Allele Dmel\Oamb²⁸⁶

General Information
Symbol	Dmel\Oamb²⁸⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0152344
Feature type	allele	Associated gene	Dmel\Oamb
Also Known As	oamb²⁸⁶

Allele class	amorphic allele - molecular evidence
Mutagen	Delta2-3
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - molecular evidence (Lee et al., 2009)
Mutagen	Delta2-3 (Lee et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Oamb^P5391 (Lee et al., 2003) P{lArB}Oamb^P5391 (Lee et al., 2003)
Nature of the lesion	Statement Reference The K3 and AS isoforms of Oamb are disrupted in this mutant. (Crocker et al., 2010) Imprecise excision of the P{lArB} element, with one breakpoint in the element and the other downstream of the original insertion site. (Lee et al., 2003)
Caused by insertion	P{?lArB}Oamb²⁸⁶ (Lee et al., 2003, Crocker et al., 2010)
Caused by aberration	Df(3R)Oamb²⁸⁶ (Lee et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	sleep defective (Crocker et al., 2010) viable (Lee et al., 2003)
Phenotype Manifest In

Detailed Description
	Statement Reference Oamb[286] mutants exhibit an increase in sleep, which manifests as longer sleep bouts at night and a greater number of sleep bouts during the day. Feeding 10 mg/ml octopamine to Oamb[286] flies does not produce the decrease in nighttime sleep seen in wild-type controls. Ilp2 neurons in these flies appear to be virtually unresponsive to octopamine, although the Slowpoke current is unaffected. (Crocker et al., 2010) Homozygous adults have normal gross external morphology. (Lee et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Df(3R)6679, Oamb²⁸⁶ has female fertile phenotype (Lee et al., 2003)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Oamb²⁸⁶ is rescued by Scer\GAL4^Ilp2.PR/Oamb^Scer\UAS.K3 (Crocker et al., 2010)
Comments	Expression of Oamb[Scer\UAS.K3] in Scer\GAL4[Ilp2.PR] neurons results in a significant reduction in sleep in a Oamb[286] background, thus effectively rescuing the phenotype. A decrease in nighttime sleep in response to 10 mg/ml octopamine is also rescued in these flies. (Crocker et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	oamb²⁸⁶ (Lee et al., 2003, Lee et al., 2009) Oamb²⁸⁶
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Crocker et al., 2010, Neuron 65(5): 670--681 Identification of a Neural Circuit that Underlies the Effects of Octopamine on Sleep:Wake Behavior. [FBrf0210214] Lee et al., 2009, PLoS ONE 4(3): e4716 The octopamine receptor OAMB mediates ovulation via Ca2+/calmodulin-dependent protein kinase II in the Drosophila oviduct epithelium. [FBrf0207515] Lee et al., 2003, Dev. Biol. 264(1): 179--190 Octopamine receptor OAMB is required for ovulation in Drosophila melanogaster. [FBrf0173197]

Allele Dmel\Oamb286

Allele Dmel\Oamb²⁸⁶