Allele Dmel\Socs36E^{Scer\UAS.P\T.cCa}

General Information
Symbol	Dmel\Socs36E^{Scer\UAS.P\T.cCa}	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Callus	FlyBase ID	FBal0155806
Feature type	allele	Associated gene	Dmel\Socs36E

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bach et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of Socs36E. (Bach et al., 2003) Scer\UAS regulatory and P\T promoter sequences drive expression of Socs36E. (Callus and Mathey-Prevot, 2002)
Carried in construct	P{UASp-Socs36E.C} (Silver et al., 2005, Callus and Mathey-Prevot, 2002, Bach et al., 2003, Almudi et al., 2009, Bruckner et al., 2004, Terry et al., 2006, Wawersik et al., 2005, Issigonis et al., 2009, Almudi et al., 2010, Singh et al., 2010, Copf et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| partially \| pharate adult stage, with Scer\GAL4^en-e16E (Callus and Mathey-Prevot, 2002) memory defective \| adult stage \| RU486 conditional, with Scer\GAL4^{Mef2.247.Switch} (Copf et al., 2011) short lived, with Scer\GAL4^Act5C.PI (Callus and Mathey-Prevot, 2002) visible, with Scer\GAL4^ap-md544 (Callus and Mathey-Prevot, 2002) visible, with Scer\GAL4^en-e16E (Callus and Mathey-Prevot, 2002) visible, with Scer\GAL4^hs.2sev (Almudi et al., 2009)
Phenotype Manifest In
	anterior crossvein, with Scer\GAL4^en-e16E (Callus and Mathey-Prevot, 2002) border follicle cell, with Scer\GAL4^c306 (Silver et al., 2005) border follicle cell, with Scer\GAL4^slbo.2.6 (Silver et al., 2005) germline cell \| embryonic stage \| male, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Wawersik et al., 2005) haltere, with Scer\GAL4^en-e16E (Callus and Mathey-Prevot, 2002) humerus, with Scer\GAL4^en-e16E (Callus and Mathey-Prevot, 2002) photoreceptor cell R7, with Scer\GAL4^hs.2sev (Almudi et al., 2009) pigment cell, with Scer\GAL4^en-e16E (Callus and Mathey-Prevot, 2002) wing, with Scer\GAL4^Act5C.PI (Callus and Mathey-Prevot, 2002) wing, with Scer\GAL4^ap-md544 (Callus and Mathey-Prevot, 2002) wing vein, with Scer\GAL4^Act5C.PI (Callus and Mathey-Prevot, 2002)
Detailed Description
	Statement Reference Expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[Mef2.247.Switch] specifically during the adult stage (in the presence of RU486) causes a striking defect in long term memory, without affecting anesthesia-resistant memory or short term memory. (Copf et al., 2011) Expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[hs.2sev] results in a lack of photoreceptor cell R7 in 7% of ommatidia. (Almudi et al., 2009) Expression of Socs36E^{Scer\UAS.P\T.cCa}, under the control of either Scer\GAL4^slbo.2.6 or Scer\GAL4^c306, inhibits border cell migration and recruitment. Such egg chambers have an average of 3.3 border cells. (Silver et al., 2005) The proliferation of germ cells in male embryos expressing Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] is reduced compared to control male embryos. (Wawersik et al., 2005) Hemocytes in Socs36E^Scer\UAS.cCa; Scer\GAL4^srp.Hemo late embryos do not exhibit abnormal aggregation. (Bruckner et al., 2004) Expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^ey.PH or Scer\GAL4^dpp.blk1 does not result in flies with enlarged eyes. (Bach et al., 2003) Expression of Socs36E^{Scer\UAS.P\T.cCa}, driven by Scer\GAL4^Act5C.PI, results in occasional wing vein defects and a significantly shorter lifespan. 94% of flies that express Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^ap-md544 show an outstretched wing phenotype. 99% of flies that express one copy of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^en-e16E exhibit a loss of the anterior cross vein. Occasional wing deltas are also observed. Almost 40% of these flies show humeral outgrowths; this phenotype has a higher penetrance when flies are raised at 29^oC. 10-20% of flies show a partial or total loss of the halteres; this phenotype is also present at higher levels at 29^oC. Finally, these flies also display a strong gradient in eye pigmentation. Flies that express two copies of the Socs36E^{Scer\UAS.P\T.cCa} transgene, under the control of two copies of Scer\GAL4^en-e16E, often die as pharate adults. Those that survive to eclose show the phenotypes described above at a higher penetrance. (Callus and Mathey-Prevot, 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has visible phenotype, enhanceable by hop²/hop[+] (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has visible phenotype, enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶ (Callus and Mathey-Prevot, 2002)
Suppressed by
Statement Reference Scer\GAL4^ap-md544, Socs36E^{Scer\UAS.P\T.cCa} has visible phenotype, suppressible \| partially by Cbl^F165/Cbl[+] (Callus and Mathey-Prevot, 2002)
Enhancer of
Statement Reference Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^ey.PH is an enhancer of visible phenotype of os^unspecified/Df(1)os1A2 (Bach et al., 2003)
Suppressor of
Statement Reference Scer\GAL4^Act.PU/Socs36E^{Scer\UAS.P\T.cCa} is a suppressor \| somatic clone of tumorigenic \| somatic clone phenotype of Sce¹ (Classen et al., 2009) Scer\GAL4^en-e16E/Socs36E^{Scer\UAS.P\T.cCa} is a suppressor \| partially of lethal phenotype of Scer\GAL4^en-e16E, hop^Scer\UAS.cHa (Callus and Mathey-Prevot, 2002) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a suppressor of visible phenotype of Scer\GAL4^hs.2sev, sev^{S11.T:Hsap\MYC} (Almudi et al., 2009)
NOT Suppressor of
Statement Reference Socs36E^{Scer\UAS.P\T.cCa}, Scer\GAL4^hs.2sev is a non-suppressor of visible phenotype of Egfr::tor^act.Scer\UAS, Scer\GAL4^hs.2sev (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a non-suppressor of visible phenotype of N^act.sev (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a non-suppressor of visible phenotype of N^nucl.sev (Almudi et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing phenotype, enhanceable by hop²/hop[+] (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing phenotype, enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶ (Callus and Mathey-Prevot, 2002)
NOT Enhanced by
Statement Reference Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has anterior crossvein phenotype, non-enhanceable by Egfr[+]/Egfr^top-EC20 (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has anterior crossvein phenotype, non-enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶ (Callus and Mathey-Prevot, 2002)
Suppressed by
Statement Reference Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has anterior crossvein phenotype, suppressible \| partially by Cbl^F165/Cbl[+] (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has anterior crossvein phenotype, suppressible by hop^Scer\UAS.cHa (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing phenotype, suppressible \| partially by Cbl^F165/Cbl[+] (Callus and Mathey-Prevot, 2002)
Enhancer of
Statement Reference Scer\GAL4^C587/Socs36E^{Scer\UAS.P\T.cCa} is an enhancer of cyst cell \| heat sensitive phenotype of Stat92E^F/Stat92E^j6C8 (Singh et al., 2010) Scer\GAL4^C587/Socs36E^{Scer\UAS.P\T.cCa} is an enhancer of cyst progenitor cell \| male \| heat sensitive phenotype of Stat92E^F/Stat92E^j6C8 (Singh et al., 2010) Scer\GAL4^C587/Socs36E^{Scer\UAS.P\T.cCa} is an enhancer of male germline stem cell \| heat sensitive phenotype of Stat92E^F/Stat92E^j6C8 (Singh et al., 2010) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^ey.PH is an enhancer of eye phenotype of os^unspecified/Df(1)os1A2 (Bach et al., 2003)
Suppressor of
Statement Reference Scer\GAL4^Act.PU/Socs36E^{Scer\UAS.P\T.cCa} is a suppressor \| somatic clone of eye disc \| somatic clone phenotype of Sce¹ (Classen et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a suppressor of eye phenotype of Scer\GAL4^hs.2sev, sev^{S11.T:Hsap\MYC} (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a suppressor of eye phenotype of sev^{S11.T:Hsap\MYC} (Almudi et al., 2010) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a suppressor of photoreceptor cell R7 \| supernumerary phenotype of Scer\GAL4^hs.2sev, sev^{S11.T:Hsap\MYC} (Almudi et al., 2009)
NOT Suppressor of
Statement Reference Socs36E^{Scer\UAS.P\T.cCa}, Scer\GAL4^hs.2sev is a non-suppressor of eye phenotype of Egfr::tor^act.Scer\UAS, Scer\GAL4^hs.2sev (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}, Scer\GAL4^hs.2sev is a non-suppressor of photoreceptor cell R7 \| supernumerary phenotype of Egfr::tor^act.Scer\UAS, Scer\GAL4^hs.2sev (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a non-suppressor of eye phenotype of N^act.sev (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a non-suppressor of eye phenotype of N^nucl.sev (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a non-suppressor of photoreceptor cell R7 \| supernumerary phenotype of lz^sev.PF (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a non-suppressor of photoreceptor cell R7 \| supernumerary phenotype of N^act.sev (Almudi et al., 2009) Socs36E^{Scer\UAS.P\T.cCa}/Scer\GAL4^hs.2sev is a non-suppressor of photoreceptor cell R7 \| supernumerary phenotype of N^nucl.sev (Almudi et al., 2009)
Other
Statement Reference Egfr[+]/Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Egfr[+]/Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein L4 phenotype (Callus and Mathey-Prevot, 2002) Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein L4 phenotype (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa}, hop²/hop[+] has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa}, hop² has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa}, Stat92E[+]/Stat92E⁰⁶³⁴⁶ has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa}, Stat92E⁰⁶³⁴⁶ has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Scer\GAL4^hs.2sev, Socs36E^{Scer\UAS.P\T.cCa}, drk^EP2477, sev^{S11.T:Hsap\MYC} has eye phenotype (Almudi et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[hs.2sev] in a sev[S11.T:Hsap\MYC] genetic background rescues the rough-eye phenotype. Expression of drk[EP2477] and Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[hs.2sev] in a sev[S11.T:Hsap\MYC] genetic background results in an intermediate rough-eye phenotype. (Almudi et al., 2010) Stat92E[F]/Stat92E[j6C8] males expressing Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[C587] show complete loss of somatic cyst progenitor cells, somatic cyst cells, germline stem cells, spermatogonia and spermatocytes after 2 days at 29[o]C. (Singh et al., 2010) Co-expression of Socs36E[Scer\UAS.P\T.cCa] suppresses the rough eye and extra R7 photoreceptor cell phenotype caused by expression of sev[S11.T:Hsap\MYC] under the control of Scer\GAL4[hs.2sev]. The rough eye and extra R7 photoreceptor cell phenotypes caused by expression of Egfr::tor[act.Scer\UAS] under the control of Scer\GAL4[hs.2sev] are not suppressed by co-expression of Socs36E[Scer\UAS.P\T.cCa]. The extra R7 photoreceptor cell phenotype caused by lz[sev.PF] is not suppressed by expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[hs.2sev]. The rough eye and extra R7 photoreceptor cell phenotypes caused by N[nucl.sev] are not suppressed by expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[hs.2sev]. The rough eye and extra R7 photoreceptor cell phenotypes caused by N[act.sev] are not suppressed by expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[hs.2sev]. (Almudi et al., 2009) Expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[Act.PU] suppresses the overgrowth of Sce[1] mutant eye discs. (Classen et al., 2009) The small eye phenotype of os^unspecified/Df(1)os1A2 flies is enhanced by expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^ey.PH. (Bach et al., 2003) Expression of Socs36E^{Scer\UAS.P\T.cCa}, under the control of Scer\GAL4^en-e16E, in a Stat92E⁰⁶³⁴⁶/+ background results in an exacerbation of the outstretched wing phenotype seen in Socs36E^{Scer\UAS.P\T.cCa} expressing flies. The outgrowth appears to be made of wing material. The missing anterior crossvein phenotype is not affected. However, 60% of these flies show ectopic venation in the wing. Likewise, expression of Socs36E^{Act5C.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E in a hop²/+ background induces ectopic vein formation in 17-26% of wings and the size and penetrance of the humeral outgrowth phenotype is increased. Coexpression of Socs36E^{Scer\UAS.P\T.cCa} and hop^Scer\UAS.cHa, under the control of Scer\GAL4^en-e16E, partially suppresses the lethality of hop^Scer\UAS.cHa-expressing flies. This coexpression results in the full restoration of the anterior crossvein, which is lost in Socs36E^{Scer\UAS.P\T.cCa}-expressing flies. Expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^en-e16E in a Egfr^top-EC20/+ background results in ectopic wing vein material in 86% of flies and occasional thinning of a section of the L4 longitudinal wing vein. The anterior crossvein loss phenotype of Socs36E^{Scer\UAS.P\T.cCa}-expressing flies is not affected. Expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^en-e16E in a Cbl^F165/+ background results in a partial rescue of the Socs36E^{Scer\UAS.P\T.cCa} anterior crossvein phenotype, with a reduction in penetrance from 95 to 48%. The penetrance of the outstretched wing phenotype of Scer\GAL4^ap-md544>Socs36E^{Scer\UAS.P\T.cCa} flies is reduced from 82 to 54% when flies have a Cbl^F165/+ background. (Callus and Mathey-Prevot, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^C587/Socs36E^{Scer\UAS.P\T.cCa} rescues Socs36E^EY06665 (Singh et al., 2010) Scer\GAL4^unspecified/Socs36E^{Scer\UAS.P\T.cCa} rescues Socs36E^PZ1647 (Issigonis et al., 2009)
Comments	Expression of Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[C587] rescues the mutant cyst progenitor cell phenotype seen in the testes of Socs36E[EY06665] males. (Singh et al., 2010) Expression of Socs36E[Scer\UAS.P\T.cCa] in a Socs36E[PZ1647] mutant background rescues the abnormal male germline stem cell number phenotype. (Issigonis et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Socs36E^Scer\UAS.cCa Socs36E^{Scer\UAS.P\T.cCa}
Name Synonym	Saccharomyces cerevisiae UAS construct a of Callus (Bach et al., 2003)
Secondary FlyBase IDs

References ( 12 )
Research paper	Copf et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(19): 8059--8064 Cytokine signaling through the JAK/STAT pathway is required for long-term memory in Drosophila. [FBrf0213703] Almudi et al., 2010, J. Cell Sci. 123(22): 3857--3862 Competition between SOCS36E and Drk modulates Sevenless receptor tyrosine kinase activity. [FBrf0212242] Singh et al., 2010, J. Cell. Physiol. 223(2): 500--510 Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling. [FBrf0210092] Almudi et al., 2009, Dev. Biol. 326(1): 212--223 SOCS36E specifically interferes with Sevenless signaling during Drosophila eye development. [FBrf0207068] Classen et al., 2009, Nat. Genet. 41(10): 1150--1155 A tumor suppressor activity of Drosophila Polycomb genes mediated by JAK-STAT signaling. [FBrf0208982] Issigonis et al., 2009, Science 326(5949): 153--156 JAK-STAT signal inhibition regulates competition in the Drosophila testis stem cell niche. [FBrf0209165] Terry et al., 2006, Dev. Biol. 294(1): 246--257 Novel regulators revealed by profiling Drosophila testis stem cells within their niche. [FBrf0190169] Silver et al., 2005, Development 132(15): 3483--3492 Requirement for JAK/STAT signaling throughout border cell migration in Drosophila. [FBrf0187483] Wawersik et al., 2005, Nature 436(7050): 563--567 Somatic control of germline sexual development is mediated by the JAK/STAT pathway. [FBrf0188143] Bach et al., 2003, Genetics 165(3): 1149--1166 A sensitized genetic screen to identify novel regulators and components of the Drosophila janus kinase/signal transducer and activator of transcription pathway. [FBrf0167623] Callus and Mathey-Prevot, 2002, Oncogene 21(31): 4812--4821 SOCS36E, a novel Drosophila SOCS protein, suppresses JAK/STAT and EGF-R signalling in the imaginal wing disc. [FBrf0151456]
Supplementary material	Bruckner et al., 2004, Dev. Cell 7(1): [FBrf0200152]

Allele Dmel\Socs36EScer\UAS.P\T.cCa

Allele Dmel\Socs36E^{Scer\UAS.P\T.cCa}