FlyBase Allele Report: Dmel\Delta[UAS.cUa]

General Information

Symbol

Dmel\Delta^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0152597

Feature type

allele

Associated gene

Dmel\Delta

Associated Insertion(s)

Carried in Construct

P{UAS-Delta.U}

Also Known As

UAS-Dl, UAS-Delta, UAS-Dl³⁰

Key Links

Transgenic product class

wild_type

(Lunde et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lunde et al., 2003)

Mutagen

in vitro construct

(Lunde et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Delta.U}

Cytology

Description

UAS regulatory sequences drive expression of Dl.

(Lunde et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Delta

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

abnormal size | somatic clone, with Scer\GAL4^Tub.PU

(Herz et al., 2010)

decreased cell number | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

decreased cell number | third instar larval stage, with Scer\GAL4^c768

(Wang et al., 2011)

decreased cell number | third instar larval stage, with Scer\GAL4^C855a

(Wang et al., 2011)

short lived, with Scer\GAL4^ple.PU

(Imai et al., 2015)

visible, with Scer\GAL4^GMR.PF

(Tran et al., 2008)

visible, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

visible, with Scer\GAL4^lz-gal4

(Pepple et al., 2007)

visible | adult stage, with Scer\GAL4^dpp.PU

(Imai et al., 2015)

Phenotype Manifest In

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

dopaminergic PPM1 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

dopaminergic PPM2 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

dopaminergic PPM3 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Tran et al., 2008)

eye | somatic clone, with Scer\GAL4^Tub.PU

(Herz et al., 2010)

interommatidial bristle, with Scer\GAL4^lz-gal4

(Pepple et al., 2007)

larval outer optic anlage, with Scer\GAL4^c768

(Wang et al., 2011)

larval outer optic anlage, with Scer\GAL4^C855a

(Wang et al., 2011)

medulla anlage, with Scer\GAL4^c768

(Wang et al., 2011)

medulla anlage, with Scer\GAL4^C855a

(Wang et al., 2011)

ommatidium, with Scer\GAL4^lz-gal4

(Pepple et al., 2007)

pigment cell, with Scer\GAL4^sv.PJ

(Nagaraj and Banerjee, 2007)

wing | adult stage, with Scer\GAL4^dpp.PU

(Imai et al., 2015)

wing vein L2, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

Detailed Description

Statement

Reference

Expression of Dl^Scer\UAS.cUa under the control of Scer\GAL4^dpp.PU results in shrunken and completely deformed adult wings at 25[o]C but this phenotype is masked at 18[o]C and the wings appear normal.

Expression of Dl^Scer\UAS.cUa under the control of Scer\GAL4^ple.PU (together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly decreased adult lifespan and loss of dopaminergic neurons from the PPL1, PPM1/2 and PPM3 clusters in the adult brain. Expression under the control of Scer\GAL4^elav.PU in combination with Scer\GAL80^ple.PK (and tub-Gal80[ts] to limit the time of expression to adulthood) does not significantly affect adult survival.

(Imai et al., 2015)

Expression of Dl^Scer\UAS.cUa under the control of Scer\GAL4^kirre.PU does not affect myoblast fusion in embryos.

(Tixier et al., 2013)

Expression of Dl^Scer\UAS.cUa under the control of either Scer\GAL4^C855a or Scer\GAL4^c768 results in an expansion of neuroepithelial cells in the outer proliferation center of late-third larval instar optic lobes. Fewer medulla neuroblasts than normal are seen in the mutant brains.

(Wang et al., 2011)

MARCM clones of Dl^Scer\UAS.cUa result in mild overgrowth of the adult eye.

(Herz et al., 2010)

Flies expressing Dl^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF have a rough eye phenotype.

(Tran et al., 2008)

Expression of Dl^Scer\UAS.cUa under the control of Scer\GAL4^sv.PJ results in multiple rows of pigment cells surrounding each ommatidial cluster in the pupal eye disc (in contrast to wild type where each ommatidium is surrounded by a single hexagonal array of pigment cells).

(Nagaraj and Banerjee, 2007)

Expression of Dl^Scer\UAS.cUa under the control of Scer\GAL4^kni.L2 results in modest truncation of wing vein L2 in females and almost complete absence of vein L2 in males.

(Lunde et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Delta^UAS.cUa, Scer\GAL4^ple.PU has decreased cell number | adult stage phenotype, suppressible by neur^UAS.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has abnormal neuroanatomy | adult stage phenotype, suppressible by neur^UAS.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has decreased cell number | adult stage phenotype, suppressible by Lrrk^GD11670, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has abnormal neuroanatomy | adult stage phenotype, suppressible by Lrrk^GD11670, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has decreased cell number | adult stage phenotype, suppressible by Neurl4^GD11930, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has abnormal neuroanatomy | adult stage phenotype, suppressible by Neurl4^GD11930, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has decreased cell number | adult stage phenotype, suppressible by HERC2^VDRC.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has abnormal neuroanatomy | adult stage phenotype, suppressible by HERC2^VDRC.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has abnormal size | somatic clone phenotype, suppressible by Rbf^UAS.cDa, Scer\GAL4^Tub.PU

(Herz et al., 2010)

NOT suppressed by

Statement

Reference

Delta^UAS.cUa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by trbd^Delta/trbd[+]

(Tran et al., 2008)

Suppressor of

Statement

Reference

Delta^UAS.cUa is a suppressor | partially of decreased cell number | larval stage phenotype of Scer\GAL4^GMR9D11, hid^UAS.cUa, rpr^UAS.cUa

(Zhang et al., 2024)

Delta^UAS.cUa, Scer\GAL4^bbg-C96 is a suppressor of visible | adult stage phenotype of Scer\GAL4^bbg-C96, Yif1^GD302

(Kandasamy et al., 2021)

Delta^UAS.cUa, Scer\GAL4^bbg-C96 is a suppressor of decreased size | adult stage phenotype of Scer\GAL4^bbg-C96, Yif1^GD302

(Kandasamy et al., 2021)

Delta^UAS.cUa, Scer\GAL4^lz-gal4 is a suppressor | partially of visible phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Pepple et al., 2007)

Other

Statement

Reference

Delta^UAS.cUa, Hsap\LRRK2^RG.UAS, Scer\GAL4^dpp.PU has visible | adult stage phenotype

(Imai et al., 2015)

Delta^UAS.cUa, Neurl4^EY12221, Scer\GAL4^dpp.PU has visible | adult stage phenotype

(Imai et al., 2015)

Delta^UAS.cUa, HERC2^G17171, Scer\GAL4^dpp.PU has visible | adult stage phenotype

(Imai et al., 2015)

Delta^UAS.cUa, Hsap\LRRK2^UAS.cVa, Scer\GAL4^dpp.PU has visible | adult stage phenotype

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has abnormal size | somatic clone phenotype

(Herz et al., 2010)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has partially lethal - majority die | somatic clone phenotype

(Herz et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM3 neuron | adult stage phenotype, suppressible by neur^UAS.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by Lrrk^GD11670, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM1 neuron | adult stage phenotype, suppressible by Lrrk^GD11670, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM2 neuron | adult stage phenotype, suppressible by Lrrk^GD11670, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM3 neuron | adult stage phenotype, suppressible by Lrrk^GD11670, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by Neurl4^GD11930, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM1 neuron | adult stage phenotype, suppressible by Neurl4^GD11930, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM2 neuron | adult stage phenotype, suppressible by Neurl4^GD11930, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM3 neuron | adult stage phenotype, suppressible by Neurl4^GD11930, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by HERC2^VDRC.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM1 neuron | adult stage phenotype, suppressible by HERC2^VDRC.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM2 neuron | adult stage phenotype, suppressible by HERC2^VDRC.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM3 neuron | adult stage phenotype, suppressible by HERC2^VDRC.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by neur^UAS.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM1 neuron | adult stage phenotype, suppressible by neur^UAS.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^ple.PU has dopaminergic PPM2 neuron | adult stage phenotype, suppressible by neur^UAS.cUa, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has eye | somatic clone phenotype, suppressible by Rbf^UAS.cDa, Scer\GAL4^Tub.PU

(Herz et al., 2010)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has adult head | somatic clone phenotype, suppressible by Rbf^UAS.cDa, Scer\GAL4^Tub.PU

(Herz et al., 2010)

NOT suppressed by

Statement

Reference

Delta^UAS.cUa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by trbd^Delta/trbd[+]

(Tran et al., 2008)

Suppressor of

Statement

Reference

Delta^UAS.cUa is a suppressor | partially of type II neuroblast | larval stage | decreased number phenotype of Scer\GAL4^GMR9D11, hid^UAS.cUa, rpr^UAS.cUa

(Zhang et al., 2024)

Delta^UAS.cUa, Scer\GAL4^bbg-C96 is a suppressor of wing margin phenotype of Scer\GAL4^bbg-C96, Yif1^GD302

(Kandasamy et al., 2021)

Delta^UAS.cUa, Scer\GAL4^lz-gal4 is a suppressor | partially of interommatidial bristle | ectopic phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Pepple et al., 2007)

Other

Statement

Reference

Delta^UAS.cUa, Hsap\LRRK2^RG.UAS, Scer\GAL4^dpp.PU has wing margin phenotype

(Imai et al., 2015)

Delta^UAS.cUa, Neurl4^EY12221, Scer\GAL4^dpp.PU has wing margin phenotype

(Imai et al., 2015)

Delta^UAS.cUa, HERC2^G17171, Scer\GAL4^dpp.PU has wing margin phenotype

(Imai et al., 2015)

Delta^UAS.cUa, Hsap\LRRK2^UAS.cVa, Scer\GAL4^dpp.PU has wing margin phenotype

(Imai et al., 2015)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has eye | somatic clone phenotype

(Herz et al., 2010)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has adult head | somatic clone phenotype

(Herz et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing Dl^Scer\UAS.cUa under the control of Scer\GAL4^dpp.PU and kept at 18[o]C do not display any overt wing phenotype but show notching of the wing margin upon co-expression of either Neurl4^EY12221 or HERC2^G17171.

The loss of dopaminergic neurons from the PPL1, PPM1/2 and PPM3 clusters in the adult brain characteristic for flies expressing Dl^Scer\UAS.cUa under the control of Scer\GAL4^ple.PU (together with tub-Gal80[ts] to limit the time of expression to adulthood) can be prevented by co-expression of any of the following: neur^Scer\UAS.cUa, Lrrk^GD11670, Neurl4^GD11930 or HERC2^VDRC.cUa.

(Imai et al., 2015)

MARCM clones of Dl^Scer\UAS.cUa in Utx¹ mosaic eyes result in massive overgrowth of the adult eye and head. The overgrowth is so strong that only a few mosaic flies are recovered as adults; most die at pupal stages.

Co-expression of Rbf^Scer\UAS.cDa suppresses the strong overgrowth phenotype seen when Dl^Scer\UAS.cUa is overexpressed in Utx¹ mosaic eyes.

(Herz et al., 2010)

trbd^Delta/+ does not suppress the rough eye phenotype caused by expression of Dl^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

(Tran et al., 2008)

Xenogenetic Interactions

Statement

Reference

Flies expressing Dl^Scer\UAS.cUa under the control of Scer\GAL4^dpp.PU and kept at 18[o]C do not display any overt wing phenotype but show notching phenotype upon co-expression of either Hsap\LRRK2^Scer\UAS.cVa or Hsap\LRRK2^RG.Scer\UAS.

(Imai et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Delta^UAS.cUa

Dl^Scer\UAS.cUa

Dl^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

(Lunde et al., 2003)

Secondary FlyBase IDs

References (23)

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