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General Information
Symbol
Scer\GAL4Gr5a.8.5
Species
S. cerevisiae
Name
FlyBase ID
FBal0155850
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Gr5a-GAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An 8.5kb Gr5a promoter fragment is fused upstream of a GAL4 driver.

8kb of Gr5a upstream genomic sequence drives expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in the neurons of sugar-sensitive long, intermediate, and short labellelar taste bristles.

Scer\GAL4Gr5a.8.5 drives expression in all sweet taste receptor neurons in the labellar taste hairs, and in about 9 segmental tarsal sugar-sensing chemosensory neurons in the prothoracic leg that project to the prothoracic ganglion.

Scer\GAL4Gr5a.8.5 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Additionally, expression is driven in laterally localized multidendritic neurons with extensive arborization. Expression is also observed in projections to the abdominal ganglion.

Scer\GAL4Gr5a.8.5 is expressed in the sugar-sensitive neuron of a subset of the short labellar taste bristles.

Scer\GAL4Gr5a.8.5 is strongly expressed in the sugar-sensitive neuron of all long, intermediate and short taste sensilla of the labellum.

Scer\GAL4Gr5a.8.5 is expressed in all the sugar-sensitive receptor neurons of the gustatory sensilla of the labellum.

Scer\GAL4Gr5a.8.5 expresses in Tre (Gustatory receptor Gr5a) neurons.

Scer\GAL4Gr5a.8.5 expresses in approximately 30 neurons per labial palp, in one neuron in most sensilla.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of Scer\GAL4Gr5a.8.5 cells in adult females results in a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls.

Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Gr5a.8.5 neurons in adults does not affect wind-induced suppression of locomotion or basal walking speed, compared to controls.

Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Gr5a.8.5 cells in adults does not significantly affect preference for sucrose, compared to controls; there is a significant reduction in proboscis extension reflex probability in response to labellar, but not tarsal, sucrose, but no significant effect on short term appetitive (sucrose) or aversive olfactory memory, compared to controls; unlike controls, there is no significant sugar-induced suppression of angular or linear velocity. When inhibition is restricted to a smaller set of cells (using Scer\FLP1FLPo.oc.Tag:NLS(SV40-largeT) and Scer\GAL80αTub84B.PG) significant sugar-induced suppression of angular and linear velocity is seen, similar to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 32[o]C) of Scer\GAL4Gr5a.8.5 neurons induces a high frequency of proboscis extension, compared to controls.

Ablation of Scer\GAL4Gr5a.8.5-expressing neurons does not affect sensitivity to the gustatory sex pheromone (S,Z,Z)-CH503: as in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

Inhibition (using Ork1Δ-C.UAS.EGFP) of Scer\GAL4Gr5a.8.5 cells in adults given a choice between sucrose solution and water does not affect water or sucrose consumption, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Scer\GAL4Gr5a.8.5
Scer\GAL4Tre.8.5.cCa
Name Synonyms
Tre promoter construct of Chyb
Secondary FlyBase IDs
    References (65)