|Name||Saccharomyces cerevisiae UAS construct a of Coyle||FlyBase ID||FBal0154814|
|Feature type||allele||Associated gene||Dmel\nwk|
|Mutagen||in vitro construct - regulatory fusion|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Expression of nwk[Scer\UAS.cCa] under the control of Scer\GAL4[elav-C155] does not affect synaptic growth at the larval neuromuscular junction (both the average total number of boutons and the number of satellite boutons are normal in the mutant larvae).
nwkScer\UAS.cCa, Scer\GAL4elav.PLu is a suppressor of increased cell growth phenotype of Atg1Scer\UAS.cSa, Scer\GAL4elav.PLu
nwkScer\UAS.cCa, Scer\GAL4elav.PLu is a suppressor of neuroanatomy defective phenotype of Atg1Scer\UAS.cSa, Scer\GAL4elav.PLu
Scer\GAL4elav-C155/nwkScer\UAS.cCa is a suppressor of neuroanatomy defective | larval stage phenotype of Dadj1E4
|Phenotype Manifest In|
Scer\GAL4elav-C155/nwkScer\UAS.cCa is a suppressor of NMJ bouton | supernumerary | larval stage phenotype of Dadj1E4
|NOT Suppressor of|
nwkScer\UAS.cCa, Scer\GAL4elav.PLu is a non-suppressor of neuromuscular junction phenotype of Atg1Scer\UAS.cSa, Scer\GAL4elav.PLu
Co-expression of nwk[Scer\UAS.cCa] with Atg1[Scer\UAS.cSa], under the control of Scer\GAL4[elav.PLu] fails to suppress Atg1[Scer\UAS.cSa]-mediated neuromuscular junction overgrowth.
Co-expression of nwk[Scer\UAS.cCa] suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in larvae expressing two copies of tkv[Q199D.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav-C155]. Expression of nwk[Scer\UAS.cCa] under the control of Scer\GAL4[elav-C155] suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in homozygous Dad[j1E4] larvae.
|Complementation & Rescue Data|
|Fails to rescue|
Expression of nwk[Scer\UAS.cCa] under the control of Scer\GAL4[elav-C155] partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk/nwk third instar larvae.
The temperature sensitive paralysis phenotype of nwk2 adults is rescued by expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155; after 15 minutes at 28oC, more than 90% of the rescued adults continue to walk and climb normally. Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav.PLu partially rescues the neuromuscular junction (NMJ) phenotypes seen in nwk2 larvae; the normalised numbers of boutons and branches are fully rescued at NMJ 6/7 and NMJ 4, and the increase in NMJ length seen in nwk2 larvae is substantially rescued. The increased incidence of hyperbranched boutons per NMJ seen in nwk2 larvae is not altered in the rescued larvae. Excitatory junctional current (EJC) amplitude is fully rescued and miniature EJC amplitude is partially rescued.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct a of Coyle
|Secondary FlyBase IDs|
|References ( 4 )|