FlyBase Allele Report: Scer\GAL4[Mef2.247.Switch]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^{Mef2.247.Switch}

General Information

Symbol

Scer\GAL4^{Mef2.247.Switch}

Species

S. cerevisiae

Name

FlyBase ID

FBal0155866

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

MB-GS, MB-GS-GAL4, MBsw, MB-GeneSwitch, MB-Switch, MB247-Switch, MB-Sw, Mushroom body-Geneswitch, MB247-GeneSwitch, MBsw-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Mao et al., 2004)

Progenitor genotype

Scer\GAL4^Mef2.247

(Mao et al., 2004)

Carried in construct

Cytology

Description

The Mef2 enhancer element from Scer\GAL4^Mef2.247, fused to a minimal Hsp70Bb promoter, controls expression of the ligand-inducible GAL4::GS driver.

(Mao et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - small molecule-regulated driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

alpha/beta Kenyon cell

(Buchanan and Davis, 2010)

gamma Kenyon cell

(Buchanan and Davis, 2010)

adult mushroom body

(Awata et al., 2019, Goguel et al., 2011)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression in mushroom bodies.

(FlyBase Curators, 2017)

ScerGAL4^{Mef2.247.Switch} drives mifepristone-inducible expression in the adult mushroom body.

(Goguel et al., 2011)

Marker for

Reflects expression of

(Goguel et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | RU486 conditional, with Bhal\NaChBac^UAS.EGFP

(Joiner et al., 2006)

abnormal behavior | RU486 conditional, with Hsap\KCNJ2^UAS.EGFP

(Joiner et al., 2006)

abnormal behavior | RU486 conditional, with Mmus\Prkaca^mC.UAS

(Joiner et al., 2006)

abnormal behavior | RU486 conditional, with Sh^{EKO.UAS.GFP(GL)}

(Joiner et al., 2006)

abnormal courtship behavior | RU486 conditional, with EcR^RNAi.UAS.cIa

(Ishimoto et al., 2009)

abnormal feeding behavior | RU486 conditional, with teq^RNAi.UAS

(Colomb et al., 2009)

abnormal learning | adult stage | adult stage | RU486 conditional, with Fmr1^HMS00248

(Dong et al., 2016)

abnormal learning | adult stage | adult stage | RU486 conditional, with Nrx-1^JF02652

(Dong et al., 2016)

abnormal learning | adult stage | adult stage | RU486 conditional, with Tsc1^JF01262

(Dong et al., 2016)

abnormal learning | adult stage | adult stage | RU486 conditional, with Ube3a^JF03406

(Dong et al., 2016)

abnormal learning | adult stage | progressive | RU486 conditional, with mAcon1^RNAi.UAS

(Cho et al., 2021)

abnormal learning | adult stage | progressive | RU486 conditional, with mAcon1^UAS.cPa

(Cho et al., 2021)

abnormal learning | adult stage | RU486 conditional, with ics^RNAi.UAS.WIZ

(Ojelade et al., 2015)

abnormal learning | adult stage | RU486 conditional, with mAcon1^RNAi.UAS

(Cho et al., 2021)

abnormal learning | adult stage | RU486 conditional, with mAcon1^UAS.cPa

(Cho et al., 2021)

abnormal learning | adult stage | RU486 conditional, with Rac1^V12.UAS

(Ojelade et al., 2015)

abnormal memory, with Appl^KK102543

(Bourdet et al., 2015)

abnormal memory, with Appl^RNAi.G3.UAS

(Goguel et al., 2011)

abnormal memory, with Bper\ptxA^act.UAS

(Ferris et al., 2006)

abnormal memory, with Bper\ptxA^act.UAS, Scer\GAL80^ts.αTub84B

(Ferris et al., 2006)

abnormal memory, with Hsap\APP^{695SPA4CT.UAS.Tag:MYC}

(Goguel et al., 2011)

abnormal memory | adult stage, with sra^UAS.Tag:HA

(Shaw et al., 2015)

abnormal memory | adult stage | RU486 conditional, with Appl^{Aβ.UAS.Tag:HA}, Appl^d

(Bourdet et al., 2015)

abnormal memory | adult stage | RU486 conditional, with dbr^GD7281

(Kottler et al., 2011)

abnormal memory | adult stage | RU486 conditional, with dbr^RNAi.B.UAS

(Kottler et al., 2011)

abnormal memory | adult stage | RU486 conditional, with dbr^UAS.cKa

(Kottler et al., 2011)

abnormal memory | adult stage | RU486 conditional, with dome^RNAi.UAS.cCa

(Copf et al., 2011)

abnormal memory | adult stage | RU486 conditional, with dome^ΔCYT.UAS

(Copf et al., 2011)

abnormal memory | adult stage | RU486 conditional, with Fmr1^HMS00248

(Dong et al., 2016)

abnormal memory | adult stage | RU486 conditional, with hop^RNAi.UAS.cCa

(Copf et al., 2011)

abnormal memory | adult stage | RU486 conditional, with Hsap\APP^{695.UAS.Tag:MYC}

(Shaw et al., 2015)

abnormal memory | adult stage | RU486 conditional, with Hsap\KCNJ2^UAS.cUa.EGFP

(Liu et al., 2016)

abnormal memory | adult stage | RU486 conditional, with Inx5^KK103391

(Liu et al., 2016)

abnormal memory | adult stage | RU486 conditional, with Inx6^GD3692

(Liu et al., 2016)

abnormal memory | adult stage | RU486 conditional, with Nrx-1^JF02652

(Dong et al., 2016)

abnormal memory | adult stage | RU486 conditional, with Socs36E^UASp.cCa

(Copf et al., 2011)

abnormal memory | adult stage | RU486 conditional, with Stat92E^{M647H.UAS.EGFP}

(Copf et al., 2011)

abnormal memory | adult stage | RU486 conditional, with Stat92E^NIG.4257R

(Copf et al., 2011)

abnormal memory | adult stage | RU486 conditional, with Stat92E^ΔN.UAS

(Copf et al., 2011)

abnormal memory | adult stage | RU486 conditional, with Tsc1^JF01262

(Dong et al., 2016)

abnormal memory | adult stage | RU486 conditional, with Ube3a^JF03406

(Dong et al., 2016)

abnormal memory | adult stage | RU486 conditional, with upd1^NIG.5993R

(Copf et al., 2011)

abnormal memory | RU486 conditional, with arm^KK102545

(Tan et al., 2013)

abnormal memory | RU486 conditional, with arr^GD121

(Tan et al., 2013)

abnormal memory | RU486 conditional, with EcR^RNAi.UAS.cIa

(Ishimoto et al., 2009)

abnormal memory | RU486 conditional, with teq^RNAi.UAS

(Colomb et al., 2009)

abnormal memory | RU486 conditional, with wg^GD5007

(Tan et al., 2013)

abnormal sleep, with EcR^A.UAS

(Ishimoto and Kitamoto, 2010)

abnormal sleep, with EcR^B1.UAS

(Ishimoto and Kitamoto, 2010)

abnormal sleep, with Pka-R1^{BDK.UAS.Tag:HA}

(Crocker et al., 2010)

abnormal sleep | adult stage, with Ras85D^V12.cUa.UAS

(Machado Almeida et al., 2021)

abnormal sleep | male limited, with Hsap\DISC1^UAS.Tag:HA

(Sawamura et al., 2008)

abnormal sleep | RU486 conditional, with Mmus\Prkaca^mC.UAS.cKa

(Kuo et al., 2012)

decreased efficacy of long-term memory | adult stage, with CoRest^RNAi.UAS.C

(Takakura et al., 2021)

increased efficacy of memory | adult stage, with HDAC1^GD4513

(Takakura et al., 2021)

increased sleep | adult stage, with Nf1^KK101909

(Machado Almeida et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

Hyperpolarisation (using Hsap\KCNJ2^UAS.cUa.EGFP) of Scer\GAL4^{Mef2.247.Switch} neurons (induced by RU486 application for three days prior to the experiment) significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls.

(Liu et al., 2016)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{Mef2.247.Switch}, mAcon1^UAS.cPa has abnormal learning | adult stage | progressive | RU486 conditional phenotype, suppressible by Pink1^JF01203, Scer\GAL4^{Mef2.247.Switch}

(Cho et al., 2021)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^{Mef2.247.Switch} has abnormal memory | adult stage | RU486 conditional phenotype, suppressible | RU486 conditional by sra^UAS.Tag:HA, Scer\GAL4^{Mef2.247.Switch}

(Shaw et al., 2015)

Scer\GAL4^{Mef2.247.Switch}, sra^UAS.Tag:HA has abnormal memory | adult stage | RU486 conditional phenotype, suppressible | RU486 conditional by Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^{Mef2.247.Switch}

(Shaw et al., 2015)

Enhancer of

Statement

Reference

kuz^UAS.cFa/Scer\GAL4^{Mef2.247.Switch} is an enhancer of abnormal memory phenotype of Appl^d

(Bourdet et al., 2015)

Scer\GAL4^{Mef2.247.Switch}/Bace^UAS.cCSa is an enhancer of abnormal memory | adult stage phenotype of Appl^d

(Bourdet et al., 2015)

NOT Enhancer of

Statement

Reference

kuz^UAS.cFa/Scer\GAL4^{Mef2.247.Switch} is a non-enhancer of abnormal learning phenotype of Appl^d

(Bourdet et al., 2015)

Suppressor of

Statement

Reference

aft^UAS.Tag:FLAG/Scer\GAL4^{Mef2.247.Switch} is a suppressor of decreased efficacy of long-term memory phenotype of Cmtr1^13A, aft^M32

(Haussmann et al., 2022)

Pink1^JF01203, Scer\GAL4^{Mef2.247.Switch} is a suppressor of abnormal learning | adult stage | progressive | RU486 conditional phenotype of Scer\GAL4^{Mef2.247.Switch}, mAcon1^UAS.cPa

(Cho et al., 2021)

Adcy1^UAS.cUa/Scer\GAL4^{Mef2.247.Switch} is a suppressor of abnormal learning | adult stage phenotype of chico¹

(Naganos et al., 2016)

sra^UAS.Tag:HA, Scer\GAL4^{Mef2.247.Switch} is a suppressor | RU486 conditional of abnormal memory | adult stage | RU486 conditional phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^{Mef2.247.Switch}

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^{Mef2.247.Switch} is a suppressor | RU486 conditional of abnormal memory | adult stage | RU486 conditional phenotype of Scer\GAL4^{Mef2.247.Switch}, sra^UAS.Tag:HA

(Shaw et al., 2015)

Scer\GAL4^{Mef2.247.Switch}/DopEcR^UAS.cIb is a suppressor of abnormal memory | adult stage phenotype of Adcy1^rut-2

(Ishimoto et al., 2013)

Scer\GAL4^{Mef2.247.Switch}/DopEcR^UAS.cIb is a suppressor | RU486 conditional | drug conditional of abnormal memory | adult stage phenotype of Adcy1^rut-1084

(Ishimoto et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Mef2.247.Switch}/DopEcR^UAS.cIb is a non-suppressor | RU486 conditional | drug conditional of abnormal memory | adult stage phenotype of Adcy1^rut-1084

(Ishimoto et al., 2013)

Scer\GAL4^{Mef2.247.Switch}/5-HT1B^UAS.cYa is a non-suppressor of abnormal behavior phenotype of 5-HT1A^Δ5kb

(Yuan et al., 2006)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{Mef2.247.Switch}/Appl^UAS.cTa rescues Appl^d/Appl[+]

(Bourdet et al., 2015)

Appl^s.UAS/Scer\GAL4^{Mef2.247.Switch} rescues Appl^d

(Bourdet et al., 2015)

Scer\GAL4^{Mef2.247.Switch}/Appl^sd.UAS rescues Appl^d

(Bourdet et al., 2015)

Scer\GAL4^{Mef2.247.Switch}/arm^{S2.UAS.Tag:MYC} rescues arm^GD1372

(Tan et al., 2013)

Nf1^UAS.cBa/Scer\GAL4^{Mef2.247.Switch} rescues Nf1^c00617

(Buchanan and Davis, 2010)

Scer\GAL4^{Mef2.247.Switch}/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Mao et al., 2004)

Scer\GAL4^{Mef2.247.Switch}/Adcy1^UAS.cZa rescues Adcy1^MB2769

(Mao et al., 2004)

Fails to rescue

Appl^s.UAS/Scer\GAL4^{Mef2.247.Switch} fails to rescue Appl^d

(Bourdet et al., 2015)

Scer\GAL4^{Mef2.247.Switch}/Appl^sd.UAS fails to rescue Appl^d

(Bourdet et al., 2015)

for^UAS.cWa/Scer\GAL4^{Mef2.247.Switch} fails to rescue for^s

(Wang et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

81013

w¹¹¹⁸; P{MB-Switch}3/TM6C, Sb¹

Notes on Origin

Discoverer

Comments

Comments

The transactivation activity of the "GeneSwitch" protein expressed from this vector depends on the presence of RU486.

(Mao et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^{Mef2.247.Switch}

Name Synonyms

Secondary FlyBase IDs

References (54)

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