Presynaptic expression of BsgScer\UAS.cBa (under the control of Scer\GAL4elav-C155 in a BsgNP6293/Df(2L)Exel7034 mutant background rescues both the asynchronous evoked release and the high frequency of spontaneous release. Presynaptic expression decreases the amplitude of eEJCs and the frequency of single vesicle release below control levels, indicating a dose-dependent role. Expression post-synaptically, under the control of Scer\GAL4Mhc.PW does not rescue.
Expression of wild-type BsgScer\UAS.cBa solely in muscles (using Scer\GAL4Mhc.PW or Scer\GAL4how-24B) or in neurons (using Scer\GAL4elav-C155) partially, but significantly, rescued both the increase in bouton size and the reduction of bouton number observed in BsgNP6293/Df(2L)Exel7034 mutant larvae. Near-complete rescue of bouton size and junction growth is obtained upon expression of BsgScer\UAS.cBa both pre- and postsynaptically (using Scer\GAL4Mhc.PW or Scer\GAL4how-24B post-synaptically and Scer\GAL4elav-C155 pre-synaptically).