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General Information
Symbol
Dmel\Socs36EUASp.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Callus
FlyBase ID
FBal0155806
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-SOCS36E, UAS-SOCS
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASp regulatory sequences drive expression of Socs36E.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4en-e16E does not result in significant changes in the cell number of the posterior compartment of third instar larval wing discs, nor in the viability of the discs, as compared to controls.
Expression of Socs36EScer\UAS.P\T.cCa using Scer\GAL4Act.PU increases mortality rates after infection with Drosophila C virus (DCV) compared with wild-type.
Border cell cluster formation and migration is defective in females expressing Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4c306, with cells failing to reach the oocyte in 53% of stage 10 egg chambers. The phenotype ranges in severity from cases in which a small number of anterior follicle cells are clustered around the polar cells but fail to migrate, to the invasive cells being splayed out along the migratory path instead of organised into a cohesive cluster. Expression of Socs36EScer\UAS.P\T.cCa under the control of either Scer\GAL4upd or Scer\GAL4Socs16D-NP7149 does not cause border cell defects.
Expressing Socs36EUASp.cCa under the control of Scer\GAL4ap-md544 results in a significantly smaller wing disc dorsal compartment in third instar larvae compared to wild-type controls.
Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4Mef2.247.Switch specifically during the adult stage (in the presence of RU486) causes a striking defect in long term memory, without affecting anesthesia-resistant memory or short term memory.
Adult flies expressing Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4repo.PU (using tub-Gal80[ts] to minimize the transgene expression during development) do not show any increase in the number of apoptotic cells in the adult brain compared to controls.
Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev results in a lack of photoreceptor cell R7 in 7% of ommatidia.
Scer\GAL4nos.UTR.T:Hsim\VP16-mediated expression of Socs36EScer\UAS.P\T.cCa causes germline stem cell loss. Inducible Scer\GAL4Act5C.PP-mediated expression of Socs36EScer\UAS.P\T.cCa (via heat shock and hs-flp of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) does not cause a change in cell morphology or a significant loss of germline stem cells.
Expression of Socs36EScer\UAS.P\T.cCa, under the control of either Scer\GAL4slbo.2.6 or Scer\GAL4c306, inhibits border cell migration and recruitment. Such egg chambers have an average of 3.3 border cells.
The proliferation of germ cells in male embryos expressing Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is reduced compared to control male embryos.
Hemocytes in Socs36EScer\UAS.cCa; Scer\GAL4srp.Hemo late embryos do not exhibit abnormal aggregation.
Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4ey.PH or Scer\GAL4dpp.blk1 does not result in flies with enlarged eyes.
Expression of Socs36EScer\UAS.P\T.cCa, driven by Scer\GAL4Act5C.PI, results in occasional wing vein defects and a significantly shorter lifespan. 94% of flies that express Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4ap-md544 show an outstretched wing phenotype. 99% of flies that express one copy of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4en-e16E exhibit a loss of the anterior cross vein. Occasional wing deltas are also observed. Almost 40% of these flies show humeral outgrowths; this phenotype has a higher penetrance when flies are raised at 29oC. 10-20% of flies show a partial or total loss of the halteres; this phenotype is also present at higher levels at 29oC. Finally, these flies also display a strong gradient in eye pigmentation. Flies that express two copies of the Socs36EScer\UAS.P\T.cCa transgene, under the control of two copies of Scer\GAL4en-e16E, often die as pharate adults. Those that survive to eclose show the phenotypes described above at a higher penetrance.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Socs36EScer\UAS.P\T.cCa suppresses the tumor phenotypes of third instar larval wing discs observed either upon the expression of scribHMS01490 under the control of Scer\GAL4Bx-MS1096 or in dlg12/dlg1A40.2 transheterozygous (where Scer\GAL4en-e16E-driven expression leads to a significantly decreased cell number in the discs' posterior compartment).
Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4ey.PU rescues the lethality of animals carrying Scer\GAL4ey.PU mutant eye discs. The rescued adults have very reduced eyes.
Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev in a sevS11.T:Hsap\MYC genetic background rescues the rough-eye phenotype. Expression of drkEP2477 and Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev in a sevS11.T:Hsap\MYC genetic background results in an intermediate rough-eye phenotype.
Stat92EF/Stat92Ej6C8 males expressing Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4C587 show complete loss of somatic cyst progenitor cells, somatic cyst cells, germline stem cells, spermatogonia and spermatocytes after 2 days at 29[o]C.
Co-expression of Socs36EScer\UAS.P\T.cCa suppresses the rough eye and extra R7 photoreceptor cell phenotype caused by expression of sevS11.T:Hsap\MYC under the control of Scer\GAL4hs.2sev. The rough eye and extra R7 photoreceptor cell phenotypes caused by expression of Egfr::toract.Scer\UAS under the control of Scer\GAL4hs.2sev are not suppressed by co-expression of Socs36EScer\UAS.P\T.cCa. The extra R7 photoreceptor cell phenotype caused by lzsev.PF is not suppressed by expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev. The rough eye and extra R7 photoreceptor cell phenotypes caused by Nnucl.sev are not suppressed by expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev. The rough eye and extra R7 photoreceptor cell phenotypes caused by Nact.sev are not suppressed by expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev.
Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4Act.PU suppresses the overgrowth of Sce1 mutant eye discs.
bamhs.PO testes recovering after heat shock that also express Socs36EScer\UAS.P\T.cCa via Scer\GAL4Act5C.PP show a marked decrease in their ability to regain new germ line stem cells compared to recovering testes not expressing Socs36EScer\UAS.P\T.cCa.
The small eye phenotype of upd1unspecified/Df(1)os1A2 flies is enhanced by expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4ey.PH.
Expression of Socs36EScer\UAS.P\T.cCa, under the control of Scer\GAL4en-e16E, in a Stat92E06346/+ background results in an exacerbation of the outstretched wing phenotype seen in Socs36EScer\UAS.P\T.cCa expressing flies. The outgrowth appears to be made of wing material. The missing anterior crossvein phenotype is not affected. However, 60% of these flies show ectopic venation in the wing. Likewise, expression of Socs36EAct5C.T:Ivir\HA1 under the control of Scer\GAL4en-e16E in a hop2/+ background induces ectopic vein formation in 17-26% of wings and the size and penetrance of the humeral outgrowth phenotype is increased. Coexpression of Socs36EScer\UAS.P\T.cCa and hopScer\UAS.cHa, under the control of Scer\GAL4en-e16E, partially suppresses the lethality of hopScer\UAS.cHa-expressing flies. This coexpression results in the full restoration of the anterior crossvein, which is lost in Socs36EScer\UAS.P\T.cCa-expressing flies. Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4en-e16E in a Egfrtop-EC20/+ background results in ectopic wing vein material in 86% of flies and occasional thinning of a section of the L4 longitudinal wing vein. The anterior crossvein loss phenotype of Socs36EScer\UAS.P\T.cCa-expressing flies is not affected. Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4en-e16E in a CblF165/+ background results in a partial rescue of the Socs36EScer\UAS.P\T.cCa anterior crossvein phenotype, with a reduction in penetrance from 95 to 48%. The penetrance of the outstretched wing phenotype of Scer\GAL4ap-md544>Socs36EScer\UAS.P\T.cCa flies is reduced from 82 to 54% when flies have a CblF165/+ background.
Xenogenetic Interactions
Statement
Reference
The increased number of apoptotic cells in the brain of adult flies expressing Hsap\MAPTScer\UAS.cWa under the control of Scer\GAL4repo.PU (using tub-Gal80[ts] to minimize the transgene expression during development) is increased further by co-expression of Socs36EScer\UAS.P\T.cCa.
Complementation and Rescue Data
Comments
Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4C587 rescues the mutant cyst progenitor cell phenotype seen in the testes of Socs36EEY06665 males.
Expression of Socs36EScer\UAS.P\T.cCa in a Socs36EPZ1647 mutant background rescues the abnormal male germline stem cell number phenotype.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Socs36EScer\UAS.P\T.cCa
Socs36EScer\UAS.cCa
Socs36EUASp.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Callus
Secondary FlyBase IDs
    References (21)