Allele Dmel\slik^Scer\UAS.cHa

General Information
Symbol	Dmel\slik^Scer\UAS.cHa	Species	D. melanogaster
Name		FlyBase ID	FBal0152297
Feature type	allele	Associated gene	Dmel\slik

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Hipfner and Cohen, 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of slik. (Hipfner and Cohen, 2003)
Carried in construct	P{UAS-slik.H} (Hughes and Fehon, 2006, Hipfner and Cohen, 2003)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell number, with Scer\GAL4^ptc-559.1 (Hipfner and Cohen, 2003) increased cell number \| cell non-autonomous, with Scer\GAL4^ptc-559.1 (Hipfner and Cohen, 2003) mitotic cell cycle defective \| cell non-autonomous, with Scer\GAL4^ptc-559.1 (Hipfner and Cohen, 2003) small body, with Scer\GAL4^arm.PS, slik¹ (Hipfner and Cohen, 2003)
Phenotype Manifest In
	1st posterior cell, with Scer\GAL4^arm.PS (Hipfner and Cohen, 2003) peripodial epithelium, with Scer\GAL4^ptc-559.1 (Hipfner and Cohen, 2003)
Detailed Description
	Statement Reference When slik^Scer\UAS.cHa is driven by Scer\GAL4^ptc-559.1, overgrowth is seen in the region between wing veins 3 and 4, increasing the distance between these veins. When animals are grown at 18^oC, the area of this region increases by 13%, when grown at 25^oC the increase is only 5%. There is a considerable increase in BrdU incorporation compared to wild-type in the centre of the wing disc and in the peripodial cell layer overlying the wing disc in larvae expressing slik^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1. (Hipfner and Cohen, 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference slik^Scer\UAS.cHa has increased cell number phenotype, enhanceable by Scer\GAL4^ptc-559.1/BacA\p35^Scer\UAS.cHa (Hipfner and Cohen, 2003)
Suppressed by
Statement Reference Scer\GAL4^ptc-559.1, slik^Scer\UAS.cHa has increased cell number phenotype, suppressible \| partially by Dsor1^LH110 (Hipfner and Cohen, 2003) Scer\GAL4^ptc-559.1, slik^Scer\UAS.cHa has increased cell number phenotype, suppressible \| partially by phl¹² (Hipfner and Cohen, 2003) Scer\GAL4^ptc-559.1, slik^Scer\UAS.cHa has increased cell number phenotype, suppressible by phl⁷ (Hipfner and Cohen, 2003)
Other
Statement Reference BacA\p35^Scer\UAS.cHa, Scer\GAL4^ptc-559.1, slik^Scer\UAS.cHa has lethal phenotype (Hipfner and Cohen, 2003)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference When phl⁷ is added to slik^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 animals the overgrowth phenotype is completely suppressed. When phl¹² is added to slik^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 animals the overgrowth phenotype is partially suppressed. When Dsor1^LH110 is added to slik^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 animals the overgrowth phenotype is partially suppressed. (Hipfner and Cohen, 2003)
Xenogenetic Interactions
Statement Reference Genetic interaction (class, effect): increased cell number, enhanceable { BacA\p35^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 } When slik^Scer\UAS.cHa and BacA\p35^Scer\UAS.cHa are co-expressed under the control of Scer\GAL4^ptc-559.1 an enhancement of the slik^Scer\UAS.cHa, Scer\GAL4^ptc-559.1 overgrowth phenotype is seen. An increase in the number of cells with normal nuclear appearances are found in abnormal outgrowths below the epithelial layer of the wing disc. Adult escapers show overgrowth in the 1st posterior cell of the wing. (Hipfner and Cohen, 2003) When slik[Scer\UAS.cHa] and BacA\p35[Scer\UAS.cHa] are co-expressed under the control of Scer\GAL4[ptc-559.1] an enhancement of the slik[Scer\UAS.cHa], Scer\GAL4[ptc-559.1] overgrowth phenotype is seen. An increase in the number of cells with normal nuclear appearances are found in abnormal outgrowths below the epithelial layer of the wing disc. Adult escapers show overgrowth in the 1st posterior cell of the wing. (Hipfner and Cohen, 2003)
Complementation & Rescue Data
Partially rescues	Scer\GAL4^arm.PS/slik^Scer\UAS.cHa partially rescues slik¹ (Hipfner and Cohen, 2003)
Comments	slik¹ animals rescued with slik^Scer\UAS.cHa driven by Scer\GAL4^arm.PS develop to adulthood of reduced body size, with mildly rough eyes, but of otherwise normal appearance. (Hipfner and Cohen, 2003)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Plkk1^Scer\UAS.cHa slik^Scer\UAS.cHa
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Hughes and Fehon, 2006, J. Cell Biol. 175(2): 305--313 Phosphorylation and activity of the tumor suppressor Merlin and the ERM protein Moesin are coordinately regulated by the Slik kinase. [FBrf0193095] Hipfner et al., 2004, Genes Dev. 18(18): 2243--2248 Slik Sterile-20 kinase regulates Moesin activity to promote epithelial integrity during tissue growth. [FBrf0179743] Hipfner and Cohen, 2003, PLoS Biol. 1(2): e35 The Drosophila Sterile-20 Kinase Slik Controls Cell Proliferation and Apoptosis during Imaginal Disc Development. [FBrf0167985]

Allele Dmel\slikScer\UAS.cHa

Allele Dmel\slik^Scer\UAS.cHa