FlyBase Allele Report: Dmel\Sdc[23]

General Information

Symbol

Dmel\Sdc²³

Species

D. melanogaster

Name

FlyBase ID

FBal0155821

Feature type

allele

Associated gene

Dmel\Sdc

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Steigemann et al., 2004)

Progenitor genotype

Sdc^EPG4752

(Steigemann et al., 2004)

Cytology

Description

Imprecise excision of the element in Sdc^EPG4752, resulting in a deletion that removes the transcription start site and the first and second exons.

(Steigemann et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

In Sdc²³ third instar larvae the 10 dorsal branches of the tracheal system fail to establish a dorsal anastomosis at the midline, a phenotype that is rarely seen in wild type or heterozygotes. The frequency of missing anastomoses varies between segments, but overall almost half of all dorsal branches fail to fuse. Removal of the maternal gene product results in an enhanced phenotype.

In Sdc²⁶³⁹/Sdc²³ third instar larvae the 10 dorsal branches of the tracheal system fail to establish a dorsal anastomosis at the midline. The frequency of missing anastomoses varies between segments (DA1-DA10), but overall approximately 40% of all dorsal branches fail to fuse. Very few defects are seen in wild type.

(Schulz et al., 2011)

Mutant embryos exhibit ventral midline crossovers of ipsilateral axon fascicles.

(Chanana et al., 2009)

Homozygous Sdc²³ mutants have a higher rate of unfused tracheal dorsal branch segments per animal compared to wild type.

(Schulz et al., 2009)

Sdc²³ mutant embryos develop variable muscle pattern defects that include the absence of lateral transverse muscles, loss of muscle fibers, and abnormally shaped muscles. The pattern of muscle attachment sites appears normal, while abnormal muscle elongations are observed in fully developed but unhatched mutant larvae.

(Staudt et al., 2005)

Homozygous embryos show a low frequency of muscles crossing the ventral midline and show inner Fas2-positive axons crossing the ventral midline. Heterozygous embryos do not show ventral muscles or inner Fas2-positive axons of the central nervous system crossing the midline.

(Steigemann et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sdc²³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by ttv^00681b

(Chanana et al., 2009)

Sdc²³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by dlp^A187

(Chanana et al., 2009)

Suppressed by

Statement

Reference

Sdc²³ has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by dlp^UAS.cBa/Scer\GAL4^elav.PU

(Chanana et al., 2009)

Sdc²³ has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by dlp^UAS.cBa/Scer\GAL4^sim.P3.7

(Chanana et al., 2009)

Sdc²³ has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by dlp^UAS.cBa/Scer\GAL4^repo.PU

(Chanana et al., 2009)

Sdc²³ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\SDC2^UAS.GFP

(Chanana et al., 2009)

Suppressor of

Statement

Reference

dlp[+], Sdc[+], dlp^A187, Sdc²³ is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Fmr1^Δ50M

(Friedman et al., 2013)

dlp[+], Sdc[+], dlp^A187, Sdc²³ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Fmr1^Δ50M

(Friedman et al., 2013)

Other

Statement

Reference

Sara^Ubi.PJ, Sdc⁴⁸/Sdc²³ has partially lethal - majority die phenotype

(Schulz et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Sdc²³ has fascicle | embryonic stage | ectopic phenotype, enhanceable by ttv^00681b

(Chanana et al., 2009)

Sdc²³ has fascicle | embryonic stage | ectopic phenotype, enhanceable by dlp^A187

(Chanana et al., 2009)

Suppressed by

Statement

Reference

Sdc²³ has fascicle | ectopic | embryonic stage phenotype, suppressible | partially by dlp^UAS.cBa/Scer\GAL4^elav.PU

(Chanana et al., 2009)

Sdc²³ has fascicle | ectopic | embryonic stage phenotype, suppressible | partially by dlp^UAS.cBa/Scer\GAL4^sim.P3.7

(Chanana et al., 2009)

Sdc²³ has fascicle | ectopic | embryonic stage phenotype, suppressible | partially by dlp^UAS.cBa/Scer\GAL4^repo.PU

(Chanana et al., 2009)

Sdc²³ has fascicle | ectopic | embryonic stage phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\SDC2^UAS.GFP

(Chanana et al., 2009)

Suppressor of

Statement

Reference

dlp[+], Sdc[+], dlp^A187, Sdc²³ is a suppressor of type I bouton | third instar larval stage phenotype of Fmr1^Δ50M

(Friedman et al., 2013)

Other

Statement

Reference

Fmr1^Δ50M, Sdc[+]/Sdc²³, dlp[+]/dlp^A187 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Friedman et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

dlp^A187/+, Sdc²³/+ mutants exhibit suppression of the increase in NMJ branching and synaptic bouton number and suppression of the increased amplitude of evoked junctional currents seen in Fmr1^Δ50M/Fmr1^Δ50M mutant larvae.

Neuromuscular junctions in dlp^A187/+, Sdc²³/+ double mutant larvae do not exhibit significant changes in branch number and increased type I synaptic bouton number, as compared to controls.

(Friedman et al., 2013)

Scer\GAL4^elav.PU-, Scer\GAL4^sim.P3.7- or Scer\GAL4^repo.PU-mediated expression of dlp^Scer\UAS.cBa partially rescues the Sdc²³ ventral midline fascicle cross-over phenotype.

ttv^00681b, Sdc²³ or dlp^A187, Sdc²³ double mutant embryos show a 2-fold increase in ventral midline crossovers of axon fascicles compared to Sdc²³ single mutants.

(Chanana et al., 2009)

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^elav.PU-mediated expression of Hsap\SDC2^{Scer\UAS.T:Avic\GFP} fully rescues the Sdc²³ ventral midline fascicle cross-over phenotype.

(Chanana et al., 2009)

Complementation and Rescue Data

Rescued by

Sdc²³ is rescued by Scer\GAL4^elav.PU/Sdc^{PA.UAS.Tag:FLAG,GFP}

(Chanana et al., 2009)

Sdc²³ is rescued by Sdc^{PA.UAS.Tag:FLAG,GFP}/Scer\GAL4^da.G32

(Chanana et al., 2009)

Sdc²³ is rescued by Sdc^{PA.UAS.Tag:FLAG,GFP}/Scer\GAL4^Mef2.PR

(Chanana et al., 2009)

Sdc²³ is rescued by Scer\GAL4^elav.PU/Sdc^{ΔC.UAS.Tag:FLAG,GFP}

(Chanana et al., 2009)

Sdc²³ is rescued by Sdc^{GPI.UAS.Tag:FLAG}/Scer\GAL4^elav.PU

(Chanana et al., 2009)

Sdc²³ is rescued by Scer\GAL4^elav.PU/Sdc^SG1.UAS.GFP

(Chanana et al., 2009)

Sdc²³ is rescued by Sdc^SG3.UAS.GFP/Scer\GAL4^elav.PU

(Chanana et al., 2009)

Sdc²³ is rescued by Scer\GAL4^elav.PU/Sdc^SG5.UAS.GFP

(Chanana et al., 2009)

Not rescued by

Sdc²³ is not rescued by Sdc^{PA.UAS.Tag:FLAG,GFP}/Scer\GAL4^sim.P3.7

(Chanana et al., 2009)

Sdc²³ is not rescued by Sdc^{PA.UAS.Tag:FLAG,GFP}/Scer\GAL4^repo.PU

(Chanana et al., 2009)

Sdc²³ is not rescued by Scer\GAL4^elav.PU/Sdc^ΔTC.UAS.GFP

(Chanana et al., 2009)

Comments

Scer\GAL4^elav.PU-, Scer\GAL4^da.G32- or Scer\GAL4^Mef2.PR-mediated expression of Sdc^{PA.Scer\UAS.T:Zzzz\FLAG,T:Avic\GFP} fully rescues the Sdc²³ ventral midline fascicle cross-over phenotype. In contrast, Scer\GAL4^sim.P3.7- or Scer\GAL4^repo.PU-mediated expression does not rescue.

Scer\GAL4^elav.PU-mediated expression of Sdc^{ΔC.Scer\UAS.T:Zzzz\FLAG,T:Avic\GFP} fully rescues the Sdc²³ ventral midline fascicle cross-over phenotype.

Scer\GAL4^elav.PU-mediated expression of Sdc^{GPI.Scer\UAS.T:Zzzz\FLAG} fully rescues the Sdc²³ ventral midline fascicle cross-over phenotype.

Scer\GAL4^elav.PU-mediated expression of Sdc^{ΔTC.Scer\UAS.T:Avic\GFP} does not rescue the Sdc²³ ventral midline fascicle cross-over phenotype.

Scer\GAL4^elav.PU-mediated expression of Sdc^{SG1.Scer\UAS.T:Avic\GFP}, Sdc^{SG3.Scer\UAS.T:Avic\GFP} or Sdc^{SG5.Scer\UAS.T:Avic\GFP} rescues the Sdc²³ ventral midline fascicle cross-over phenotype.

(Chanana et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sdc²³

(Kim et al., 2014, Koper et al., 2012)

sdc²³

(Hwang et al., 2021, Stork et al., 2014, Friedman et al., 2013, Knox et al., 2011, Schulz et al., 2011, Chanana et al., 2009, Schulz et al., 2009, Staudt et al., 2005, Steigemann et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (11)

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