FlyBase Allele Report: Dmel\Pten[RNAi.Exel.UAS]

General Information

Symbol

Dmel\Pten^{RNAi.Exel.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0156019

Feature type

allele

Associated gene

Dmel\Pten

Associated Insertion(s)

Carried in Construct

P{UAS-Pten.RNAi.Exel}

Also Known As

UAS-PTEN RNAi, UAS-ptenRNAi

Key Links

Transgenic product class

RNAi_reagent

(Parks, 2004.6)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Parks, 2004.6)

Mutagen

in vitro construct

(Parks, 2004.6)

Progenitor genotype

Carried in construct

P{UAS-Pten.RNAi.Exel}

Cytology

Description

Nucleotide substitution: A423C. Nucleotides 295 through 819 from Pten sequence AF201905 were cloned in the forward and reverse directions into pJM1084 (the hairpin vector described in Reichhart et al. 2002 (FBrf0152370). The cloned sequences differed from AF201905 by an A to C change at nucleotide 423. Expression is governed by UAS regulatory sequences.

(Parks, 2004.6)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pten

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of high grade glioma

CEA with Ras85D^V12.cUa.UAS

(Gangwani et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^C57

(Ramachandran et al., 2009)

abnormal neurophysiology | larval stage, with Pten^dj189, Scer\GAL4^C57

(Ramachandran et al., 2009)

abnormal size | adult stage, with Scer\GAL4^en-e16E

(Poon et al., 2011)

visible | adult stage, with Scer\GAL4^en-e16E

(Poon et al., 2011)

Phenotype Manifest In

NMJ bouton, with Pten^dj189, Scer\GAL4^C57

(Ramachandran et al., 2009)

NMJ bouton, with Scer\GAL4^C57

(Ramachandran et al., 2009)

wing, with Scer\GAL4^en-e16E

(Poon et al., 2011)

Detailed Description

Statement

Reference

Axon regeneration of class IV da neurons in the ventral nerve cord (after axon severing at the commissure junction at 48 hours after egg laying) is enhanced in larvae expressing Pten^{dsRNA.Exel.Scer\UAS} under the control of Scer\GAL4^ppk.PG compared to wild type controls.

(Song et al., 2012)

Expression of Pten^{dsRNA.Exel.Scer\UAS} under the control of Scer\GAL4^en-e16E results in wing overgrowth.

(Poon et al., 2011)

Expression of Pten^{dsRNA.Exel.Scer\UAS} under the control of Scer\GAL4^C57 in the muscles results in a significant decrease in the number of synaptic boutons at the larval neuromuscular junction. In addition, average bouton volume is significantly increased compared to wild type.

Expression of Pten^{dsRNA.Exel.Scer\UAS} under the control of Scer\GAL4^C57 in the muscles in a Pten^dj189/+ background results in a significant decrease in the number of synaptic boutons at the larval neuromuscular junction.

Larvae expressing Pten^{dsRNA.Exel.Scer\UAS} under the control of Scer\GAL4^C57 in the muscles in a Pten^dj189/+ background show no increase in evoked excitatory junctional potential (EJP) amplitude compared to controls, although the amplitude and frequency of miniature EJPs is increased in these animals compared to wild type.

(Ramachandran et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has increased cell number | third instar larval stage phenotype, suppressible by yki^HMS00041/Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by yki^HMS00041, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has neoplasia | third instar larval stage phenotype, suppressible | partially by yki^HMS00041, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by yki^HMS00041/Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has neoplasia | third instar larval stage phenotype, suppressible | partially by yki^HMS00041/Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has increased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has neoplasia | third instar larval stage phenotype, suppressible | partially by Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has increased cell number | third instar larval stage phenotype, suppressible by Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has increased cell number | third instar larval stage phenotype, suppressible by yki^HMS00041, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

NOT suppressed by

Statement

Reference

Pten^{RNAi.Exel.UAS}, Scer\GAL4^en-e16E has abnormal size | adult stage phenotype, non-suppressible by Tao^{UASp.Tag:FLAG}, Scer\GAL4^en-e16E

(Poon et al., 2011)

Pten^{RNAi.Exel.UAS}, Scer\GAL4^en-e16E has visible | adult stage phenotype, non-suppressible by Tao^{UASp.Tag:FLAG}, Scer\GAL4^en-e16E

(Poon et al., 2011)

NOT Suppressor of

Statement

Reference

Pten^{RNAi.Exel.UAS}, Scer\GAL4^Hml.Δ is a non-suppressor of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Other

Statement

Reference

Phenotype Manifest In

Suppressed by

Statement

Reference

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has larval central brain | third instar larval stage phenotype, suppressible | partially by Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has neuroblast | increased number | third instar larval stage phenotype, suppressible by Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has neuroblast | increased number | third instar larval stage phenotype, suppressible by yki^HMS00041, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has neuroblast | increased number | third instar larval stage phenotype, suppressible by yki^HMS00041/Tep1^HMS00641, Scer\GAL4^repo.PU

(Gangwani et al., 2020)

NOT suppressed by

Statement

Reference

Pten^{RNAi.Exel.UAS}, Scer\GAL4^en-e16E has wing phenotype, non-suppressible by Tao^{UASp.Tag:FLAG}, Scer\GAL4^en-e16E

(Poon et al., 2011)

NOT Suppressor of

Statement

Reference

Pten^{RNAi.Exel.UAS}, Scer\GAL4^Hml.Δ is a non-suppressor of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Other

Statement

Reference

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has larval central brain | third instar larval stage phenotype

(Gangwani et al., 2020)

Pten^{RNAi.Exel.UAS}, Ras85D^V12.cUa.UAS, Scer\GAL4^repo.PU has neuroblast | increased number | third instar larval stage phenotype

(Gangwani et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing Pten^{dsRNA.Exel.UAS} and Ras85D^V12.cUa.UAS under the control of Scer\GAL4^repo.PU induces larval central brain gliomas; brain lobes are enlarged; there is an increase in mitosis; there is an increase in the number of neuroblasts; neuroblasts and ganglion mother cells seem to undergo a lateral shift at the edge of the central brain abutting the optic lobe.

(Gangwani et al., 2020)

Expression of Pten^{dsRNA.Exel.Scer\UAS} under the control of Scer\GAL4^sim.P3.7 or Scer\GAL4^rho.NEE has no effect on the reduced viability seen in Df(1)NetAB^Δ mutants.

Expression of Pten^{dsRNA.Exel.Scer\UAS} under the control of any of Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2}, Scer\GAL4^rho.NEE or Scer\GAL4^sim.P3.7 has no effect on the fertility or male courtship defects seen in Df(1)NetAB^Δ.

(Newquist et al., 2013)

Expression of Tao^{Scer\UAS.P\T.T:Zzzz\FLAG} is unable to suppress the wing overgrowth seen when Pten^{dsRNA.Exel.Scer\UAS} is expressed under the control of Scer\GAL4^en-e16E.

(Poon et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of Pten^{dsRNA.Exel.Scer\UAS} does not suppress the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

8550

w¹¹¹⁸; P{UAS-Pten.RNAi.Exel}3

8548

P{UAS-Pten.RNAi.Exel}1, y¹ w¹¹¹⁸