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Allele Dmel\Itp-r83A^ka1091

General Information
Symbol	Dmel\Itp-r83Aka1091	Species	D. melanogaster
Name		FlyBase ID	FBal0156349
Feature type	allele	Associated gene	Dmel\Itp-r83A
Also Known As	itprka1091
Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Joshi et al., 2004)
Mutations Mapped to the Genome
Type Location Additional Notes References point mutation 3R:1,361,196..1,361,196 evidence=experimental na_change=G1361196A pr_change=G1888S|Itp-r83A-PA,G1897S|Itp-r83A-PB reported_na_change=G?A reported_pr_change=G1891S (Joshi et al., 2004)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G1891S. Nucleotide substitution: G?A. (Joshi et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective | cold sensitive (with Itp-r83Aug3) (Joshi et al., 2004) fertile | poor (with Itp-r83Aug3) (Joshi et al., 2004) flight defective (with Itp-r83Aug3) (Agrawal et al., 2010) flight defective (with Itp-r83Awc361) (Banerjee et al., 2004) flightless (with Itp-r83Aug3) (Joshi et al., 2004, Banerjee et al., 2004) flightless | partially (with Itp-r83Aug3) (Banerjee et al., 2006) lethal | cold sensitive (with Itp-r83Aug3) (Banerjee et al., 2006) lethal | larval stage | cold sensitive (with Itp-r83Aug3) (Joshi et al., 2004) lethal | recessive (Joshi et al., 2004) neurophysiology defective (with Itp-r83Aug3) (Banerjee et al., 2004, Banerjee et al., 2006, Agrawal et al., 2010) visible (with Itp-r83Aug3) (Banerjee et al., 2004, Agrawal et al., 2010)
Phenotype Manifest In
	dorsal medial muscle (with Itp-r83Aug3) (Banerjee et al., 2006) indirect flight muscle (with Itp-r83Aug3) (Banerjee et al., 2004) wing (with Itp-r83Aug3) (Banerjee et al., 2004, Banerjee et al., 2006, Agrawal et al., 2010)
Detailed Description
	Statement Reference Itp-r83A[ka1091]/Itp-r83A[ug3] animals have wing posture, flight and flight physiology defects. In primary neuronal cultures obtained from brains of Itp-r83A[ka1091]/Itp-r83A[ug3] mutant larvae, store operated Ca2[2+] entry is reduced compared to controls. (Agrawal et al., 2010) Itp-r83A[ka1091]/Itp-r83A[ug3] mutants exhibit an altered wing posture. Itp-r83A[ka1091]/Itp-r83A[ug3] mutants are cold-sensitive and lethal during the third-instar larval stage at 17.5[o]C. SOCE and '[Ca[2+]'][[ER]] in these conditions are similar to wild-type neurons grown under identical conditions at 17.5[o]C. Primary neurons from Itp-r83A[ka1091]/Itp-r83A[ug3] mutant larval brains exhibit a greatly diminished SOC influx, whereas '[Ca[2+]'][[ER]] is significantly elevated in neurons derived from Itp-r83A[ka1091]/Itp-r83A[ug3] larvae grown at 25[o]c, compared to wild-type. The mean '[Ca[2+]'][[ER]] in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants appears twice as eleavated as n wild-type. The percentage of cells with detectable soc is ~3-5%, as compared with 70-80% in wild-type. Ca[2+] release through the InsP[[3]]R, due to the agonist carbachol is attenuated in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants. (Venkiteswaran and Hasan, 2009) Microsomes prepared from either adult or larval heads of Itp-r83A[ka1091]/Itp-r83A[ug3] animals show lower levels of Ca[2+] release after after InsP3 stimulation compared to microsomal vesicles from wild-type animals of a comparable developmental stage. Itp-r83A[ka1091]/Itp-r83A[ug3] is lethal at 17'C but not at 25'C. The resulting adults have an abnormal wing posture. The dorsal longitudinal flight muscles of these adults display a considerably higher frequency of spontaneous action potentials than wild-type. Unlike wild-type, the wings of these flies do not initiate any rhythmic flight patterns in response to stimulation with a gentle puff of air. (Banerjee et al., 2006) Itp-r83Aka1091/Itp-r83Aug3 flies show a "down-turned" wing phenotype. Itp-r83Aka1091/Itp-r83Awc361 flies show normal wing posture but do show a significant degree of flightlessness. Indirect flight muscles of wild-type flies can express short bursts of electrical activity that are associated with cleaning behavior. Itp-r83Aka1091/Itp-r83Aug3 flies show an increase in spontaneous firing from these muscles, which is unrelated to cleaning behavior. The indirect flight muscles of these mutants fail to produce firing patterns in response to an air puff, which occurs in wild-type muscles. The giant fiber pathway appears to have the same properties in Itp-r83Aka1091/Itp-r83Aug3 mutants as in wild type. (Banerjee et al., 2004) When Itp-r83Aug3/Itp-r83Aka1091 animals are grown at 15oC, development appears normal with low levels of pupal lethality. At 17.5oC almost all larvae die during the second instar, those few that do molt into third instar do so later than controls. Occasionally a few late pupae are also seen. Larvae exhibit lethality when shifted from 25oC to 17.5oC as second instars at 48-56 hr after egg laying (AEL), but not when shifted as third instars at 80-88 hrs AEL. Shifting the larvae back into 25oC as late second instars at 164-172 AEL does not improve viability. (Joshi et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Itp-r83Aug3/Itp-r83Aka1091 has flight defective phenotype, suppressible | partially by Scer\GAL4elav-C155/StimScer\UAS.cAa/olf186-FScer\UAS.cZa (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has flight defective phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ddc.PL (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has flight defective phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has flight defective phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR/olf186-FScer\UAS.cZa (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has flightless | partially phenotype, suppressible | partially by Ca-P60A[+]/Ca-P60AKum170 (Banerjee et al., 2006) Itp-r83Aug3/Itp-r83Aka1091 has lethal | cold sensitive phenotype, suppressible by Ca-P60A[+]/Ca-P60AKum170 (Banerjee et al., 2006) Itp-r83Aug3/Itp-r83Aka1091 has neurophysiology defective phenotype, suppressible | partially by Ca-P60A[+]/Ca-P60AKum170 (Banerjee et al., 2006) Itp-r83Aug3/Itp-r83Aka1091 has neurophysiology defective phenotype, suppressible | partially by Scer\GAL4elav-C155/StimScer\UAS.cAa/olf186-FScer\UAS.cZa (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has neurophysiology defective phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ddc.PL (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has neurophysiology defective phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has neurophysiology defective phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR/olf186-FScer\UAS.cZa (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has visible phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ddc.PL (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has visible phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has visible phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR/olf186-FScer\UAS.cZa (Agrawal et al., 2010)
Phenotype Manifest In
Suppressed by
Statement Reference Itp-r83Aug3/Itp-r83Aka1091 has dorsal medial muscle phenotype, suppressible | partially by Ca-P60A[+]/Ca-P60AKum170 (Banerjee et al., 2006) Itp-r83Aug3/Itp-r83Aka1091 has wing phenotype, suppressible | cold sensitive by Ca-P60A[+]/Ca-P60AKum170 (Banerjee et al., 2006) Itp-r83Aug3/Itp-r83Aka1091 has wing phenotype, suppressible | partially by Scer\GAL4elav-C155/StimScer\UAS.cAa/olf186-FScer\UAS.cZa (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has wing phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ddc.PL (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has wing phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 has wing phenotype, suppressible by StimScer\UAS.cAa/Scer\GAL4Ilp2.PR/olf186-FScer\UAS.cZa (Agrawal et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Stim[Scer\UAS.cAa] and olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[Ilp2.PR] completely suppresses the wing posture, flight and flight physiology defects of Itp-r83A[ka1091]/Itp-r83A[ug3] flies. Expression of Stim[Scer\UAS.cAa] driven by either Scer\GAL4[Ilp2.PR] or Scer\GAL4[Ddc.PL] suppresses the altered wing posture of Itp-r83A[ka1091]/Itp-r83A[ug3] flies as well as the spontaneous hyperactivity of the dorsal longitudinal muscles. The flight ability and sustained air-puff-induced rhythmic flight patterns are also restored in these flies. Pan-neuronal expression of Stim[Scer\UAS.cAa] and olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[elav-C155] in Itp-r83A[ka1091]/Itp-r83A[ug3] flies suppresses the abnormal wing-posture to varying extents but does not fully restore flight ability. Overexpressing Stim[Scer\UAS.cAa] with or without olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[Ilp2.PR] in Itp-r83A[ka1091]/Itp-r83A[ug3] mutant neurons restores global intracellular Ca[2+] homeostasis. (Agrawal et al., 2010) Expression of olf186-F[Scer\UAS.cZa] in Ilp2 neurons (under the control of Scer\GAL4[Ilp2.PR]) partially suppresses the altered wing posture of Itp-r83A[ka1091]/Itp-r83A[ug3] mutants. Although flight ability is not restored, flight patterns are initiated on air-puff delivery, while these are normally completely lacking in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants. Spontaneous hyperactivity of the DLMs in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants is suppressed to a significant extent by expression of olf186-F[Scer\UAS.cZa] either ubiquitously (Scer\GAL4[hs.PB] at 25[o]C) or in the aminergic (Scer\GAL4[Ilp2.PR]) and glutamatergic subneuronal domains (Scer\GAL4[OK371]). Ubiquitous expression of olf186-F[Scer\UAS.cZa] under the control of Scer\GAL4[hs.PB] in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants, up-regulated by a heat shock either in 24hr pupae or in 1-day-old adults results in a significant number of flies initiating flight in response to an air puff. Thus, levels of olf186-F[Scer\UAS.cZa] can modulate flight circuit activity both during its development and in adult function. However, the flight patterns obtained are not sustained and appear arrhythmic, indicating that although olf186-F[Scer\UAS.cZa] overexpression can suppress the flight defects and associated physiology of Itp-r83A[ka1091]/Itp-r83A[ug3] mutants to a significant extent, it is insufficient to regain complete flight. Pan-neural overexpression of olf186-F[Scer\UAS.cZa] in Itp-r83A[ka1091]/Itp-r83A[ug3] neurons restores detectable SOCE to 70%, as in wild-type. Also, '[Ca[2+]'][[ER]] is restored to wild-type levels, indicating that reduced SOCE and elevated '[Ca[2+]'][[ER]] in Itp-r83A[ka1091]/Itp-r83A[ug3] are linked homeostatic processes. Overexpression of olf186-F[Scer\UAS.cZa] in wild-type neurons (under the control of Scer\GAL4[elav-C155]) does not afffect SOCE and '[Ca[2+]'][[ER]], although it does elevate '[Ca[2+]'][[i]] to ~1υM in wild-type and Itp-r83A[ka1091]/Itp-r83A[ug3] backgrounds. Introduction of a single copy of olf186-F[k11505] suppresses the cold-sensitive lethality found in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants at 17.5[o]C. The wing posture defect found in these mutants when grown at 25[o]C is also suppressed to a significant extent. Ca-P60A[Kum170]/olf186-F[EY01467]; Itp-r83A[ka1091]/Itp-r83A[ug3] flies exhibit normal wings and normal levels of spontaneous electrical activity in DLM recordings. Flight ability is restored in a significant number of these triple mutant flies. This is in contrast to the complete loss of flight ability in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants and in olf186-F[EY01467]/+; Itp-r83A[ka1091]/Itp-r83A[ug3] or Ca-P60A[Kum170]/+; Itp-r83A[ka1091]/Itp-r83A[ug3] mutants. Over 60% of olf186-F[k11505]/Ca-P60A[Kum170]; Itp-r83A[ka1091]/Itp-r83A[ug3] adults and approximately 50% of olf186-F[EY01467]/Ca-P60A[Kum170]; Itp-r83A[ka1091]/Itp-r83A[ug3] adults pass as 'fliers' in the cylinder drop test assay. Air-puff delivery elicited sustainable rhythmic flight patterns similar to wild-type in a high proportion of these flies. The presence of Ca-P60A[Kum170] has no effect on Ca[2+] release through the InsP[[3]]R on carbachol stimulation in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants. The presence of olf186-F[EY01467] in either olf186-F[EY01467]/+; Itp-r83A[ka1091]/Itp-r83A[ug3] or olf186-F[EY01467]/Ca-P60A[Kum170]; Itp-r83A[ka1091]/Itp-r83A[ug3] organisms restored carbachol-stimulated Ca[2+] release to wild-type levels. (Venkiteswaran and Hasan, 2009) The abnormal wing posture phenotype of Itp-r83A[ka1091]/Itp-r83A[ug3] animals raised at 25'C is suppressed by Ca-P60A[Kum170]/+. Lethality due to Itp-r83A[ka1091]/Itp-r83A[ug3] in animals raised at 17'C is also suppressed by Ca-P60A[Kum170]/+. The resulting animals have the same abnormal wing posture phenotype as Itp-r83A[ka1091]/Itp-r83A[ug3] animals raised at 25'C. The flight defects seen in Itp-r83A[ka1091]/Itp-r83A[ug3] animals raised at 25'C as measured by the "cylinder drop assay" test are not suppressed by Ca-P60A[Kum170]/+. However, the abnormally high frequency of spontaneous action potentials in dorsal longitudinal flight muscles of these adults and their lack of flight response to stimulation with a gentle puff of air are both significantly suppressed by Ca-P60A[Kum170]/+. (Banerjee et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	Itp-r83A90B.0 (Joshi et al., 2004) Itp-r83A05616 (Joshi et al., 2004) Itp-r83AB4 (Joshi et al., 2004) Itp-r83AXR12 (Joshi et al., 2004)
Rescued by	Itp-r83Aug3/Itp-r83Aka1091 is rescued by Scer\GAL4Ddc.PL/Itp-r83AScer\UAS.cVa (Banerjee et al., 2004) Itp-r83Aug3/Itp-r83Aka1091 is rescued by Scer\GAL4elav-C155/Itp-r83AScer\UAS.cVa (Banerjee et al., 2006) Itp-r83Aug3/Itp-r83Aka1091 is rescued by Scer\GAL4hs.PB/Itp-r83AScer\UAS.cVa (Banerjee et al., 2004) Itp-r83Aug3/Itp-r83Aka1091 is rescued by Scer\GAL4Ilp2.PR/Scer\GAL80ts.αTub84B/Itp-r83AScer\UAS.cVa (Agrawal et al., 2010)
Partially rescued by	Itp-r83Aug3/Itp-r83Aka1091 is partially rescued by Scer\GAL4elav-C155/Itp-r83AScer\UAS.cVa (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 is partially rescued by Scer\GAL4Ilp2.PR/Itp-r83AScer\UAS.cVa (Agrawal et al., 2010) Itp-r83Aug3/Itp-r83Aka1091 is partially rescued by Scer\GAL4neur-GAL4-A101/Itp-r83AScer\UAS.cVa (Joshi et al., 2004, Banerjee et al., 2004)
Comments	Complete rescue of the altered wing posture is seen in Itp-r83A[Scer\UAS.cVa]; Scer\GAL4[Ilp2.PR]/+; Itp-r83A[ka1091]/Itp-r83A[ug3] animals. Flight ability is also rescued significantly in these flies. The delivery of an air puff stimulus to Itp-r83A[ka1091]/Itp-r83A[ug3] flies expressing Scer\GAL4[Ilp2.PR]-driven Itp-r83A[Scer\UAS.cVa] leads to rhythmic generation of action potentials similar to what is observed in wild-type flies. However, the response in some flies is more erratic and could not be maintained for more than five seconds. Wing posture and air puff-induced flight (assessed in single fly tethered assays) are not rescued when Itp-r83A[Scer\UAS.cVa]; Scer\GAL4[Ilp2.PR]/Scer\GAL80[ts.αTub84B]; Itp-r83A[ka1091]/Itp-r83A[ug3] animals are grown at 19[o]C during pupal and adult stages, but are completely rescued when these animals are grown at 30[o]C. Itp-r83A[Scer\UAS.cVa]; Scer\GAL4[Ilp2.PR]/Scer\GAL80[ts.αTub84B]; Itp-r83A[ka1091]/Itp-r83A[ug3] organisms grown at 30[o]C during pupal stages, but shifted to 19[o]C as adults, have normal wing posture and air puff-induced flight. (Agrawal et al., 2010)
Stocks ( 1 )
Bloomington	30739 w*; Itp-r83Aka1091/TM6B, Tb1
Notes on Origin
Discoverer
Comments
	Allelic series: Itp-r83Asv35 >= Itp-r83Aka901 > Itp-r83Asa54 >= Itp-r83Aka1091 > Itp-r83Awc703 > Itp-r83Aug3 > Itp-r83Awc361. (Joshi et al., 2004)
External Crossreferences & Linkouts
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Itp-r83Aka1091   itprka1091 (Banerjee et al., 2004, Banerjee et al., 2006, Venkiteswaran and Hasan, 2009, Agrawal et al., 2010) ka1091 (Joshi et al., 2004)
Name Synonym
Secondary FlyBase IDs
References ( 5 )
Research paper	Agrawal et al., 2010, J. Neurosci. 30(4): 1301--1313 Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight. [FBrf0209847] Venkiteswaran and Hasan, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(25): 10326--10331 Intracellular Ca2+ signaling and store-operated Ca2+ entry are required in Drosophila neurons for flight. [FBrf0208231] Banerjee et al., 2006, J. Neurosci. 26(32): 8278--8288 Compensation of inositol 1,4,5-trisphosphate receptor function by altering sarco-endoplasmic reticulum calcium ATPase activity in the Drosophila flight circuit. [FBrf0194925] Banerjee et al., 2004, J. Neurosci. 24(36): 7869--7878 Loss of flight and associated neuronal rhythmicity in inositol 1,4,5-trisphosphate receptor mutants of Drosophila. [FBrf0180425] Joshi et al., 2004, Genetics 166(1): 225--236 Genetic dissection of itpr gene function reveals a vital requirement in aminergic cells of Drosophila larvae. [FBrf0174703]