FlyBase Allele Report: Dmel\psq[rum]

General Information

Symbol

Dmel\psq^rum

Species

D. melanogaster

Name

FlyBase ID

FBal0154668

Feature type

allele

Associated gene

Dmel\psq

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Luschnig et al., 2004, Luschnig et al., 2004)

Progenitor genotype

Cytology

Description

G to A nucleotide substitution affecting the splicing donor site of intron 6, which is common to all known psq transcripts. This leads to partial retention of the intron in the mRNA and is predicted to cause the addition of 16 amino acids followed by a stop codon.

(Grillo et al., 2011)

Nucleotide substitution: G?A.

(Grillo et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:10,612,455..10,612,455 [+]

Nucleotide change:

G10612455A

Reported nucleotide change:

G?A

Comment:

G to A substitution in the splice donor to the third intron in the exons common to all psq isoforms is predicted to lead to translation of an additional 16 amino acids from the intron sequences followed by a stop.

(Grillo et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | germline clone | maternal effect

(Luschnig et al., 2004, Luschnig et al., 2004)

embryo | maternal effect

(Luschnig et al., 2004)

embryonic abdominal segment 8 | maternal effect

(Grillo et al., 2011)

embryonic abdominal segment 8 | maternal effect (with psq⁰¹¹⁵)

(Grillo et al., 2011)

embryonic abdominal segment 8 | maternal effect (with psq^fs1)

(Grillo et al., 2011)

embryonic abdominal segment 8 | maternal effect (with psq^KG02404)

(Grillo et al., 2011)

embryonic abdominal segment 8 | maternal effect (with psq^rev12)

(Grillo et al., 2011)

filzkorper | maternal effect

(Grillo et al., 2011)

filzkorper | maternal effect (with psq⁰¹¹⁵)

(Grillo et al., 2011)

filzkorper | maternal effect (with psq^fs1)

(Grillo et al., 2011)

filzkorper | maternal effect (with psq^KG02404)

(Grillo et al., 2011)

filzkorper | maternal effect (with psq^rev12)

(Grillo et al., 2011)

primordial germ cell | maternal effect

(Grillo et al., 2011)

Detailed Description

Statement

Reference

Homozygous psq^rum mutant flies show no obvious defects, but embryos derived from homozygous psq^rum females display terminal defects.

Embryos derived from psq^fs1/psq^rum mutant females display terminal defects.

Embryos derived from psq⁰¹¹⁵/psq^rum mutant females display terminal defects.

Embryos derived from psq^KG02404/psq^rum mutant females display terminal defects.

Embryos derived from psq^rev12/psq^rum mutant females display terminal defects.

Embryos derived from homozygous psq^rum females display a decreased number of pole cells compared with wild-type controls.

psq^EP2011/psq^rum females are sterile.

psq^KG02404/psq^rum females are sterile.

(Grillo et al., 2011)

Embryos derived from females carrying homozygous germ line clones have strong terminal defects.

(Luschnig et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

psq^rum has embryonic abdominal segment 8 | maternal effect phenotype, non-enhanceable by tor^12D

(Grillo et al., 2011)

psq^rum has filzkorper | maternal effect phenotype, non-enhanceable by tor^12D

(Grillo et al., 2011)

psq^rum has embryonic abdominal segment 8 | maternal effect phenotype, non-enhanceable by cic¹

(Grillo et al., 2011)

psq^rum has filzkorper | maternal effect phenotype, non-enhanceable by cic¹

(Grillo et al., 2011)

Suppressed by

Statement

Reference

psq^rum has embryonic abdominal segment 8 | maternal effect phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/tor^UASp.cGa

(Grillo et al., 2011)

psq^rum has filzkorper | maternal effect phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/tor^UASp.cGa

(Grillo et al., 2011)

NOT suppressed by

Statement

Reference

psq^rum has embryonic abdominal segment 8 | maternal effect phenotype, non-suppressible by tor^12D

(Grillo et al., 2011)

psq^rum has filzkorper | maternal effect phenotype, non-suppressible by tor^12D

(Grillo et al., 2011)

psq^rum has embryonic abdominal segment 8 | maternal effect phenotype, non-suppressible by cic¹

(Grillo et al., 2011)

psq^rum has filzkorper | maternal effect phenotype, non-suppressible by cic¹

(Grillo et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

All embryos from homozygous psq^rum tor^12D double mutant females show terminal defects resembling the psq^rum single mutant phenotype.

Embryos derived from homozygous psq^rum cic¹ double mutant females show terminal defects resembling the psq^rum single mutant phenotype.

Expression of tor^{Scer\UAS.P\T.cGa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the terminal phenotype of psq^rum mutant embryos.

(Grillo et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

psq^rum is rescued by psq^UASp.cGa/Scer\GAL4^{VP16.mat.αTub67C}

(Grillo et al., 2011)

Partially rescued by

psq^rum is partially rescued by Scer\GAL4^{VP16.mat.αTub67C}/psq^UASp.cGb

(Grillo et al., 2011)

Comments

psq^KG02404 fails to complement the female sterility of psq^rum.

psq^EP2011 fails to complement the female sterility of psq^rum.

Expression of psq^{Scer\UAS.P\T.cGa} in the female germ-line under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the psq^rum terminal defects. The majority of the rescued embryos hatch and develop into fertile adults. Pole cell formation is also restored as a result of psq^{Scer\UAS.P\T.cGa}-expression in the germ-line.

Expression of psq^{Scer\UAS.P\T.cGb} in the female germ-line under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} partially rescues the psq^rum terminal defects. Those embryos that display rescued terminal structures hatch, but the others do not. Pole cell formation is restored as a result of psq^{Scer\UAS.P\T.cGb}-expression in the germ-line.

(Grillo et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

92606

y¹ w^*; psq^rum/CyO; P{tor-LacZ.G}3

Notes on Origin

Discoverer

Comments

Complements: cnk^unspecified. Complements: drk^unspecified. Complements: 14-3-3ζ^unspecified. Complements: sag^unspecified. Complements: tor^unspecified.

(Luschnig et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

2R-225-5

(Grillo et al., 2011)

psq^rum

(Grillo et al., 2011)

rum

(Grillo et al., 2011)

rum^2R-225-5

Name Synonyms

rumpf

(Grillo et al., 2011)

Secondary FlyBase IDs

References (3)

Report Sections

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