Homozygous embryos cannot form paracellular barriers, show elongated dorsal trunks and show gaps in the ganglionic branches.
Mutants have tracheal defects, including dorsal trunks that are longer than normal and have diameter defects. Lumens are missing in the ganglionic branches. In contrast to wild type, the barrier function has also been lost in the trachea, allowing a dye to penetrate and accumulate in the tracheal lumen.
Unlike wild-type, post stage 15 trachea in AtpαDTS1R2 homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier.