FB2025_01 , released February 20, 2025
Allele: Dmel\Oamb286
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General Information
Symbol
Dmel\Oamb286
Species
D. melanogaster
Name
FlyBase ID
FBal0152344
Feature type
allele
Associated gene
Dmel\Oamb
Associated Insertion(s)
P{?lArB}Oamb286
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Lee et al., 2009)
Mutagen
Delta2-3 transposase
(Lee et al., 2003)
Progenitor genotype
P{lArB}OambP5391
(Lee et al., 2003)
OambP5391
(Lee et al., 2003)
Associated Insertion(s)
P{?lArB}Oamb286
Caused by aberration
Df(3R)Oamb286
Cytology
Description

The K3 and AS isoforms of Oamb are disrupted in this mutant.

(Crocker et al., 2010)

Imprecise excision of the P{lArB} element, with one breakpoint in the element and the other downstream of the original insertion site.

(Lee et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The increased amount of sleep normally seen in young flies is still observed in Oamb286 mutants.

      (Kayser et al., 2014)

      Oamb286 mutants exhibit an increase in sleep, which manifests as longer sleep bouts at night and a greater number of sleep bouts during the day.

      Feeding 10 mg/ml octopamine to Oamb286 flies does not produce the decrease in nighttime sleep seen in wild-type controls. Ilp2 neurons in these flies appear to be virtually unresponsive to octopamine, although the Slowpoke current is unaffected.

      (Crocker et al., 2010)

      Homozygous adults have normal gross external morphology.

      (Lee et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Oamb286 is rescued by Scer\GAL4Ilp2.PR/OambUAS.K3

      (Crocker et al., 2010)
      Comments

      Expression of OambScer\UAS.K3 in Scer\GAL4Ilp2.PR neurons results in a significant reduction in sleep in a Oamb286 background, thus effectively rescuing the phenotype. A decrease in nighttime sleep in response to 10 mg/ml octopamine is also rescued in these flies.

      (Crocker et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      OAMB 286
      (Kayser et al., 2014)
      Oamb286
      (Branch et al., 2017, Watanabe et al., 2017)
      oamb286
      (Damrau et al., 2017, Lim et al., 2014, Lee et al., 2009, Lee et al., 2003)
      Name Synonyms
      Secondary FlyBase IDs
        References (9)