Allele Dmel\Sp1^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Sp1^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0155796
Feature type	allele	Associated gene	Dmel\Sp1

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Estella et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of a 813 fragment of the Sp1 gene inserted as an inverted repeat. (Estella et al., 2003)
Carried in construct	P{UAS-Sp1-RNAi} (Estella et al., 2003, Estella and Mann, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	size defective, with Scer\GAL4^btd-MD808, Scer\GAL80^ts.αTub84B, Sp1^GD4097 (Estella and Mann, 2010) size defective, with Scer\GAL4^btd-MD808, Sp1^GD4097 (Estella and Mann, 2010) size defective, with Scer\GAL4^dac-p7d23, Scer\GAL80^ts.αTub84B, Sp1^GD4097 (Estella and Mann, 2010) size defective, with Scer\GAL4^Dll-em212, Scer\GAL80^ts.αTub84B, Sp1^GD4097 (Estella and Mann, 2010)
Phenotype Manifest In
	first segment of antenna, with Scer\GAL4^btd-MD808, Scer\GAL80^ts.αTub84B, Sp1^GD4097 (Estella and Mann, 2010) leg, with Scer\GAL4^btd-MD808, Sp1^GD4097 (Estella and Mann, 2010) leg \| distal, with Scer\GAL4^Dll-em212, Scer\GAL80^ts.αTub84B, Sp1^GD4097 (Estella and Mann, 2010) leg \| medial, with Scer\GAL4^dac-p7d23, Scer\GAL80^ts.αTub84B, Sp1^GD4097 (Estella and Mann, 2010) second segment of antenna, with Scer\GAL4^btd-MD808, Scer\GAL80^ts.αTub84B, Sp1^GD4097 (Estella and Mann, 2010)
Detailed Description
	Statement Reference Co-expression of Sp1[dsRNA.Scer\UAS], Sp1[GD4097] and Dcr-2[Scer\UAS.cDa] under the control of Scer\GAL4[dac-p7d23] (restricted to second instar stage onwards using Scer\GAL80[ts.αTub84B]) results in a strong reduction in the size of the medial part of the leg. Co-expression of Sp1[dsRNA.Scer\UAS], Sp1[GD4097] and Dcr-2[Scer\UAS.cDa] under the control of Scer\GAL4[Dll-em212] (restricted to second instar stage onwards using Scer\GAL80[ts.αTub84B]) results in a strong reduction in the size of the distal part of the leg. Co-expression of Sp1[dsRNA.Scer\UAS], Sp1[GD4097] and Dcr-2[Scer\UAS.cDa] under control of Scer\GAL4[btd-MD808] (restricted to second instar stage onwards using Scer\GAL80[ts.αTub84B]) results in loss of the antennal a1 and a2 segments. Co-expression of Sp1[dsRNA.Scer\UAS], Sp1[GD4097] and Dcr-2[Scer\UAS.cDa] under control of Scer\GAL4[btd-MD808] from embryogenesis onwards results in a reduction in the size of the entire leg, from coxa to the distal tip. (Estella and Mann, 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement Reference Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808, btd^{dsRNA.Scer\UAS} has first segment of antenna \| heat sensitive phenotype (Estella et al., 2003, Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808, btd^{dsRNA.Scer\UAS} has leg \| heat sensitive phenotype (Estella et al., 2003, Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808, btd^{dsRNA.Scer\UAS} has second segment of antenna \| heat sensitive phenotype (Estella et al., 2003, Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808, btd^{dsRNA.Scer\UAS} has tarsal segment \| heat sensitive phenotype (Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808, btd^{dsRNA.Scer\UAS} has tarsal segment phenotype (Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808, btd^{dsRNA.Scer\UAS} has third segment of antenna \| heat sensitive phenotype (Estella et al., 2003, Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808/btd^{dsRNA.Scer\UAS} has first segment of antenna \| heat sensitive phenotype (Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808/btd^{dsRNA.Scer\UAS} has leg \| heat sensitive phenotype (Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808/btd^{dsRNA.Scer\UAS} has second segment of antenna \| heat sensitive phenotype (Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808/btd^{dsRNA.Scer\UAS} has tarsal segment \| heat sensitive phenotype (Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808/btd^{dsRNA.Scer\UAS} has third segment of antenna \| heat sensitive phenotype (Estella et al., 2003) Scer\GAL4^btd-MD808, Sp1^{dsRNA.Scer\UAS}, btd^MD808 has tarsal segment phenotype (Estella et al., 2003, Estella et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference When Sp1^{dsRNA.Scer\UAS} is expressed in a btd^MD808 background (driven by the Scer\GAL4^btd-MD808 aspect of the P{GawB}btd^MD808 insertion), at most some reduction in the tarsus is seen in some mutant legs. When both btd^{dsRNA.Scer\UAS} and Sp1^{dsRNA.Scer\UAS} are expressed in a btd^MD808 background (at 29^oC) and driven by the Scer\GAL4^btd-MD808 aspect of the P{GawB}btd^MD808 insertion), the animals are unable to hatch and the antennae and legs are affected in all cases, though to different extents. Segments I and II of the tarsus are often reduced. Leg segments are reduced in size and often fused. When these flies are grown at 17^oC almost no alteration is seen. (Estella et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Sp1^{dsRNA.Scer\UAS} Sp1^{Scer\UAS.dsRNA}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Estella and Mann, 2010, PLoS Genet. 6(6): e1001001 Non-Redundant Selector and Growth-Promoting Functions of Two Sister Genes, buttonhead and Sp1, in Drosophila Leg Development. [FBrf0211142] Estella et al., 2003, Development 130(24): 5929--5941 The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila. [FBrf0167505]

Allele Dmel\Sp1dsRNA.Scer\UAS

Allele Dmel\Sp1^{dsRNA.Scer\UAS}