FlyBase Allele Report: Hsap\PRKN[UAS.cYa]

General Information

Symbol

Hsap\PRKN^UAS.cYa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Yang

FlyBase ID

FBal0152456

Feature type

allele

Associated gene

Hsap\PRKN

Associated Insertion(s)

Carried in Construct

P{UAS-hParkin.Y}

Also Known As

UAS-hParkin

Key Links

Transgenic product class

cDNA

(Yang et al., 2003)

wild_type

(Yang et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Yang et al., 2003)

wild_type

(Yang et al., 2003)

Mutagen

in vitro construct

(Yang et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-hParkin.Y}

Cytology

Description

UASt regulatory sequences drive expression of a full length Hsap\PRKN cDNA.

(Yang et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\PRKN

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by park²⁵

(Poole et al., 2008)

modeled by Hsap\LRRK2^I2020T.UAS

(Venderova et al., 2009)

modeled by Hsap\LRRK2^UAS.cVa

(Venderova et al., 2009)

modeled by Hsap\GPR37^UAS.cYa

(Yang et al., 2003)

modeled by Hsap\SNCA^UAS.cFa

(Yang et al., 2003)

modeled by Pink1^RNAi.UAS.cYa

(Yang et al., 2006)

ameliorates neurodegenerative disease

modeled by elgi^UAS.Tag:HA

(Tan et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Expression of Hsap\PARK2^Scer\UAS.cYa results in a complex interaction with the Hsap\LRRK2^Scer\UAS.cVa and Hsap\LRRK2^{I2020T.Scer\UAS} Parkinson's Disease models; some aspects of the eye phenotype (black lesions) are suppressed, whereas others (such as eye pigmentation) are not.

(Venderova et al., 2009)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Phenotype Manifest In

enterocyte | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

eye, with Scer\GAL4^GMR.PF

(Venderova et al., 2009)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Venderova et al., 2009)

midgut | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

mitochondrion | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

pigment cell, with Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Detailed Description

Statement

Reference

The expression of Hsap\PARK2^Scer\UAS.cYa under the control of Scer\GAL4^Act.PU in clones within mosaic midguts results in enterocytes during pupariation exhibiting a significant decrease in the relative mitochondrial content, but no significant changes in the transverse cell area or in the relative amount of autophagosomes, as assessed by a Atg8a fluorescent reporter, as compared to non-clone enterocytes.

(Liu et al., 2016)

Flies expressing Hsap\PARK2^Scer\UAS.cYa under the control of Scer\GAL4^GMR.PF at 29[o]C show a numbers of eye defects including bristle loss, holes and a rough surface.

(Venderova et al., 2009)

Expression of Hsap\PARK2^Scer\UAS.cYa under the control of Scer\GAL4^Ddc.PL does not affect the number of dopaminergic neurons in the dorsomedial clusters in the brain. Expression of Hsap\PARK2^Scer\UAS.cYa under the control of Scer\GAL4^ap-md544 has no effect on adult thoracic morphology.

(Yang et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Hsap\LRRK2^I1122V.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Suppressor of

Statement

Reference

Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A+T453A.UAS.L}

(Wu et al., 2013)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2

(Wu et al., 2013)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^Ddc.PL, elgi^UAS.Tag:HA

(Tan et al., 2011)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor | partially of abnormal flight phenotype of Scer\GAL4^Ddc.PL, elgi^UAS.Tag:HA

(Tan et al., 2011)

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Scer\GAL4^Mef2.PR/Hsap\PRKN^UAS.cYa is a suppressor of abnormal flight phenotype of park²⁵

(Poole et al., 2008)

Scer\GAL4^Mef2.PR/Hsap\PRKN^UAS.cYa is a suppressor of visible phenotype of park²⁵

(Poole et al., 2008)

Scer\GAL4^elav.PLu/Hsap\PRKN^UAS.cYa is a suppressor of abnormal locomotor behavior phenotype of Hsap\GPR37^UAS.cYa

(Umeda-Kameyama et al., 2007)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of abnormal neuroanatomy phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of abnormal neuroanatomy phenotype of Hsap\GPR37^UAS.cYa, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^ap-md544 is a suppressor of visible phenotype of Hsap\GPR37^UAS.cYa, Scer\GAL4^ap-md544

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of abnormal neuroanatomy phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of abnormal neuroanatomy phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Hsap\LRRK2^I1122V.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Enhancer of

Statement

Reference

Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU is an enhancer of midgut | prepupal stage | somatic clone phenotype of Hsap\PINK1^UAS.cYa, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU is an enhancer of enterocyte | prepupal stage | somatic clone phenotype of Hsap\PINK1^UAS.cYa, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU is an enhancer of autophagosome | prepupal stage | somatic clone phenotype of Hsap\PINK1^UAS.cYa, Scer\GAL4^Act.PU

(Liu et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/Hsap\PRKN^UAS.cYa is a suppressor | somatic clone of mitochondrion | somatic clone | prepupal stage phenotype of Pink1^B9

(Liu et al., 2016)

Scer\GAL4^Act.PU/Hsap\PRKN^UAS.cYa is a suppressor | somatic clone of enterocyte | somatic clone | prepupal stage phenotype of Pink1^B9

(Liu et al., 2016)

Scer\GAL4^Act.PU/Hsap\PRKN^UAS.cYa is a suppressor | somatic clone of midgut | somatic clone | prepupal stage phenotype of Pink1^B9

(Liu et al., 2016)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU is a suppressor | somatic clone of mitochondrion | somatic clone | prepupal stage phenotype of Pink1^JF01672, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU is a suppressor | somatic clone of enterocyte | somatic clone | prepupal stage phenotype of Pink1^JF01672, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU is a suppressor | somatic clone of midgut | somatic clone | prepupal stage phenotype of Pink1^JF01672, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2

(Wu et al., 2013)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2

(Wu et al., 2013)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A+T453A.UAS.L}

(Wu et al., 2013)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A+T453A.UAS.L}

(Wu et al., 2013)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor | partially of dopaminergic neuron | adult stage phenotype of Scer\GAL4^Ddc.PL, elgi^UAS.Tag:HA

(Tan et al., 2011)

Hsap\PRKN^UAS.cYa, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Scer\GAL4^Mef2.PR/Hsap\PRKN^UAS.cYa is a suppressor of adult thorax phenotype of park²⁵

(Poole et al., 2008)

Scer\GAL4^elav.PLu/Hsap\PRKN^UAS.cYa is a suppressor of dopaminergic neuron phenotype of Hsap\GPR37^UAS.cYa

(Umeda-Kameyama et al., 2007)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic neuron phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^ap-md544 is a suppressor of adult thorax phenotype of Hsap\GPR37^UAS.cYa, Scer\GAL4^ap-md544

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic neuron phenotype of Hsap\GPR37^UAS.cYa, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic neuron phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic neuron phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The clonal expression of Hsap\PARK2^Scer\UAS.cYa under the control of Scer\GAL4^Act.PU suppresses the increased relative mitochondrial content exhibited during pupariation by enterocytes in Pink1^B9 homozygous midgut clones.

The clonal co-expression of Hsap\PARK2^Scer\UAS.cYa suppresses both the increased relative mitochondrial content and the decreased relative amount of autophagosomes observed during pupariation in enterocytes within midgut clones expressing Pink1^JF01672 under the control of Scer\GAL4^Act.PU, even leading to a significant decrease in relative mitochondrial content and a significant increase in relative amount of autophagosomes, as compared to non-clone enterocytes.

The clonal co-expression of Hsap\PARK2^Scer\UAS.cYa enhances the increased relative amount of autophagosomes exhibited during pupariation by enterocytes in midgut clones expressing Hsap\PINK1^Scer\UAS.cYa under the control of Scer\GAL4^Act.PU.

(Liu et al., 2016)

Expression of Hsap\PARK2^Scer\UAS.cYa completely blocks the ability of rictor^Δ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW.

Expression of Hsap\PARK2^Scer\UAS.cYa completely blocks the ability of trc^{K122A+T453A.Scer\UAS} to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW

(Wu et al., 2013)

The loss of dopaminergic neurons of the adult brain and defects in flight ability caused by expression of Nrdp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Ddc.PL are partially suppressed by co-expression of Hsap\PARK2^Scer\UAS.cYa.

(Tan et al., 2011)

The formation of black lesions in the eye seen in flies expressing Hsap\LRRK2^{I2020T.Scer\UAS} under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of Hsap\PARK2^Scer\UAS.cYa.

(Venderova et al., 2009)

Expression of Hsap\PARK2^Scer\UAS.cYa under the control of Scer\GAL4^Mef2.PR fully rescues the thoracic indentation and flight phenotypes of park²⁵/park²⁵ mutants.

(Poole et al., 2008)

Co-expression of Hsap\PARK2^Scer\UAS.cYa dramatically improves the average climbing activity of 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu.

Co-expression of Hsap\PARK2^Scer\UAS.cYa significantly rescues the reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain seen in 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu.

(Umeda-Kameyama et al., 2007)

Coexpression of Hsap\PARK2^Scer\UAS.cYa suppresses the thoracic degeneration phenotype (leaking body fluid on the dorsal thorax) seen in newly eclosed flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^ap-md544. Coexpression of Hsap\PARK2^Scer\UAS.cYa suppresses the reduction in the number of DA neurons in the DM clusters that is seen in 40 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^Ddc.PL. The reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain seen in 20 day old flies expressing Hsap\SNCA^{A53T.Scer\UAS} under the control of Scer\GAL4^Ddc.PL is suppressed by coexpression of Hsap\PARK2^Scer\UAS.cYa. The reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain seen in 20 day old flies expressing Hsap\SNCA^{A30P.Scer\UAS} under the control of Scer\GAL4^Ddc.PL is suppressed by coexpression of Hsap\PARK2^Scer\UAS.cYa. The reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain seen in 20 day old flies expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^Ddc.PL is suppressed by coexpression of Hsap\PARK2^Scer\UAS.cYa.

(Yang et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hsap\PARK2^Scer\UAS.cYa

Hsap\PRKN^Scer\UAS.cYa

Hsap\PRKN^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Yang

(Yang et al., 2003)

Secondary FlyBase IDs

References (9)

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