FlyBase Allele Report: Dmel\Pi3K92E[UAS.Exel]

General Information

Symbol

Dmel\Pi3K92E^UAS.Exel

Species

D. melanogaster

Name

FlyBase ID

FBal0156075

Feature type

allele

Associated gene

Dmel\Pi3K92E

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K92E.Exel}

Also Known As

UAS-PI3K, UAS-PI3K92E

Key Links

Transgenic product class

wild_type

(Parks, 2004.5.12)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Parks, 2004.5.12)

Mutagen

in vitro construct

(Parks, 2004.5.12)

Progenitor genotype

Carried in construct

P{UAS-Pi3K92E.Exel}

Cytology

Description

The coding region of wild type Pi3K92E (FBgn0015279) was cloned into P{Express-UAS}. Expression is governed by UAS regulatory sequences.

(Parks, 2004.5.12)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pi3K92E

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Eddison et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive, with Scer\GAL4^elav-C155

(Eddison et al., 2011)

increased cell death | third instar larval stage, with Scer\GAL4^en-e16E

(Sun et al., 2020)

increased cell growth rate, with Scer\GAL4^e22c

(Lv et al., 2012)

increased cell growth rate, with Scer\GAL4^GMR.PF

(Lv et al., 2012)

visible, with Scer\GAL4^Bx-MS1096

(Cliffe et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Lv et al., 2012)

ommatidium, with Scer\GAL4^GMR.PF

(Lv et al., 2012)

wing, with Scer\GAL4^Bx-MS1096

(Cliffe et al., 2009)

wing, with Scer\GAL4^e22c

(Lv et al., 2012)

wing disc | third instar larval stage, with Scer\GAL4^en-e16E

(Sun et al., 2020)

Detailed Description

Statement

Reference

Expression of Pi3K92E^UAS.Exel under the control of Scer\GAL4^en-e16E leads to an increase in cell death in third instar larval wing discs.

(Sun et al., 2020)

Overexpression of Pi3K92E^{Scer\UAS.Exel} in the developing eye, under the control of Scer\GAL4^GMR.PF, causes large, disorganised eyes.

Overexpression of Pi3K92E^{Scer\UAS.Exel} in the posterior compartment of the wing, under the control of Scer\GAL4^e22c, results in an overgrowth phenotype in the wing.

(Lv et al., 2012)

Neuronal expression of Pi3K92E^{Scer\UAS.Exel} under the control of Scer\GAL4^elav-C155 increases sensitivity to ethanol sedation.

(Eddison et al., 2011)

Expression of Pi3K92E^{Scer\UAS.Exel} under the control of Scer\GAL4^Bx-MS1096 results in a down-curled wing phenotype in 5.5% of flies.

(Cliffe et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF has increased cell growth rate phenotype, enhanceable by His4^K16A.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

Suppressed by

Statement

Reference

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF has increased cell growth rate phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

Pi3K92E^UAS.Exel, Scer\GAL4^e22c has increased cell growth rate phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^e22c

(Lv et al., 2012)

Enhancer of

Statement

Reference

Pi3K92E^UAS.Exel/Scer\GAL4^ppk.PG is an enhancer of abnormal neuroanatomy | pupal stage | somatic clone phenotype of Mical¹⁵²⁵⁶

(Wong et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Pi3K92E^UAS.Exel is a suppressor of abnormal starvation stress response | semidominant | adult stage phenotype of mir-137^CR

(Saedi et al., 2024)

Scer\GAL4^elav-C155/Pi3K92E^UAS.Exel is a suppressor of abnormal starvation stress response | semidominant | adult stage phenotype of mir-137^KO

(Saedi et al., 2024)

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, SerT^KK108310

(Pham et al., 2020)

NOT Suppressor of

Statement

Reference

Pi3K92E^UAS.Exel, Scer\GAL4^e16A is a non-suppressor of increased cell size phenotype of Scer\GAL4^e16A, trbl^UAS.Tag:FLAG

(Das et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by His4^K16A.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by His4^K16A.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

Suppressed by

Statement

Reference

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF has eye phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

Pi3K92E^UAS.Exel, Scer\GAL4^e22c has wing phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^e22c

(Lv et al., 2012)

Enhancer of

Statement

Reference

Pi3K92E^UAS.Exel/Scer\GAL4^ppk.PG is an enhancer of larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype of Mical¹⁵²⁵⁶

(Wong et al., 2013)

Suppressor of

Statement

Reference

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, SerT^KK108310

(Pham et al., 2020)

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PU is a suppressor | partially of ommatidium | adult stage phenotype of Scer\GAL4^GMR.PU, SerT^KK108310

(Pham et al., 2020)

Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PU is a suppressor | partially of interommatidial bristle | adult stage | absent phenotype of Scer\GAL4^GMR.PU, SerT^KK108310

(Pham et al., 2020)

NOT Suppressor of

Statement

Reference

Pi3K92E^UAS.Exel, Scer\GAL4^e16A is a non-suppressor of wing hair phenotype of Scer\GAL4^e16A, trbl^UAS.Tag:FLAG

(Das et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pi3K92E^{Scer\UAS.Exel} does not suppress the reduction in trichome density seen in the wings of flies expressing trbl^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^e16A.

(Das et al., 2014)

Expression of Pi3K92E^{Scer\UAS.Exel} under the control of Scer\GAL4^ppk.PG enhances the dendrite pruning defects seen in Mical¹⁵²⁵⁶ mutant ddaC neuron clones at 16 hours after puparium formation.

(Wong et al., 2013)

Depletion of Hdac3 in the eye, through expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PF, strongly suppresses the overgrown, disorganised eye phenotype (with increased ommatidial size) found upon expression of Pi3K92E^{Scer\UAS.Exel} in the eye. These double-mutant flies exhibit a phenotype similar to Hdac3^{dsRNA.Sym.Scer\UAS} single mutants.

Depletion of Hdac3 in the posterior compartment of the wing, through expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^e22c, strongly suppresses the overgrowth phenotype in the wing, upon expression of Pi3K92E^{Scer\UAS.Exel}.

Co-expression of His4^{K16A.Scer\UAS} and Pi3K92E^{Scer\UAS.Exel} in the developing eye, under the control of Scer\GAL4^GMR.PF results in an increase in eye size and the ommatidial overgrowth phenotype found upon expression of Pi3K92E^{Scer\UAS.Exel} alone.

(Lv et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

8286

y¹ w¹¹¹⁸; P{UAS-Pi3K92E.Exel}2

8287

y¹ w¹¹¹⁸; P{UAS-Pi3K92E.Exel}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pi3K92E^{Scer\UAS.Exel}

Pi3K92E^UAS.Exel

Name Synonyms

Secondary FlyBase IDs

References (15)

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