FlyBase Allele Report: Dmel\brat[2L-192-9]

General Information

Symbol

Dmel\brat^2L-192-9

Species

D. melanogaster

Name

FlyBase ID

FBal0155551

Feature type

allele

Associated gene

Dmel\brat

Associated Insertion(s)

Carried in Construct

Also Known As

brat¹⁹²

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Shi et al., 2013)

Mutagen

ethyl methanesulfonate

(Luschnig et al., 2004, Luschnig et al., 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q417term.

(Betschinger et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:19,169,394..19,169,394 [+]

Nucleotide change:

C19169394T

Amino acid change:

Reported amino acid change:

Q417term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Betschinger et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Betschinger et al., 2006)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with brat^2L-150-11)

(Shi et al., 2013)

abnormal neuroanatomy | third instar larval stage (with brat¹¹)

(Shi et al., 2013)

abnormal neuroanatomy | third instar larval stage (with Df(2L)pr-A16)

(Shi et al., 2013)

abnormal neurophysiology | third instar larval stage (with brat^2L-150-11)

(Shi et al., 2013)

abnormal neurophysiology | third instar larval stage (with brat¹¹)

(Shi et al., 2013)

increased cell number | somatic clone | third instar larval stage

(Betschinger et al., 2006)

increased occurrence of cell division | somatic clone | third instar larval stage

(Betschinger et al., 2006)

lethal - all die during embryonic stage | germline clone | maternal effect | recessive

(Luschnig et al., 2004)

lethal - all die during embryonic stage | germline clone | non-rescuable maternal effect | recessive

(Luschnig et al., 2004)

neoplasia | somatic clone

(Betschinger et al., 2006)

some die during embryonic stage | maternal effect | recessive

(Luschnig et al., 2004)

some die during embryonic stage | non-rescuable maternal effect | recessive

(Luschnig et al., 2004)

Phenotype Manifest In

axon | somatic clone

(Betschinger et al., 2006)

embryo | germline clone | maternal effect | posterior

(Luschnig et al., 2004)

neuroblast | somatic clone

(Betschinger et al., 2006)

NMJ bouton | increased number | third instar larval stage (with brat^2L-150-11)

(Shi et al., 2013)

NMJ bouton | increased number | third instar larval stage (with brat¹¹)

(Shi et al., 2013)

NMJ bouton | increased number | third instar larval stage (with Df(2L)pr-A16)

(Shi et al., 2013)

postsynaptic density (with brat¹¹)

(Shi et al., 2013)

presynaptic active zone membrane (with brat¹¹)

(Shi et al., 2013)

synaptic vesicle (with brat¹¹)

(Shi et al., 2013)

Detailed Description

Statement

Reference

brat¹¹/brat^2L-192-9 larvae show an increase in total bouton number and in number of satellite boutons at the neuromuscular junction compared to wild type. Ultrastructurally, the presynaptic mitochondria and postsynaptic subsynaptic reticulum appear largely normal in the mutant boutons. However, vesicle density within the bouton is moderately, but significantly, reduced and a subpopulation of vesicles are larger than in wild type. There are fewer synaptic vesicles within a 200nm radius of the T-bar than normal, and the mean synaptic vesicle diameter within the active zone is significantly increased. There is a significant increase in the number of presynaptic membrane ruffles within the active zone and the mean length of the postsynaptic density is increased.

brat^2L-150-11/brat^2L-192-9 larvae show an increase in total bouton number and in number of satellite boutons at the neuromuscular junction compared to wild type.

brat^2L-192-9/Df(2L)pr-A16 larvae show an increase in number of satellite boutons at the neuromuscular junction compared to wild type.

The mean EJP amplitude and mEJP frequency is not significantly different from wild type at the neuromuscular junction of brat¹¹/brat^2L-192-9 and brat^2L-150-11/brat^2L-192-9 larvae. However, in both cases, mean mEJP amplitude is increased compared to wild type. Quantal content is significantly reduced in brat¹¹/brat^2L-192-9 larvae.

The neuromuscular junctions of brat¹¹/brat^2L-192-9 larvae show reduced uptake of FM1-43 dye compared to wild type, indicating a defect in endocytosis.

The neuromuscular junctions of brat¹¹/brat^2L-192-9 and brat^2L-150-11/brat^2L-192-9 larvae show reduced uptake of FM1-43 dye compared to wild type, indicating a defect in endocytosis.

(Shi et al., 2013)

Mutant germline clones do not show any obvious defects in oogenesis.

(Neumuller et al., 2008)

brat^2L-192-9 neuroblast clones are larger than wild-type clones and have a significantly increased mitotic index. No axons can be observed extending from daughter cells in these clones.

(Betschinger et al., 2006)

Embryos derived from females carrying homozygous germ line clones have posterior defects.

(Luschnig et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

brat^2L-192-9/brat¹¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Mad¹²/Mad[+]

(Shi et al., 2013)

brat^2L-192-9/brat¹¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Df(2L)BSC162/+

(Shi et al., 2013)

NOT suppressed by

Statement

Reference

brat^2L-192-9/brat¹¹ has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by wit^A12/wit[+]

(Shi et al., 2013)

brat^2L-192-9/brat¹¹ has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by tkv⁷/tkv[+]

(Shi et al., 2013)

Other

Statement

Reference

Dad^j1E4/Dad[+], brat^2L-192-9 has abnormal neuroanatomy | third instar larval stage phenotype

(Shi et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

brat^2L-192-9/brat¹¹ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Mad¹²/Mad[+]

(Shi et al., 2013)

brat^2L-192-9/brat¹¹ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Df(2L)BSC162/+

(Shi et al., 2013)

NOT suppressed by

Statement

Reference

brat^2L-192-9/brat¹¹ has NMJ bouton | increased number | third instar larval stage phenotype, non-suppressible by wit^A12/wit[+]

(Shi et al., 2013)

brat^2L-192-9/brat¹¹ has NMJ bouton | increased number | third instar larval stage phenotype, non-suppressible by tkv⁷/tkv[+]

(Shi et al., 2013)

Other

Statement

Reference

Dad^j1E4/Dad[+], brat^2L-192-9 has NMJ bouton | increased number | third instar larval stage phenotype

(Shi et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The neuromuscular junction overgrowth phenotype (increased total number of boutons and increased number of satellite boutons) of brat¹¹/brat^2L-192-9 larvae is not suppressed by either wit^A12/+ or tkv⁷/+.

The neuromuscular junction overgrowth phenotype (increased total number of boutons and increased number of satellite boutons) of brat¹¹/brat^2L-192-9 larvae is significantly suppressed by Mad¹²/+ and by Df(2L)BSC162/+.

brat^2L-192-9/+ ; Dad^j1E4/+ double heterozygotes show a significant increase in the number of satellite boutons at the larval neuromuscular junction compared to wild type.

The reduced uptake of FM1-43 dye seen at the neuromuscular junction in brat¹¹/brat^2L-192-9 larvae is significantly suppressed by Mad¹²/+ and by Df(2L)BSC162/+.

(Shi et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

brat^2L-192-9/brat¹¹ is partially rescued by Scer\GAL4^RapGAP1-OK6/brat^UAS.Tag:FLAG

(Shi et al., 2013)

brat^2L-192-9/brat¹¹ is partially rescued by Scer\GAL4^elav.PU/brat^UAS.Tag:FLAG

(Shi et al., 2013)

Not rescued by

brat^2L-192-9/brat¹¹ is not rescued by brat^UAS.Tag:FLAG/Scer\GAL4^Mhc.PW

(Shi et al., 2013)

Comments

Expression of brat^{Scer\UAS.T:Zzzz\FLAG} under the control of either Scer\GAL4^elav.PU or Scer\GAL4^RapGAP1-OK6 at 18[o]C partially rescues the increase in number of satellite boutons at the neuromuscular junction (NMJ) seen in brat¹¹/brat^2L-192-9 larvae. Expression under the control of Scer\GAL4^Mhc.PW at 18[o]C does not rescue the NMJ phenotype.

Expression of brat^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU at 18[o]C partially rescues the endocytotic defects seen at the NMJ of brat¹¹/brat^2L-192-9 larvae.

(Shi et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

2L-192-9

(Luschnig et al., 2004)

brat¹⁹²

(Marchetti et al., 2014, Shi et al., 2013, Neumuller et al., 2008, Betschinger et al., 2006)

brat^2L-192-9

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close