FB2025_01 , released February 20, 2025
Allele: Dmel\sra1
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General Information
Symbol
Dmel\sra1
Species
D. melanogaster
Name
FlyBase ID
FBal0154480
Feature type
allele
Associated gene
Dmel\sra
Associated Insertion(s)
P{lacZ}sra1
Carried in Construct
Also Known As
nla1
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Chang et al., 2003)
Progenitor genotype
Associated Insertion(s)
P{lacZ}sra1
Cytology
Description

P{lacZ} is inserted between the 5' UTR and the translation start site of sra.

(Chang et al., 2003)
Allele components
Component
Use(s)
Inserted element
P{lacZ}
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Down syndrome
      CEA
      (Chang et al., 2003)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Alzheimer's disease
      modeled by Hsap\APP695.UAS.Tag:MYC
      (Shaw and Chang, 2013)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      sra1 flies do not show retinal degeneration even at 45 days old, and third instar larvae do not show significant Syt1 aggregate formation in axons at the NMJ (driven by Scer\GAL4elav.PU), but larvae show significant locomotor defects.

      (Shaw and Chang, 2013)

      Homozygous adults show an increase in the number (12 times wild type) and a decrease in the size (82% reduction) of mitochondria in the photoreceptor axons at the level of the lamina. The morphology of the mitochondria appears normal. The increase in number of the mitochondria is more severe in old flies.

      sra1/sra62 adults show an increase in the number and a decrease in the size of mitochondria in the photoreceptor axons at the level of the lamina.

      Flies show moderate sensitivity to mechanical stress compared to wild type.

      (Chang and Min, 2005)

      sra1 homozygotes show severe learning defects in Pavlovian olfactory conditioning tests. Short term memory appears normal in these flies, but long term memory is absent. In homogenates of whole flies, calcineurin activity is significantly increased and PKA activity is significantly decreased compared to wild-type. Mushroom body morphology is normal in sra1 homozygotes, but.

      (Chang et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      sra1 is an enhancer of retina | adult stage | progressive phenotype of Hsap\APP695.UAS.Tag:MYC, Scer\GAL4GMR.PU

      (Shaw and Chang, 2013)
      NOT Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      sesB1/+ ; sra1 flies are bang sensitive, remaining paralysed for a prolonged period after mechanical stress.

      (Chang and Min, 2005)
      Xenogenetic Interactions
      Statement
      Reference

      sra1 significantly enhances age-dependent retinal degeneration and phototaxis defects in flies with expression of Hsap\APP695.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU.

      sra1 slightly (but not significantly) increases synaptic vesicle accumulation (aggregates) seen in axons at the neuromuscular junction (and does not enhance locomotor defects) in third instar larvae with expression of Hsap\APP695.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4elav.PU. sra1 does not enhance bouton number (synapse proliferation) or satellite bouton number at the NMJ in Scer\GAL4elav.PU>Hsap\APP695.Scer\UAS.T:Hsap\MYC larvae.

      (Shaw and Chang, 2013)
      Complementation and Rescue Data
      Rescued by

      sra1 is rescued by sraUAS.Tag:HA/Scer\GAL4c739

      (Chang et al., 2003)
      Partially rescued by

      sra1 is partially rescued by Scer\GAL4Act5C.PU/sraUAS.Tag:HA

      (Chang and Min, 2005)
      Comments

      Expression of sraScer\UAS.cCa under the control of Scer\GAL4Act5C.PU significantly rescues the increase in number and decrease in size of mitochondria in the photoreceptor axons at the level of the lamina which is seen in sra1 flies.

      (Chang and Min, 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      nla1
      (Shaw and Chang, 2013, Chang and Min, 2005, Chang et al., 2003)
      sra1
      Name Synonyms
      Secondary FlyBase IDs
        References (3)