FlyBase Allele Report: Dmel\nwk[1]

General Information

Symbol

Dmel\nwk¹

Species

D. melanogaster

Name

FlyBase ID

FBal0154819

Feature type

allele

Associated gene

Dmel\nwk

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Coyle et al., 2004)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Coyle et al., 2004)

abnormal neuroanatomy (with nwk²)

(Kelley et al., 2015)

abnormal neuroanatomy | larval stage (with nwk²)

(O'Connor-Giles et al., 2008)

abnormal neuroanatomy | third instar larval stage

(Rodal et al., 2008)

abnormal neuroanatomy | third instar larval stage (with nwk²)

(Rodal et al., 2008)

abnormal neuroanatomy | third instar larval stage (with nwk²), with Scer\GAL4^elav-C155, nwk^SH3ab.UAS

(Rodal et al., 2008)

abnormal neurophysiology

(Coyle et al., 2004)

abnormal size | third instar larval stage

(Rodal et al., 2008)

paralytic | heat sensitive

(Coyle et al., 2004)

paralytic | heat sensitive (with nwk²)

(Coyle et al., 2004)

paralytic | heat sensitive (with nwk³)

(Coyle et al., 2004)

paralytic | heat sensitive (with nwk⁴)

(Coyle et al., 2004)

paralytic | heat sensitive (with nwk⁵)

(Coyle et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

nwk¹/nwk² mutant third instar larvae show an increase in the total synaptic bouton number at the neuromuscular junction as well as an increase in the satellite boutons.

(Kelley et al., 2015)

nwk¹ mutant third instar larvae exhibit enlarged neuromuscular junctions.

(Khuong et al., 2010)

Total bouton number and satellite bouton number at the neuromuscular junction is increased in nwk¹/nwk² larvae compared to controls.

(O'Connor-Giles et al., 2008)

nwk¹ mutants display synaptic overgrowth. The mean bouton number for segment A3, muscle 6/7 is increased compared to wild type and a greater number of satellite boutons is seen in segments A2-A3, muscle 4.

nwk¹/nwk² mutants exhibit an increase in overall bouton number.

Expression of nwk^{SH3ab.Scer\UAS} under the control of Scer\GAL4^elav-C155 enhances the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

(Rodal et al., 2008)

Mutant adults behave normally at room temperature, but lose coordination within 10-40 seconds at 38^oC and undergo seizure-like spasms that gradually diminish until the flies become paralysed within 3 minutes. After a 5 minutes exposure to 38^oC, the mutant flies gradually recover when returned to room temperature, and normal behaviour is fully restored after 5-10 minutes. The number of boutons at neuromuscular junction (NMJ) 6/7 is increased by about 50% in mutant larvae compared to controls and a similar increase in bouton number is also seen at NMJ 4. When normalised to muscle surface area, the bouton number at NMJ 6/7 is about 50% larger than in controls, while at NMJ 4 the bouton number is 22% larger than in controls. The NMJ length/muscle area is increased by about 30% compared to controls. The proportion of branching boutons is increased by 50% at NMJ 6/7 and by 125% at NMJ 4 in mutant larvae compared to controls. There is also a striking increase in the incidence of hyperbranched boutons (those extending 3 or more new branches) compared to wild type; at least one is seen in all NMJs 6/7 and about half of all NMJs 4 in the mutant larvae. Most of these hyperbranched boutons extend 3 new branches, but about 10% extend 4. The mean amplitude of the excitatory junctional current (EJC) at neuromuscular junction 6/7 is reduced by more than 50% compared to wild-type larvae. The amplitude of miniature EJCs (quantal size) is slightly increased and mEJC frequency is slightly elevated compared to wild type.

(Coyle et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, enhanceable by EndoA^EY02730/endoA[+]

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, enhanceable by Dap160[+]/Dap160^Δ1

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, enhanceable by shi[+]/shi¹

(O'Connor-Giles et al., 2008)

nwk¹ has abnormal size | third instar larval stage phenotype, enhanceable by Cdc42²

(Rodal et al., 2008)

nwk¹ has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Cdc42²

(Rodal et al., 2008)

Suppressed by

Statement

Reference

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, suppressible by wit^A12/wit[+]

(O'Connor-Giles et al., 2008)

nwk¹ has abnormal size | third instar larval stage phenotype, suppressible by wit^B11/wit^A12

(Rodal et al., 2008)

nwk¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wit^B11/wit^A12

(Rodal et al., 2008)

Enhancer of

Statement

Reference

nwk¹ is an enhancer of abnormal size | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹ is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹/nwk² is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

NOT Suppressor of

Statement

Reference

nwk¹ is a non-suppressor of abnormal size | third instar larval stage phenotype of wit^A12

(Rodal et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

nwk¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}

(Khuong et al., 2010)

nwk¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by Df(3R)3450/WASp¹

(Khuong et al., 2010)

nwk¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by Scer\GAL4^nSyb.PS/tkv^{Q253D.UAS.cNb}

(Khuong et al., 2010)

nwk¹/nwk² has NMJ bouton | larval stage | increased number phenotype, enhanceable by EndoA^EY02730/endoA[+]

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has NMJ bouton | larval stage | increased number phenotype, enhanceable by Dap160[+]/Dap160^Δ1

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has NMJ bouton | larval stage | increased number phenotype, enhanceable by shi[+]/shi¹

(O'Connor-Giles et al., 2008)

nwk¹ has NMJ bouton | third instar larval stage phenotype, enhanceable by wit^B11/wit^A12

(Rodal et al., 2008)

nwk¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by Cdc42²

(Rodal et al., 2008)

NOT Enhanced by

Statement

Reference

Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}, Scer\GAL4^nSyb.PS, nwk¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Df(3R)3450/WASp¹

(Khuong et al., 2010)

Suppressed by

Statement

Reference

nwk¹/nwk² has NMJ bouton | increased number | larval stage phenotype, suppressible by wit^A12/wit[+]

(O'Connor-Giles et al., 2008)

nwk¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by wit^B11/wit^A12

(Rodal et al., 2008)

nwk¹ has NMJ bouton | third instar larval stage phenotype, suppressible by Cdc42²

(Rodal et al., 2008)

Enhancer of

Statement

Reference

nwk¹ is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Df(3R)3450/WASp¹

(Khuong et al., 2010)

nwk¹ is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹ is an enhancer of NMJ bouton | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹/nwk² is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹/nwk² is an enhancer of NMJ bouton | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

NOT Enhancer of

Statement

Reference

nwk¹ is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage | somatic clone phenotype of tweek²/tweek¹

(Khuong et al., 2010)

NOT Suppressor of

Statement

Reference

nwk¹ is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of wit^A12

(Rodal et al., 2008)

nwk¹ is a non-suppressor of NMJ bouton | third instar larval stage phenotype of wit^A12

(Rodal et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

nwk¹ enhances the overgrown neuromuscular junction phenotype seen in WASp¹/Df(3R)3450 third instar larvae.

WASp¹/Df(3R)3450 enhances the overgrown neuromuscular junction phenotype seen in homozygous nwk¹ third instar larvae.

Expression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^n-syb.PS enhances the neuromuscular junction overgrowth phenotype seen in nwk¹ third instar larvae.

nwk¹ does not enhance the neuromuscular junction overgrowth phenotype seen in tweek¹/tweek² third instar larvae.

(Khuong et al., 2010)

The increase in total bouton number and satellite bouton number at the neuromuscular junction that is seen in nwk¹/nwk² larvae is enhanced if the larvae are also carrying endoA^EY02730/+, Dap160^Δ1/+ or shi¹/+.

wit^A12/+ suppresses the increase in total bouton number at the neuromuscular junction that is seen in nwk¹/nwk² larvae.

(O'Connor-Giles et al., 2008)

A wit^A12/wit^B11 transheterozygous background suppresses the synaptic overgrowth and increased bouton number phenotypes found in nwk¹ homozygous third instar larvae, instead the larvae exhibit reduced synaptic growth and fewer boutons than wild type controls.

Cdc42² nwk¹ double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant.

(Rodal et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS enhances the overgrown neuromuscular junction phenotype seen in nwk¹ third instar larvae.

Expression of WASp¹/Df(3R)3450 does not enhance the enlarged neuromuscular junction phenotype seen when Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^n-syb.PS in homozygous nwk¹ third instar larvae.

(Khuong et al., 2010)

Complementation and Rescue Data

Fails to complement

nwk²

(Coyle et al., 2004)

nwk³

(Coyle et al., 2004)

nwk⁴

(Coyle et al., 2004)

nwk⁵

(Coyle et al., 2004)

Rescued by

nwk¹/nwk² is rescued by Scer\GAL4^elav-C155/nwk^UAS.EGFP

(Kelley et al., 2015)

Partially rescued by

nwk¹/nwk² is partially rescued by nwk^UAS.cCa

(Rodal et al., 2008)

nwk¹/nwk² is partially rescued by Scer\GAL4^elav-C155/nwk^ΔC.UAS

(Rodal et al., 2008)

Not rescued by

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^{Δ1-428.UAS.EGFP}

(Kelley et al., 2015)

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^{1-428.UAS.EGFP}

(Kelley et al., 2015)

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^SH3a.UAS

(Rodal et al., 2008)

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^SH3b.UAS

(Rodal et al., 2008)

nwk¹/nwk² is not rescued by nwk^SH3ab.UAS

(Rodal et al., 2008)

Comments

The increased number of boutons at the neuromuscular junction observed in the nwk¹/nwk² mutant third instar larvae is rescued by expression of nwk^{Scer\UAS.T:Avic\GFP-EGFP}, is not changed by expression of nwk^{Δ1-428.Scer\UAS.T:Avic\GFP-EGFP} and is increased further by expression of nwk^{1-428.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav-C155 in the mutant background.

(Kelley et al., 2015)

Expression of nwk^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

Expression of nwk^{SH3a.Scer\UAS} under the control of Scer\GAL4^elav-C155 fails to rescue the synaptic overgrowth and increased bouton number phenotypes in nwk¹/nwk² third instar larvae.

Expression of nwk^{SH3b.Scer\UAS} under the control of Scer\GAL4^elav-C155 fails to rescue the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

Expression of nwk^{SH3ab.Scer\UAS} under the control of Scer\GAL4^elav-C155 enhances the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

Expression of nwk^ΔC.Scer\UAS under the control of Scer\GAL4^elav-C155 partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

(Rodal et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51626

nwk¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nwk

(Coyle et al., 2004)

nwk¹

(Del Signore et al., 2023, Del Signore et al., 2021, Stanishneva-Konovalova et al., 2016, Sulkowski et al., 2016, Kelley et al., 2015, Khuong et al., 2010, O'Connor-Giles et al., 2008, Rodal et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close