Allele Dmel\nwk^Scer\UAS.cCa

General Information
Symbol	Dmel\nwk^Scer\UAS.cCa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Coyle	FlyBase ID	FBal0154814
Feature type	allele	Associated gene	Dmel\nwk

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Coyle et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a full length nwk cDNA. (Coyle et al., 2004)
Carried in construct	P{UAS-nwk.C} (Coyle et al., 2004, O'Connor-Giles et al., 2008, Shen and Ganetzky, 2009, Rodal et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of nwk[Scer\UAS.cCa] under the control of Scer\GAL4[elav-C155] does not affect synaptic growth at the larval neuromuscular junction (both the average total number of boutons and the number of satellite boutons are normal in the mutant larvae). (O'Connor-Giles et al., 2008) Expression of nwk[Scer\UAS.cCa] under the control of Scer\GAL4[elav-C155] has no effect on synaptic growth or bouton number in third instar larvae. (Rodal et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference nwk^Scer\UAS.cCa, Scer\GAL4^elav.PLu is a suppressor of increased cell growth phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009) nwk^Scer\UAS.cCa, Scer\GAL4^elav.PLu is a suppressor of neuroanatomy defective phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009) Scer\GAL4^elav-C155/nwk^Scer\UAS.cCa is a suppressor of neuroanatomy defective \| larval stage phenotype of Dad^j1E4 (O'Connor-Giles et al., 2008) Scer\GAL4^elav-C155/nwk^Scer\UAS.cCa is a suppressor of neuroanatomy defective \| larval stage phenotype of Scer\GAL4^elav-C155, tkv^{Q199D.Scer\UAS.T:Ivir\HA1} (O'Connor-Giles et al., 2008)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^elav-C155/nwk^Scer\UAS.cCa is a suppressor of NMJ bouton \| supernumerary \| larval stage phenotype of Dad^j1E4 (O'Connor-Giles et al., 2008) Scer\GAL4^elav-C155/nwk^Scer\UAS.cCa is a suppressor of NMJ bouton \| supernumerary \| larval stage phenotype of Scer\GAL4^elav-C155, tkv^{Q199D.Scer\UAS.T:Ivir\HA1} (O'Connor-Giles et al., 2008)
NOT Suppressor of
Statement Reference nwk^Scer\UAS.cCa, Scer\GAL4^elav.PLu is a non-suppressor of neuromuscular junction phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009) nwk^Scer\UAS.cCa, Scer\GAL4^elav.PLu is a non-suppressor of NMJ bouton phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of nwk[Scer\UAS.cCa] with Atg1[Scer\UAS.cSa], under the control of Scer\GAL4[elav.PLu] fails to suppress Atg1[Scer\UAS.cSa]-mediated neuromuscular junction overgrowth. (Shen and Ganetzky, 2009) Co-expression of nwk[Scer\UAS.cCa] suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in larvae expressing two copies of tkv[Q199D.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav-C155]. Expression of nwk[Scer\UAS.cCa] under the control of Scer\GAL4[elav-C155] suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in homozygous Dad[j1E4] larvae. (O'Connor-Giles et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^elav-C155/nwk^Scer\UAS.cCa rescues nwk² (Coyle et al., 2004)
Partially rescues	nwk^Scer\UAS.cCa/Scer\GAL4^elav.PLu partially rescues nwk² (Coyle et al., 2004) nwk^Scer\UAS.cCa partially rescues nwk¹/nwk² (Rodal et al., 2008)
Fails to rescue	nwk^Scer\UAS.cCa/Scer\GAL4^C57 fails to rescue nwk² (Coyle et al., 2004)
Comments	Expression of nwk[Scer\UAS.cCa] under the control of Scer\GAL4[elav-C155] partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk[1]/nwk[2] third instar larvae. (Rodal et al., 2008) The temperature sensitive paralysis phenotype of nwk² adults is rescued by expression of nwk^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155; after 15 minutes at 28^oC, more than 90% of the rescued adults continue to walk and climb normally. Expression of nwk^Scer\UAS.cCa under the control of Scer\GAL4^elav.PLu partially rescues the neuromuscular junction (NMJ) phenotypes seen in nwk² larvae; the normalised numbers of boutons and branches are fully rescued at NMJ 6/7 and NMJ 4, and the increase in NMJ length seen in nwk² larvae is substantially rescued. The increased incidence of hyperbranched boutons per NMJ seen in nwk² larvae is not altered in the rescued larvae. Excitatory junctional current (EJC) amplitude is fully rescued and miniature EJC amplitude is partially rescued. (Coyle et al., 2004)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	nwk^Scer\UAS.cCa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Coyle (Coyle et al., 2004)
Secondary FlyBase IDs

References ( 4 )
Research paper	Shen and Ganetzky, 2009, J. Cell Biol. 187(1): 71--79 Autophagy promotes synapse development in Drosophila. [FBrf0208921] O'Connor-Giles et al., 2008, Neuron 58(4): 507--518 Nervous wreck interacts with thickveins and the endocytic machinery to attenuate retrograde BMP signaling during synaptic growth. [FBrf0204925] Rodal et al., 2008, J. Neurosci. 28(33): 8316--8325 Nervous wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth. [FBrf0205746] Coyle et al., 2004, Neuron 41(4): 521--534 Nervous wreck, an SH3 adaptor protein that interacts with Wsp, regulates synaptic growth in Drosophila. [FBrf0175021]

Allele Dmel\nwkScer\UAS.cCa

Allele Dmel\nwk^Scer\UAS.cCa