At 25oC, the survival rates of homozygotes are: adult survival - 0%, eclosion - 0%, pupation 23%. At 18oC, the survival rates of homozygotes are: adult survival - 82%, eclosion - 85%, pupation 107%. Temperature shift experiments indicate that to survive after eclosion, Ptp69D18/Df(3L)8ex34 animals must be at the mid pupal stage prior to the shift to the restrictive temperature (25oC). The rate of eclosion drops from more than 80% at 18oC to less than 20% when Ptp69D18/Df(3L)8ex34 animals are shifted to the nonpermissive temperature prior to the onset of pupation. Temperature shift experiments also reveal a requirement for Ptp69D function during late embryonic development. Ptp69D18 embryos show motor axon defects; 15% of ISNb axons, 9% of ISN axons, 17% of SNa axons and 8% of SNc axons show defects.