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General Information
Symbol
Dmel\nwkUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Coyle
FlyBase ID
FBal0154814
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full length nwk cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 does not affect synaptic growth at the larval neuromuscular junction (both the average total number of boutons and the number of satellite boutons are normal in the mutant larvae).

Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 has no effect on synaptic growth or bouton number in third instar larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of nwkScer\UAS.cCa with Atg1Scer\UAS.cSa, under the control of Scer\GAL4elav.PLu fails to suppress Atg1Scer\UAS.cSa-mediated neuromuscular junction overgrowth.

Co-expression of nwkScer\UAS.cCa suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in larvae expressing two copies of tkvQ199D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav-C155.

Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in homozygous Dadj1E4 larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Fails to rescue
Comments

Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk1/nwk2 third instar larvae.

The temperature sensitive paralysis phenotype of nwk2 adults is rescued by expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155; after 15 minutes at 28oC, more than 90% of the rescued adults continue to walk and climb normally. Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav.PLu partially rescues the neuromuscular junction (NMJ) phenotypes seen in nwk2 larvae; the normalised numbers of boutons and branches are fully rescued at NMJ 6/7 and NMJ 4, and the increase in NMJ length seen in nwk2 larvae is substantially rescued. The increased incidence of hyperbranched boutons per NMJ seen in nwk2 larvae is not altered in the rescued larvae. Excitatory junctional current (EJC) amplitude is fully rescued and miniature EJC amplitude is partially rescued.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
nwkScer\UAS.cCa
nwkUAS.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Coyle
Secondary FlyBase IDs
    References (4)