Allele Dmel\Itp-r83A^wc703

General Information
Symbol	Dmel\Itp-r83A^wc703	Species	D. melanogaster
Name		FlyBase ID	FBal0156338
Feature type	allele	Associated gene	Dmel\Itp-r83A
Also Known As	itpr^wc703

Map ( GBrowse )
Allele class	antimorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	antimorphic allele - genetic evidence (Deshpande et al., 2000)
Mutagen	ethyl methanesulfonate (Joshi et al., 2004, Deshpande et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:1,361,875..1,361,875 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G1361875A pr_change=G2114E\|Itp-r83A-PA,G2123E\|Itp-r83A-PB reported_na_change=G?A reported_pr_change=G2117E (Joshi et al., 2004, Deshpande et al., 2000)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G2117E. Nucleotide substitution: G?A. (Joshi et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	flight defective (with Itp-r83A^wc361) (Banerjee et al., 2004) flightless (with Itp-r83A^ug3) (Banerjee et al., 2004) flightless \| partially (with Itp-r83A^wc361) (Banerjee et al., 2006) lethal \| cold sensitive (with Itp-r83A^ug3) (Joshi et al., 2004) lethal \| partially \| cold sensitive (with Itp-r83A^wc361) (Joshi et al., 2004) lethal \| recessive (Joshi et al., 2004) neurophysiology defective (with Itp-r83A^ug3) (Banerjee et al., 2004, Agrawal et al., 2010) viable (with Itp-r83A^wc361) (Banerjee et al., 2006) visible (with Itp-r83A^ug3) (Banerjee et al., 2004, Agrawal et al., 2010)
Phenotype Manifest In
	indirect flight muscle (with Itp-r83A^ug3) (Banerjee et al., 2004) wing (with Itp-r83A^ug3) (Banerjee et al., 2004, Agrawal et al., 2010)
Detailed Description
	Statement Reference Itp-r83A[wc703]/Itp-r83A[ug3] animals have wing posture, flight and flight physiology defects. (Agrawal et al., 2010) The spontaneous firing rate seen in dorsal-longitudinal muscles of Itp-r83A[wc361]/Itp-r83A[wc703] adults is normal. These adults can be divided into fliers and non-fliers (assayed using a cylinder drop test). About 30% are non-fliers - exhibiting only brief rhythmic flight patterns that are usually not sustained beyond 5 seconds in response to stimulation with an air puff. In contrast, the non-fliers exhibit sustained flight patterns (>30seconds) in response to the same stimulus. (Banerjee et al., 2006) Itp-r83A^wc703/Itp-r83A^ug3 flies show a "down-turned" wing phenotype. Itp-r83A^wc703/Itp-r83A^wc361 flies show a significant degree of flightlessness but show normal wing posture. Indirect flight muscles of wild-type flies can express short bursts of electrical activity that are associated with cleaning behavior. Itp-r83A^wc703/Itp-r83A^ug3 flies show an increase in spontaneous firing from these muscles, which is unrelated to cleaning behavior. The indirect flight muscles of these mutants fail to produce firing patterns in response to an air puff, which occurs in wild-type muscles. (Banerjee et al., 2004) Maintenance of adaptation to a butanol source in Itp-r83A^wc703 mutants is strongly defective, and recovery to 50% of preadaptation response occurs in less than one-third of the time taken in wild-type. The dominant phenotype of Itp-r83A^wc703 is further accentuated by the presence of the recessive alleles Itp-r83A^wc361 and Itp-r83A^ug3. This suggests that the Itp-r83A dosage affects recovery from adaptation. (Deshpande et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has flightless \| partially phenotype, enhanceable by Plc21C^k31911/Plc21C[+] (Banerjee et al., 2006) Itp-r83A^wc703/Itp-r83A^wc361 has flightless \| partially phenotype, enhanceable by Gα49B[+]/Gαq^221c (Banerjee et al., 2006)
Suppressed by
Statement Reference Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361, Plc21C^k31911/Plc21C[+] has flightless \| partially phenotype, suppressible by Ca-P60A[+]/Ca-P60A^Kum170 (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361, Plc21C^k31911/Plc21C[+] has neurophysiology defective phenotype, suppressible by Ca-P60A[+]/Ca-P60A^Kum170 (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has flightless \| partially phenotype, suppressible \| partially by Gαq^Scer\UAS.cRa/Scer\GAL4^Ddc.PL (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has flightless \| partially phenotype, suppressible by Ca-P60A[+]/Ca-P60A^Kum170 (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has neurophysiology defective phenotype, suppressible \| partially by Gαq^Scer\UAS.cRa/Scer\GAL4^Ddc.PL (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has neurophysiology defective phenotype, suppressible by Ca-P60A[+]/Ca-P60A^Kum170 (Banerjee et al., 2006)
Other
Statement Reference Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361, Plc21C^k31911/Plc21C[+] has partially lethal - majority die phenotype (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has neurophysiology defective phenotype (Banerjee et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has wing phenotype, enhanceable by Plc21C^k31911/Plc21C[+] (Banerjee et al., 2006)
Suppressed by
Statement Reference Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361, Plc21C^k31911/Plc21C[+] has wing phenotype, suppressible by Ca-P60A[+]/Ca-P60A^Kum170 (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has wing phenotype, suppressible \| partially by Gαq^Scer\UAS.cRa/Scer\GAL4^Ddc.PL (Banerjee et al., 2006) Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has wing phenotype, suppressible by Ca-P60A[+]/Ca-P60A^Kum170 (Banerjee et al., 2006)
Other
Statement Reference Gα49B[+]/Gαq^221c, Itp-r83A^wc703/Itp-r83A^wc361 has wing phenotype (Banerjee et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference The viability of Itp-r83A[wc703]/Itp-r83A[wc361] animals is unaffected by sl[1]/sl[1] or sl[2]/sl[2]. It is also unaffected by Gα49B[221c]/+, but the resulting adults have abnormal wing posture, an enhanced incidence of flight defects and mild endogenous hyperactivity of spontaneous firing recorded from the dorsal longitudinal muscles. Wing posture and flight defect phenotypes are enhanced and viability is reduced if Plc21C[k31911]/+ is also present, but partially suppressed by Gα49B[Scer\UAS.cRa]; Scer\GAL4[Ddc.PL] and completely or largely suppressed by Ca-P60A[Kum170]/+, even when Plc21C[k31911]/+ is also present. (Banerjee et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	Itp-r83A^90B.0 (Joshi et al., 2004) Itp-r83A⁰⁵⁶¹⁶ (Joshi et al., 2004) Itp-r83A^B4 (Joshi et al., 2004) Itp-r83A^ug3 (Deshpande et al., 2000) Itp-r83A^wc361 (Deshpande et al., 2000) Itp-r83A^XR12 (Joshi et al., 2004)
Rescued by	Itp-r83A^wc703/Itp-r83A^ug3 is rescued by Scer\GAL4^Ddc.PL/Itp-r83A^Scer\UAS.cVa (Banerjee et al., 2004) Itp-r83A^wc703/Itp-r83A^ug3 is rescued by Scer\GAL4^hs.PB/Itp-r83A^Scer\UAS.cVa (Banerjee et al., 2004)
Partially rescued by	Itp-r83A^wc703/Itp-r83A^ug3 is partially rescued by Scer\GAL4^Ilp2.PR/Itp-r83A^Scer\UAS.cVa (Agrawal et al., 2010)
Not rescued by	Itp-r83A^wc703/Itp-r83A^ug3 is not rescued by Scer\GAL4^Cha.7.4/Itp-r83A^Scer\UAS.cVa (Banerjee et al., 2004) Itp-r83A^wc703/Itp-r83A^ug3 is not rescued by Scer\GAL4^elav.PLu/Itp-r83A^Scer\UAS.cVa (Banerjee et al., 2004) Itp-r83A^wc703/Itp-r83A^ug3 is not rescued by Scer\GAL4^Mef2.PR/Itp-r83A^Scer\UAS.cVa (Banerjee et al., 2004) Itp-r83A^wc703/Itp-r83A^ug3 is not rescued by Scer\GAL4^Rapgap1-OK6/Itp-r83A^Scer\UAS.cVa (Banerjee et al., 2004) Itp-r83A^wc703/Itp-r83A^ug3 is not rescued by Scer\GAL4^{shakB.lethal.4.1}/Itp-r83A^Scer\UAS.cVa (Banerjee et al., 2004)
Comments	Complete rescue of the altered wing posture is seen in Itp-r83A[Scer\UAS.cVa]; Scer\GAL4[Ilp2.PR]/+; Itp-r83A[wc703]/Itp-r83A[ug3] animals. Flight ability is also rescued significantly in these flies. The delivery of an air puff stimulus to Itp-r83A[wc703]/Itp-r83A[ug3] flies expressing Scer\GAL4[Ilp2.PR]-driven Itp-r83A[Scer\UAS.cVa] leads to rhythmic generation of action potentials, but in most flies the air puff response lasts for a shorter period of time compared with the control flies. (Agrawal et al., 2010) Rescue of Itp-r83A^wc703/Itp-r83A^ug3 flies by expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^hs.PB following a heat shock shows that expression during early pupal development is sufficient to rescue flight behavior and physiology, while expression after adult emergence is ineffective in rescuing flight. (Banerjee et al., 2004)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Allelic series: Itp-r83A^sv35 >= Itp-r83A^ka901 > Itp-r83A^sa54 >= Itp-r83A^ka1091 > Itp-r83A^wc703 > Itp-r83A^ug3 > Itp-r83A^wc361. (Joshi et al., 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Itp-r83A^wc703 itpr^wc703 (Srikanth et al., 2004, Banerjee et al., 2004, Banerjee et al., 2006, Agrawal et al., 2010) wc703 (Joshi et al., 2004, )
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Agrawal et al., 2010, J. Neurosci. 30(4): 1301--1313 Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight. [FBrf0209847] Banerjee et al., 2006, J. Neurosci. 26(32): 8278--8288 Compensation of inositol 1,4,5-trisphosphate receptor function by altering sarco-endoplasmic reticulum calcium ATPase activity in the Drosophila flight circuit. [FBrf0194925] Banerjee et al., 2004, J. Neurosci. 24(36): 7869--7878 Loss of flight and associated neuronal rhythmicity in inositol 1,4,5-trisphosphate receptor mutants of Drosophila. [FBrf0180425] Joshi et al., 2004, Genetics 166(1): 225--236 Genetic dissection of itpr gene function reveals a vital requirement in aminergic cells of Drosophila larvae. [FBrf0174703] Srikanth et al., 2004, Biophys. J. 86(6): 3634--3646 Functional properties of the Drosophila melanogaster inositol 1,4,5-trisphosphate receptor mutants. [FBrf0179452] Deshpande et al., 2000, J. Neurobiol. 43(3): 282--288 The inositol 1,4,5-trisphosphate receptor is required for maintenance of olfactory adaptation in Drosophila antennae. [FBrf0128448]

Allele Dmel\Itp-r83Awc703

Allele Dmel\Itp-r83A^wc703