Open Close
General Information
Symbol
Dmel\MitfUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hallsson
FlyBase ID
FBal0156238
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild-type Mitf.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ectopic expression of MitfScer\UAS.cHa under the control of Scer\GAL4Lsp2.PH results in a significantly increased number of acidic vesicles and lysosomes in the fat body of both starved and fed third instar larvae compared to controls kept under the same feeding regimen. MitfScer\UAS.cHa expression also leads to accumulation of lipid droplets in both fed and starved larvae: both display the same (high) amount of lipid droplets as fed wild-type controls.

Ectopic expression of MitfScer\UAS.cHa under the control of Scer\GAL4elav.PU results in accumulation of lysosome-like structures in the brains of starved adult flies and slight accumulation of autophagosomes in the brains of fed adult flies compared to starved and fed controls, respectively.

Ectopic expression of MitfScer\UAS.cHa under the control of Scer\GAL4Lsp2.PH results in significantly increased level of lysosome-autophagosome fusion in fed third instar larvae compared to fed wild-type and (unlike in wild-type) this fusion level does not increase further upon starvation.

Expression of MitfScer\UAS.cHa under the control of Scer\GAL4c311 results in early larval lethality. Expression of MitfScer\UAS.cHa under the control of Scer\GAL4ey.PH results in eye-antennal discs with smaller eye regions than normal, but normal or nearly normal antennal regions in third instar larvae. The developing photoreceptor field in the eye disc is reduced or occasionally absent.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
MitfScer\UAS.cHa
MitfUAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hallsson
Secondary FlyBase IDs
    References (3)