Allele Dmel\Sra-1^85.1

General Information
Symbol	Dmel\Sra-1^85.1	Species	D. melanogaster
Name		FlyBase ID	FBal0152179
Feature type	allele	Associated gene	Dmel\Sra-1
Also Known As	CYFIP^Δ85.1, CYFIP^85.1

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Schenck et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Sra-1^EP3267 (Schenck et al., 2003)
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Galy et al., 2011) lethal \| embryonic stage \| germline clone (Schenck et al., 2003) lethal \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) lethal \| pupal stage \| recessive (Schenck et al., 2003) neuroanatomy defective \| dominant (Schenck et al., 2004) neuroanatomy defective \| dominant \| larval stage (Qurashi et al., 2007) visible \| adult stage, with Scer\GAL4^lz-gal4, Sra-1^{dsRNA.Scer\UAS.cGa} (Galy et al., 2011) visible \| adult stage \| somatic clone (Galy et al., 2011) visible \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011)
Phenotype Manifest In
	axon (Schenck et al., 2004) bouton (Schenck et al., 2003) commissure (Schenck et al., 2003) cone cell \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) cone cell \| pupal stage (with Sra-1^EP3267) (Galy et al., 2011) embryonic/larval neuromuscular junction (Qurashi et al., 2007) eye \| adult stage, with Scer\GAL4^lz-gal4, Sra-1^{dsRNA.Scer\UAS.cGa} (Galy et al., 2011) eye \| adult stage \| somatic clone (Galy et al., 2011) eye \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) interommatidial bristle \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) interommatidial bristle \| pharate adult stage \| somatic clone (Galy et al., 2011) intersegmental nerve (Schenck et al., 2004, Schenck et al., 2003) lens \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) lens \| pharate adult stage \| somatic clone (Galy et al., 2011) longitudinal connective (Schenck et al., 2003) neuromuscular junction & synapse (Schenck et al., 2004) ommatidium \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) ommatidium \| pharate adult stage \| somatic clone (Galy et al., 2011) photoreceptor \| adult stage, with Scer\GAL4^lz-gal4, Sra-1^{dsRNA.Scer\UAS.cGa} (Galy et al., 2011) photoreceptor \| adult stage \| somatic clone (Galy et al., 2011) photoreceptor \| pupal stage (with Sra-1^EP3267) (Galy et al., 2011) presumptive embryonic/larval central nervous system \| germline clone (Schenck et al., 2003) retina \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) retina \| pharate adult stage \| somatic clone (Galy et al., 2011) rhabdomere \| adult stage \| somatic clone (Galy et al., 2011) rhabdomere \| pharate adult stage (with Sra-1^EP3267) (Galy et al., 2011) rhabdomere \| pupal stage (with Sra-1^EP3267) (Galy et al., 2011) synapse (Schenck et al., 2003)
Detailed Description
	Statement Reference Sra-1[EP3267]/Sra-1[85.1] mutant pharate adults have rough eyes and display dark lenses in a speckled, random pattern indicating cone cell death. The ommatidia are occasionally misaligned or fused to their neighbours and some lack the typical smooth surface, instead showing crater-like intrusions indicative of lens material loss. Interommatidial bristles are often bent and appear soft and flat. Rhabdomeres appear bulky and never span the entire retina, either accumulating near the distal surface or underneath the retinal base (FM) in the lamina. The FM is mispositioned, leading to a markedly reduced retina depth. The rhabdomeres appear bulky and never span the entire retina. They either accumulate near the distal surface or underneath the retinal base (FM) in the lamina. The actin organisation of the FM is compromised and the polarised accumulation of dense cortical actin latices near the inner and outer lateral membrane of pigment cells is abolished. Over 30% of Sra-1[EP3267]/Sra-1[85.1] pupal photoreceptors possess ectopic membrane protrusions projecting from the PR stalks, a phenomenon never observed in wild type, and gaps occur between the rhabdomeres and the cytoplasm. The rhabdomeres comprise a massive amount of membrane that occupies an area that is twice as large as in controls. However the space occupied by the actin rich rhabdomere terminal web (RTW) is not proportionally increased. Actin fingers that normally extend from the sub-rhabdomeric space into the RTW are absent in Sra-1[EP3267]/Sra-1[85.1] mutant cells. Microvillar structure and stacking appear normal. Photoreceptor adherens junctions are correctly specified but structurally compromised. Defects in cone cell shape and configuration are also seen. Homozygous Sra-1[85.1] mutant eye clones lack pigmentation. The ommatidia are often misaligned or fused to their neighbours (on average 31 fusion events per eye) and some lack the typical smooth surface, instead showing crater-like intrusions indicative of lens material loss. Interommatidial bristles are often bent and appear soft and flat. Rhabdomeres appear bulky and never span the entire retina. They either accumulate near the distal surface or underneath the retinal base (FM) in the lamina. The FM is mispositioned, leading to a markedly reduced retina depth. (Galy et al., 2011) Heterozygous larvae show a significant reduction in synaptic length at the neuromuscular junction compared to wild type. (Qurashi et al., 2007) Mutant embryos show axon defects, including ectopic crossing of the midline by axons and ectopic branching of the intersegmental nerve. Sra-1^85.1 larvae have significantly shorter synapses at the neuromuscular junction compared to wild type. Heterozygous larvae have significantly shorter synapses at the neuromuscular junction (93.4 +/- 2.3 μm) compared to wild type (111.1 μm). (Schenck et al., 2004) Mutant embryos exhibit abnormal longitudinal connectives. They are seen to cross the midline, sometimes several times, they are not well separated and show occasional breaks. Commissures are also thicker than in wild-type, and are not properly separated. Intersegmental nerves are also seen to stall and exhibit abnormal branching. Mutant synapses in third instar larvae also exhibit abnormalities. Mutant synapses display undergrowth (synaptic length is reduced to 67-70% of wild-type length), disturbed bouton structure, and supernumerary budding, mutants exhibit four or five times more buds than controls. When embryos are produced that lack both the maternal and zygotic Sra-1, the embryonic central nervous system is completely disrupted, embryonic lethality is also observed. (Schenck et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Sra-1[+]/Sra-1^85.1 is a suppressor of neuroanatomy defective \| dominant phenotype of Fmr1^unspecified (Schenck et al., 2004)
Phenotype Manifest In
Suppressor of
Statement Reference Sra-1[+]/Sra-1^85.1 is a suppressor of neuromuscular junction & synapse phenotype of Fmr1^unspecified (Schenck et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference The overgrown synapse phenotype seen at the neuromuscular junction of Fmr1^unspecified/+ larvae is suppressed by one copy of Sra-1^85.1. (Schenck et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Sra-1^85.1 is rescued by Scer\GAL4^elav-C155/Sra-1^Scer\UAS.cSa (Schenck et al., 2003, Schenck et al., 2004)
Comments	Expression of Sra-1[EP3267] under the control of Scer\GAL4[GMR.PF] fully rescues the eye morphology defects seen in Sra-1[EP3267]/Sra-1[85.1] mutant pharate adults. The lethality associated with Sra-1[EP3267]/Sra-1[85.1] is not rescued. Expression of Sra-1[EP3267] under the control of Scer\GAL4[lz-gal4] fully rescues the retinal fenestrated membrane integrity defects seen in Sra-1[EP3267]/Sra-1[85.1] mutant pharate adults. As result photoreceptors are kept in place and no longer fall into the lamina. The dark necrotic lenses seen in mutant flies are also absent. Some bulky rhabdomeres are still seen, but fewer than are seen in the mutant alone. (Galy et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CYFIP^85.1 (Schenck et al., 2003, Galy et al., 2011) CYFIP^Δ85.1 (Schenck et al., 2004, Galy et al., 2011, Qurashi et al., 2007) Sra-1^85.1
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Galy et al., 2011, Dev. Biol. 359(1): 37--46 CYFIP dependent Actin Remodeling controls specific aspects of Drosophila eye morphogenesis. [FBrf0216378] Qurashi et al., 2007, Neural Dev. 2: 18 HSPC300 and its role in neuronal connectivity. [FBrf0205239] Schenck et al., 2004, Dev. Biol. 274(2): 260--270 WAVE/SCAR, a multifunctional complex coordinating different aspects of neuronal connectivity. [FBrf0179776] Schenck et al., 2003, Neuron 38(6): 887--898 CYFIP/Sra-1 controls neuronal connectivity in Drosophila and links the Rac1 GTPase pathway to the fragile X protein. [FBrf0160918]

Allele Dmel\Sra-185.1

Allele Dmel\Sra-1^85.1