Allele Dmel\Klp64D^l4

General Information
Symbol	Dmel\Klp64D^l4	Species	D. melanogaster
Name		FlyBase ID	FBal0156302
Feature type	allele	Associated gene	Dmel\Klp64D

Map ( GBrowse )
Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:5,352,053..5,352,053 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=G101D\|Klp64D-PA reported_pr_change=G101D na_change=G5352053A (Sarpal et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G101D. (Sarpal et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	auditory perception defective (with Klp64D^k5) (Sarpal et al., 2003) lethal \| recessive (Sarpal et al., 2003) neuroanatomy defective (with Klp64D^k1) (Jana et al., 2011) neuroanatomy defective (with Klp64D^k5) (Jana et al., 2011) neurophysiology defective (with Klp64D^k1) (Jana et al., 2011) neurophysiology defective (with Klp64D^k5) (Sarpal et al., 2003, Jana et al., 2011)
Phenotype Manifest In
	cilium (with Klp64D^k1) (Jana et al., 2011) cilium (with Klp64D^k5) (Jana et al., 2011) Johnston organ (with Klp64D^k5) (Sarpal et al., 2003) scolopidial cilium & Johnston's organ (with Klp64D^k5) (Sarpal et al., 2003) sensillum basiconicum of antennal segment 3 (with Klp64D^k1) (Jana et al., 2011) sensillum basiconicum of antennal segment 3 (with Klp64D^k5) (Jana et al., 2011) sensillum trichodeum of antennal segment 3 (with Klp64D^k1) (Jana et al., 2011)
Detailed Description
	Statement Reference The average peak electroantennogram response is reduced in Klp64D[k1]/Klp64D[l4] and Klp64D[k5]/Klp64D[l4] adults compared to controls (in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid). The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in Klp64D[k5]/Klp64D[l4] adults, but the outer segments of the basiconic cilia usually contain a slender extension with several singlet microtubules. The length and volume expansions of both the basiconic and trichoid cilia are reduced at the second stage of cilia growth in Klp64D[k1]/Klp64D[l4] antennae compared to wild type. (Jana et al., 2011) Klp64D^l4/Klp64D^k5 homozygotes have a reduced auditory response, producing a severely reduced sound evoked potentials from the Johnston's organ neurons. (Sarpal et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Klp64D^k5/Klp64D^l4 has auditory perception defective phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003) Klp64D^k5/Klp64D^l4 has neurophysiology defective phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003)
Other
Statement Reference Kap3^V6, Klp64D^k5/Klp64D^l4 has lethal \| partially phenotype (Sarpal et al., 2003, Sarpal et al., 2003) Kap3^V6, Klp64D^k5/Klp64D^l4 has lethal phenotype (Sarpal et al., 2003, Sarpal et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference Klp64D^k5/Klp64D^l4 has Johnston organ phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003) Klp64D^k5/Klp64D^l4 has scolopidial cilium & Johnston's organ phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The addition of Kap3^V6/+ significantly enhances the recessive lethality of Klp64D^l4/Klp64D^k5 animals. The addition of Kap3^V6/Y causes complete lethality. The addition of Kap3^V6/+ to Klp64D^l4/Klp64D^k5 also completely abolishes the sound evoked potentials usually obtained from the Johnston's organ neurons, and enhances the ciliary defects seen in these animals. (Sarpal et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Klp64D^l4 (Lee et al., 2008, Jana et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Jana et al., 2011, Mol. Biol. Cell 22(6): 769--781 Heterotrimeric kinesin-II is necessary and sufficient to promote different stepwise assembly of morphologically distinct bipartite cilia in Drosophila antenna. [FBrf0213262] Sarpal et al., 2003, Curr. Biol. 13(19): 1687--1696 Drosophila KAP interacts with the kinesin II motor subunit KLP64D to assemble chordotonal sensory cilia, but not sperm tails. [FBrf0167402]
Supplementary material	Lee et al., 2008, Curr. Biol. 18(24): Supplemental Data. [FBrf0207609]

Allele Dmel\Klp64Dl4

Allele Dmel\Klp64D^l4