|Feature type||allele||Associated gene||Dmel\Itp-r83A|
|Also Known As||itprwc703|
|Map ( GBrowse )|
|Allele class||antimorphic allele - genetic evidence|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: G2117E. Nucleotide substitution: G?A.
|Phenotype Manifest In|
The spontaneous firing rate seen in dorsal-longitudinal muscles of Itp-r83A[wc361]/Itp-r83A[wc703] adults is normal. These adults can be divided into fliers and non-fliers (assayed using a cylinder drop test). About 30% are non-fliers - exhibiting only brief rhythmic flight patterns that are usually not sustained beyond 5 seconds in response to stimulation with an air puff. In contrast, the non-fliers exhibit sustained flight patterns (>30seconds) in response to the same stimulus.
Itp-r83Awc703/Itp-r83Aug3 flies show a "down-turned" wing phenotype. Itp-r83Awc703/Itp-r83Awc361 flies show a significant degree of flightlessness but show normal wing posture. Indirect flight muscles of wild-type flies can express short bursts of electrical activity that are associated with cleaning behavior. Itp-r83Awc703/Itp-r83Aug3 flies show an increase in spontaneous firing from these muscles, which is unrelated to cleaning behavior. The indirect flight muscles of these mutants fail to produce firing patterns in response to an air puff, which occurs in wild-type muscles.
Maintenance of adaptation to a butanol source in Itp-r83Awc703 mutants is strongly defective, and recovery to 50% of preadaptation response occurs in less than one-third of the time taken in wild-type. The dominant phenotype of Itp-r83Awc703 is further accentuated by the presence of the recessive alleles Itp-r83Awc361 and Itp-r83Aug3. This suggests that the Itp-r83A dosage affects recovery from adaptation.
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361 has flightless | partially phenotype, enhanceable by Plc21Ck31911/Plc21C[+]
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361, Plc21Ck31911/Plc21C[+] has flightless | partially phenotype, suppressible by Ca-P60A[+]/Ca-P60AKum170
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361, Plc21Ck31911/Plc21C[+] has neurophysiology defective phenotype, suppressible by Ca-P60A[+]/Ca-P60AKum170
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361 has flightless | partially phenotype, suppressible | partially by GαqScer\UAS.cRa/Scer\GAL4Ddc.PL
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361 has flightless | partially phenotype, suppressible by Ca-P60A[+]/Ca-P60AKum170
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361 has neurophysiology defective phenotype, suppressible | partially by GαqScer\UAS.cRa/Scer\GAL4Ddc.PL
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361, Plc21Ck31911/Plc21C[+] has partially lethal - majority die phenotype
|Phenotype Manifest In|
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361, Plc21Ck31911/Plc21C[+] has wing phenotype, suppressible by Ca-P60A[+]/Ca-P60AKum170
Gα49B[+]/Gαq221c, Itp-r83Awc703/Itp-r83Awc361 has wing phenotype, suppressible | partially by GαqScer\UAS.cRa/Scer\GAL4Ddc.PL
The viability of Itp-r83A[wc703]/Itp-r83A[wc361] animals is unaffected by sl/sl or sl/sl. It is also unaffected by Gα49B[221c]/+, but the resulting adults have abnormal wing posture, an enhanced incidence of flight defects and mild endogenous hyperactivity of spontaneous firing recorded from the dorsal longitudinal muscles. Wing posture and flight defect phenotypes are enhanced and viability is reduced if Plc21C[k31911]/+ is also present, but partially suppressed by Gα49B[Scer\UAS.cRa]; Scer\GAL4[Ddc.PL] and completely or largely suppressed by Ca-P60A[Kum170]/+, even when Plc21C[k31911]/+ is also present.
|Complementation & Rescue Data|
|Partially rescued by|
|Not rescued by|
Complete rescue of the altered wing posture is seen in Itp-r83A[Scer\UAS.cVa]; Scer\GAL4[Ilp2.PR]/+; Itp-r83A[wc703]/Itp-r83A[ug3] animals. Flight ability is also rescued significantly in these flies. The delivery of an air puff stimulus to Itp-r83A[wc703]/Itp-r83A[ug3] flies expressing Scer\GAL4[Ilp2.PR]-driven Itp-r83A[Scer\UAS.cVa] leads to rhythmic generation of action potentials, but in most flies the air puff response lasts for a shorter period of time compared with the control flies.
Rescue of Itp-r83Awc703/Itp-r83Aug3 flies by expression of Itp-r83AScer\UAS.cVa under the control of Scer\GAL4hs.PB following a heat shock shows that expression during early pupal development is sufficient to rescue flight behavior and physiology, while expression after adult emergence is ineffective in rescuing flight.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 6 )|