Allele Dmel\Hem^{Scer\UAS.T:Myr-Src64B}

General Information
Symbol	Dmel\Hem^{Scer\UAS.T:Myr-Src64B}	Species	D. melanogaster
Name		FlyBase ID	FBal0152477
Feature type	allele	Associated gene	Dmel\Hem

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bogdan and Klambt, 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of the first 88 amino acids of Src64B fused to the full ORF of Hem, is under the control of Scer\UAS sequences. (Bogdan and Klambt, 2003)
Carried in construct	P{UAS-Hem.Myr} (Bogdan et al., 2004, Bogdan and Klambt, 2003, Li and Baker, 2007, Ku et al., 2009, Gault et al., 2012)
Tagged with	T:Myr-Src64B (Bogdan et al., 2004, Bogdan and Klambt, 2003)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^GMR.PU (Ku et al., 2009)
Phenotype Manifest In
	actin filament, with Scer\GAL4^Act5C.PHb (Bogdan and Klambt, 2003) eye, with Scer\GAL4^GMR.PU (Ku et al., 2009) filamentous actin \| pupal stage, with Scer\GAL4^GMR.PU (Ku et al., 2009) larval neuromuscular junction & bouton, with Scer\GAL4^elav-C155 (Bogdan et al., 2004) macrochaeta, with Scer\GAL4^sca-537.4 (Bogdan and Klambt, 2003) microchaeta, with Scer\GAL4^sca.PC (Bogdan et al., 2004) microchaeta \| supernumerary, with Scer\GAL4^sca-537.4 (Bogdan and Klambt, 2003) thecogen cell & actin filament & pupa, with Scer\GAL4^sca-537.4 (Bogdan and Klambt, 2003) thecogen cell \| P-stage, with Scer\GAL4^sca-537.4 (Bogdan and Klambt, 2003)
Detailed Description
	Statement Reference Expression of Hem[Scer\UAS.T:Myr-Src64B] under the control of Scer\GAL4[GMR.PU] results in a rough eye phenotype. 40 hour pupal eye discs show disorganised F-actin patterning compared to wild-type controls. (Ku et al., 2009) Clones of cells expressing Hem^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^Act5C.PP in the wing disc grow equivalently to control clones. 4% of the cells expressing Hem^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^Act5C.PP are apoptotic (compared to 0.2% of control cells) and also 1% of neighbouring cells are apoptotic (compared to 0.3% of cells neighbouring control clones), survival of cells more than one cell diameter away is not affected. 81% of the dying cells adjacent to cells expressing Hem^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^Act5C.PP become engulfed by them (compared to 33% of dying cells engulfed into control cells). (Li and Baker, 2007) Hem^{Scer\UAS.T:Myr1}; Scer\GAL4^sca.PC flies have short, split microchaetae. Neuromuscular junctions in Hem^{Scer\UAS.T:Myr1}; Hem^{Scer\UAS.T:Myr1} third instar larvae exhibit increased budding. (Bogdan et al., 2004) In Hem^{Scer\UAS.T:Myr1}; Scer\GAL4^Act5C.PHb transfected S2 cells F-actin accumulates close to the membrane. Hem^{Scer\UAS.T:Myr1})X3; Scer\GAL4^sca-537.4 flies have shortened, thickened bristles, that are often forked. In addition, epidermal cells in these flies have multiple hairs emerging from each cell. (Hem^{Scer\UAS.T:Myr1})X2; Scer\GAL4^sca-537.4 flies have similar but milder phenotypes. In (Hem^{Scer\UAS.T:Myr1})X3; Scer\GAL4^sca-537.4 pupae, the apical F-actin cytoskeleton of bristle forming (thecogen) cells is disturbed: In contrast to wild type, large and irregular F-actin bundles emerge. In addition, the morphology of thecogen cells abnormal. These cells exhibit more irregular shapes and the cortical F-actin network appears fuzzy. (Bogdan and Klambt, 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Abl^Scer\UAS.cFa, Scer\GAL4^GMR.PU (Ku et al., 2009) Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Ptp61F^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^GMR.PU (Ku et al., 2009)
Suppressed by
Statement Reference Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Abl^{K417N.Scer\UAS}, Scer\GAL4^GMR.PU (Ku et al., 2009) Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Ptp61F^m.Scer\UAS, Scer\GAL4^GMR.PU (Ku et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Abl^Scer\UAS.cFa, Scer\GAL4^GMR.PU (Ku et al., 2009) Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Ptp61F^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^GMR.PU (Ku et al., 2009) Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has filamentous actin \| pupal stage phenotype, enhanceable by Ptp61F^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^GMR.PU (Ku et al., 2009) Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^sca.PC has microchaeta phenotype, enhanceable by Sra-1^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^sca.PC (Bogdan et al., 2004)
Suppressed by
Statement Reference Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Abl^{K417N.Scer\UAS}, Scer\GAL4^GMR.PU (Ku et al., 2009) Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Ptp61F^m.Scer\UAS, Scer\GAL4^GMR.PU (Ku et al., 2009) Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^sca-537.4 has macrochaeta phenotype, suppressible by WASp[+]/WASp³ (Bogdan and Klambt, 2003)
Enhancer of
Statement Reference Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^sca.PC is an enhancer of microchaeta phenotype of Scer\GAL4^sca.PC, Sra-1^{Scer\UAS.T:Myr-Src64B} (Bogdan et al., 2004)
Suppressor of
Statement Reference Hem^{Scer\UAS.T:Myr-Src64B}, Scer\GAL4^sca.PC is a suppressor \| partially of thorax & microchaeta phenotype of Scer\GAL4^sca.PC, Sra-1^{ΔC.Scer\UAS.T:Myr-Src64B} (Bogdan et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference The rough eye phenotype caused by expression of Hem[Scer\UAS.T:Myr-Src64B] under the control of Scer\GAL4[GMR.PU] is enhanced by co-expression of either Ptp61F[dsRNA.Scer\UAS.WIZ] or Abl[Scer\UAS.cFa] and is suppressed by co-expression of either Ptp61F[m.Scer\UAS] or Abl[K417N.Scer\UAS]. The defects seen in F-actin organisation in 40 hour pupal eye discs of animals expressing Hem[Scer\UAS.T:Myr-Src64B] under the control of Scer\GAL4[GMR.PU] are increased significantly by co-expression of Ptp61F[dsRNA.Scer\UAS.WIZ]. (Ku et al., 2009) The short and split microchaetae phenotype of Hem^{Scer\UAS.T:Myr1}; Scer\GAL4^sca.PC flies is synergistically enhanced by Sra-1^{Scer\UAS.T:Myr1}. (Bogdan et al., 2004) The bristle phenotype due to Hem^{Scer\UAS.T:Myr1}; Scer\GAL4^sca-537.4 is dominantly suppressed by WASp³. (Bogdan and Klambt, 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^rho.PL/Hem^{Scer\UAS.T:Myr-Src64B} rescues Hem^C3-20 (Bogdan and Klambt, 2003)
Comments	Hem^{Scer\UAS.T:Myr1}; Scer\GAL4^rho.PL rescues the Hem^C3-20/Hem^C3-20 mutant phenotype in the ventral nerve cord. (Bogdan and Klambt, 2003)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Hem^{Scer\UAS.T:Myr1} Hem^{Scer\UAS.T:Myr-Src64B} Kette^Myr (Bogdan et al., 2004)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Gault et al., 2012, J. Cell Biol. 196(5): 605--621 Drosophila CK1-γ, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. [FBrf0217630] Ku et al., 2009, Mol. Cell. Biol. 29(13): 3623--3632 Organization of F-actin via concerted regulation of Kette by PTP61F and dAbl. [FBrf0215460] Li and Baker, 2007, Cell 129(6): 1215--1225 Engulfment is required for cell competition. [FBrf0201270] Bogdan et al., 2004, Development 131(16): 3981--3989 Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila. [FBrf0179141] Bogdan and Klambt, 2003, Development 130(18): 4427--4437 Kette regulates actin dynamics and genetically interacts with Wave and Wasp. [FBrf0162072]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Allele Dmel\HemScer\UAS.T:Myr-Src64B

Allele Dmel\Hem^{Scer\UAS.T:Myr-Src64B}