Allele Dmel\pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC}

General Information
Symbol	Dmel\pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0154740
Feature type	allele	Associated gene	Dmel\pod1

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Rothenberg et al., 2003) in vitro construct - regulatory fusion (Rothenberg et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS sequences drive expression of a pod1 cDNA tagged with T:Avic\GFP and T:Hsap\MYC. (Rothenberg et al., 2003)
Carried in construct	P{UAS-pod1.GFPmyc} (Rothenberg et al., 2003)
Tagged with	T:Avic\GFP (Rothenberg et al., 2003) T:Hsap\MYC (Rothenberg et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective, with Scer\GAL4^Act.PU (Rothenberg et al., 2003)
Phenotype Manifest In
	intersegmental nerve, with Scer\GAL4^elav.PLu (Rothenberg et al., 2003) segmental nerve, with Scer\GAL4^elav.PLu (Rothenberg et al., 2003) ventral nerve cord, with Scer\GAL4^elav.PLu (Rothenberg et al., 2003)
Detailed Description
	Statement Reference When pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC} is driven by Scer\GAL4^elav.PLu multiple, severe axon guidance defects are seen in all axons. 23.3% 44.1% and 56.3% of embryos exhibit defects in the ISN, SNa and ISNb neurons respectively. The ISN exhibits defasciculation, overbranching, and choice point abnormalities. SNa often show missong or misplaced branches, abnormal defasciculation, and defective trajectories. ISNb often frequently arrest and fail to innervate targets in the ventral musculature, remain fasciculated with ISN, or take abnormal trajectories. When pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC} (driven by Scer\GAL4^Act) is over expressed in S2 cells a dose dependent remodelling of cell shape is seen. Cells expressing high levels of pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC} extend long, neurite-like projections that are sometimes branched. These were highly dynamic, displaying behaviours such as growth and extension, lateral movement along the cell surface, retrograde flow, and catastrophic collapse and retraction. These processes are rich in actin bundles. These processes are not dependent on microtubules. (Rothenberg et al., 2003)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC}/Scer\GAL4^elav.PLu partially rescues pod1^Δ96 (Rothenberg et al., 2003)
Comments
Stocks ( 1 )
Bloomington	8800 w^*; P{UAS-pod1.GFPmyc}3/TM3, Sb¹
Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2 cells. (Rothenberg et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Rothenberg et al., 2003, Neuron 39(5): 779--791 Drosophila pod-1 crosslinks both actin and microtubules and controls the targeting of axons. [FBrf0162266]

Allele Dmel\pod1Scer\UAS.T:Avic\GFP,T:Hsap\MYC

Allele Dmel\pod1^{Scer\UAS.T:Avic\GFP,T:Hsap\MYC}