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General Information
Symbol
Dmel\smoUAS.cHa.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0154569
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of full length smo tagged with Tag:MYC immediately following the putative signal sequence (at the SfiI site at nucleotide 332) and with an AatI site introduced at the end of the 7th transmembrane domain (this introduces the mutations T554S and P555S).
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Overexpression of smoScer\UAS.cHa.T:Hsap\MYC behind the morphogenetic furrow, under the control of Scer\GAL4lz-gal4 generates rough eyes, with defects most pronounced in the posterior. At approximately 24 hours, pupal retinas are relatively normal, although primary cells are often misaligned (27%). By approximately 30 hours, pupal ommatidia exhibit reduced numbers of cone cells (16%) and of lattice cells (75%), with occasional cone cells adopting a rounded morphology characteristic of apoptotic cells. Photoreceptor differentiation, assessed by counting rhabdomeres in pupal retinas, is unaffected in these mutants.
Expression of two copies of smoScer\UAS.cHa.T:Hsap\MYC under the control of hemizygous Scer\GAL4Bx-MS1096 results in the expansion of wing veins L1 and L3 and produces mild hypertrophy of the anterior wing. Expression of high levels of smoScer\UAS.cHa.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 is generally pharate lethal, while adult escapers show transformation of the entire anterior compartment of the wing into vein except for a small strip at the border. The ectopic venation and L3/4 narrowing caused by expression of smoC.Scer\UAS.T:Hsap\MYC,T:Uuuu\Myr4 under the control of Scer\GAL4Bx-MS1096 is suppressed by co-expression of smoScer\UAS.cHa.T:Hsap\MYC.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Wings of flies co-expressing ptcEP941 and high levels of smoScer\UAS.cHa.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 are indistinguishable from those of flies expressing ptcEP941 alone. The reduction in spacing between the 3rd and 4th wing vein seen in flies expressing high levels of fz::smoSSF.Scer\UAS under the control of Scer\GAL4Bx-MS1096 in a smo3/+ background is suppressed by co-expression of smoScer\UAS.cHa.T:Hsap\MYC. The ectopic venation caused by expression of smoScer\UAS.cHa.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 is suppressed by cos+t6.1.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
smoScer\UAS.cHa.T:Hsap\MYC
smoUAS.cHa.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (2)