (Lawrence et al., 2004) viable (Lawrence et al., 2004) visible, with Scer\GAL4^dpp.blk1 (Jenny et al., 2005)
Phenotype Manifest In
	ommatidium, with Scer\GAL4^hs.2sev (Jenny et al., 2003, Klein et al., 2006) trichome & adult abdomen, with Scer\GAL4^αTub84B.PL (Lawrence et al., 2004) trichome & adult abdomen \| somatic clone, with Scer\GAL4^αTub84B.PL (Lawrence et al., 2004) trichome & adult abdomen \| somatic clone \| cell non-autonomous, with Scer\GAL4^αTub84B.PL (Lawrence et al., 2004) wing hair, with Scer\GAL4^dpp.blk1 (Jenny et al., 2005)
Detailed Description
	Statement Reference Expression of Vang[Scer\UAS.cWa] under the control of Scer\GAL4[dpp.blk1] only very rarely leads to cell orientation defects in the wing. (Courbard et al., 2009) Expression of Vang^Scer\UAS.cWa in the eye, under the control of Scer\GAL4^hs.2sev, causes mild planar polarity defects. (Klein et al., 2006) Expression of Vang^Scer\UAS.cWa under the control of Scer\GAL4^dpp.blk1 in a stripe along the proximo-distal axis of the wing results in a reorientation of wing hairs, which now point towards from the expression domain. (Jenny et al., 2005) Vang^Scer\UAS.cWa; Scer\GAL4^αTub84B.PL flies are viable and have largely normal planar polarity. In the abdomens of Vang^Scer\UAS.cWa; Scer\GAL80^αTub84B.PL; Scer\GAL4^αTub84B.PL flies, somatic clones lacking Scer\GAL80^αTub84B.PL affect planar polarity in two ways: (i) hairs behind the clone are usually reversed, but (ii) hairs inside and mainly at the back of the clone are disheveled. The non-autonomous affect of these clones on tissue polarity can act across the anterior-posterior compartment boundary. (Lawrence et al., 2004) When Vang^Scer\UAS.cWa is driven by Scer\GAL4^hs.2sev at 29^oC, about 2% of ommatidia exhibit planar polarity defects. (Jenny et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, enhanceable by dco^j3B9/dco[+] (Klein et al., 2006) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, enhanceable by dgo³⁰⁸ (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, enhanceable by dgo³⁸⁰ (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, enhanceable by pk^pk-sple-13 (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, enhanceable by pk^pk-sple-26 (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, enhanceable by stan^E45 (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, enhanceable by stan^E59 (Jenny et al., 2003)
NOT Enhanced by
Statement Reference Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, non-enhanceable by N^55e11 (Jenny et al., 2003)
Suppressed by
Statement Reference Scer\GAL4^αTub84B.PL, Vang^Scer\UAS.cWa has planar polarity defective \| somatic clone \| cell non-autonomous phenotype, suppressible by stan^E59/stan^E59 (Lawrence et al., 2004)
NOT suppressed by
Statement Reference Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has cell polarity defective phenotype, non-suppressible by N^55e11 (Jenny et al., 2003)
Enhancer of
Statement Reference Scer\GAL4^hs.2sev/Vang^Scer\UAS.cWa is an enhancer of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011)
Other
Statement Reference Scer\GAL4^dpp.blk1, Vang^Scer\UAS.cWa, scrib^{Scer\UAS.P\T.T:Avic\GFP} has planar polarity defective phenotype (Courbard et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, enhanceable by dco^j3B9/dco[+] (Klein et al., 2006) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, enhanceable by dgo³⁰⁸ (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, enhanceable by dgo³⁸⁰ (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, enhanceable by pk^pk-sple-13 (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, enhanceable by pk^pk-sple-26 (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, enhanceable by stan^E45 (Jenny et al., 2003) Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, enhanceable by stan^E59 (Jenny et al., 2003)
NOT Enhanced by
Statement Reference Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, non-enhanceable by N^55e11 (Jenny et al., 2003)
Suppressed by
Statement Reference Scer\GAL4^αTub84B.PL, Vang^Scer\UAS.cWa has trichome & adult abdomen \| somatic clone \| cell non-autonomous phenotype, suppressible by stan^E59/stan^E59 (Lawrence et al., 2004)
NOT suppressed by
Statement Reference Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa has ommatidium phenotype, non-suppressible by N^55e11 (Jenny et al., 2003)
Enhancer of
Statement Reference Scer\GAL4^hs.2sev/Vang^Scer\UAS.cWa is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011)
Other
Statement Reference Scer\GAL4^dpp.blk1, Vang^Scer\UAS.cWa, scrib^{Scer\UAS.P\T.T:Avic\GFP} has wing hair phenotype (Courbard et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Vang[Scer\UAS.cWa] enhances the ommatidial over-rotation phenotype which is caused by expression of nmo[Scer\UAS.cUa] under the control of Scer\GAL4[hs.2sev]. (Mirkovic et al., 2011) Co-expression of scrib[Scer\UAS.P\T.T:Avic\GFP] and Vang[Scer\UAS.cWa] under the control of Scer\GAL4[dpp.blk1] in the wing results in robust reorientation of cells outside the expression domain towards the expression domain. (Courbard et al., 2009) The planar polarity defects seen in the eye when Vang^Scer\UAS.cWa is expressed under the control of Scer\GAL4^hs.2sev in a wild-type background are significantly enhanced in a dco^j3B9/+ background. (Klein et al., 2006) The non-autonomous effect on planar polarity of Vang^Scer\UAS.cWa; Scer\GAL4^αTub84B.PL somatic clones (produced by the Scer\GAL80 MARCM method) in the adult abdomen is suppressed by pwn/pwn stan^E59/stan^E59. Cells within the resulting clones have randomised planar polarity. (Lawrence et al., 2004) The addition of pk^pk-sple-13 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 20% of ommatidia exhibit show defects. The addition of pk^pk-sple-26 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 14% of ommatidia exhibit show defects. The addition of dgo³⁰⁸ to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals partially enhances the planar polarity defects - about 5% of ommatidia exhibit show defects. The addition of dgo³⁸⁰ to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 12% of ommatidia exhibit show defects. The addition of stan^E45 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 25% of ommatidia exhibit show defects. The addition of stan^E59 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 25% of ommatidia exhibit show defects. The addition of N^55e11 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals has no effect on the planar polarity defects. (Jenny et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Vang^Scer\UAS.cWa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Wolff (Jenny et al., 2003)
Secondary FlyBase IDs

References ( 8 )
Research paper	Hermle et al., 2013, EMBO J. 32(2): 245--259 Drosophila ATP6AP2/VhaPRR functions both as a novel planar cell polarity core protein and a regulator of endosomal trafficking. [FBrf0220613] Courbard et al., 2009, Dev. Biol. 333(1): 67--77 The apical/basal-polarity determinant Scribble cooperates with the PCP core factor Stbm/Vang and functions as one of its effectors. [FBrf0208559] Simons et al., 2009, Nat. Cell Biol. 11(3): 286--294 Electrochemical cues regulate assembly of the Frizzled/Dishevelled complex at the plasma membrane during planar epithelial polarization. [FBrf0207478] Klein et al., 2006, Curr. Biol. 16(13): 1337--1343 CKIepsilon/discs overgrown promotes both Wnt-Fz/beta-catenin and Fz/PCP signaling in Drosophila. [FBrf0191849] Jenny et al., 2005, Nat. Cell Biol. 7(7): 691--697 Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. [FBrf0188147] Lawrence et al., 2004, Development 131(19): 4651--4664 Cell interactions and planar polarity in the abdominal epidermis of Drosophila. [FBrf0180148] Jenny et al., 2003, EMBO J. 22(17): 4409--4420 Prickle and Strabismus form a functional complex to generate a correct axis during planar cell polarity signaling. [FBrf0162094]
Supplementary material	Mirkovic et al., 2011, Nat. Struct. Mol. Biol. 18(6): Supplemental Data. [FBrf0213975]

Allele Dmel\VangScer\UAS.cWa

Allele Dmel\Vang^Scer\UAS.cWa