FlyBase Allele Report: Dmel\kek1[UAS.GFP]

General Information

Symbol

Dmel\kek1^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0155160

Feature type

allele

Associated gene

Dmel\kek1

Associated Insertion(s)

Carried in Construct

P{UAS-kek1.GFP}

Key Links

Transgenic product class

wild_type

(Alvarado et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Alvarado et al., 2004)

Mutagen

in vitro construct

(Alvarado et al., 2004, Alvarado et al., 2004, Derheimer et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-kek1.GFP}

Cytology

Description

UASt sequences drive expression of kek1 tagged with GFP.

(Alvarado et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

kek1

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PI

(Alvarado et al., 2004)

visible, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

Phenotype Manifest In

egg chorion, with Scer\GAL4^CY2

(Alvarado et al., 2004)

egg chorion | dorsal, with Scer\GAL4^CY2

(Alvarado et al., 2004, Derheimer et al., 2004)

eye, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004, Alvarado et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

Detailed Description

Statement

Reference

When kek1^{Scer\UAS.T:Avic\GFP} is driven by Scer\GAL4^GMR.PF, animals display a severe rough eye phenotype. When driven by Scer\GAL4^CY2 a strongly ventralised phenotype is seen in the chorion.

(Alvarado et al., 2004)

Expression of kek1^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^CY2 during oogenesis results in eggs with strongly ventralised chorions. Expression of kek1^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Alvarado et al., 2004)

When driven by Scer\GAL4^CY2 a strongly ventralised phenotype is seen in the chorion.

(Derheimer et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S3 cells for C-immunoprecipitation experiments.

(Alvarado et al., 2004, Derheimer et al., 2004)

Carried in a plasmid and transfected into S3 cells for co-immunoprecipitation experiments.

(Alvarado et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

kek1^{Scer\UAS.T:Avic\GFP}

kek1^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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