Allele Dmel\GNBP1^e03371

General Information
Symbol	Dmel\GNBP1^e03371	Species	D. melanogaster
Name		FlyBase ID	FBal0156443
Feature type	allele	Associated gene	Dmel\GNBP1
Also Known As	GNBP1^osi

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Gobert et al., 2003)
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:18,669,423..18,669,423 (Gobert et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion of PBac{RB} into the second exon of GNBP1 at nucleotide 511, corresponding to amino acid 48 of the preprotein or residue 29 of the predicted mature protein. (Gobert et al., 2003)
Caused by insertion	PBac{RB}GNBP1^e03371 (Gobert et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective (Gobert et al., 2003, Bischoff et al., 2004, Wang et al., 2008)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant flies are susceptible to S. aureus infection compared to wild-type adults. (Wang et al., 2008) Hemolymph clots from GNBP1[e03371] third instar larvae show normal melanization. (Bidla et al., 2007) Mutant flies show reduced survival compared to wild-type controls after infection with S. aureus. (Bischoff et al., 2004) Survival rates of mutant flies after injection of Enterococcus faecalis and Streptococcus pyogenes are severely compromised and similar to those of Dif mutants. (Gobert et al., 2003)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhanced by
Statement Reference GNBP1^e03371 has immune response defective phenotype, non-enhanceable by PGRP-SA^seml (Gobert et al., 2003)
NOT Enhancer of
Statement Reference GNBP1^e03371 is a non-enhancer of immune response defective phenotype of PGRP-SA^seml (Gobert et al., 2003)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference PGRP-SD[Δ3] GNBP1[e03371] and GNBP1[e03371] PGRP-SA[seml] double mutant flies are extremely susceptible to S. aureus infection compared to wild-type adults. (Wang et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	GNBP1^e03371 is rescued by Scer\GAL4^hs.PB/GNBP1^Scer\UAS.cGa (Gobert et al., 2003)
Comments
Stocks ( 2 )
Bloomington	18150 w¹¹¹⁸; PBac{RB}GNBP1^e03371
Harvard	- PBac{RB}GNBP1^e03371
Notes on Origin
Discoverer

Comments
	Mutants show a significant decrease in the expression of Drs in response to several Gram-positive bacterial strains, but not generally in response to fungi. Dpt expression is normal after a challenge with Gram-negative bacteria. Transfer of hemolymph from wild type to GNBP1^e03371 flies restores the inducibility of Drs by Gram-positive challenge. Excision of the PBac{RB} element is accompanied by reversion of the E. faecalis sensitivity mutant phenotype. (Gobert et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	GNBP1^e03371 GNBP1^osi (Gobert et al., 2003, Gottar et al., 2006, Wang et al., 2006, Bischoff et al., 2004, Bidla et al., 2007, Wang et al., 2008) GNBP1^Osiris (El Chamy et al., 2008)
Name Synonym	Osiris (El Chamy et al., 2008)
Secondary FlyBase IDs

References ( 8 )
Research paper	El Chamy et al., 2008, Nat. Immunol. 9(10): 1165--1170 Sensing of 'danger signals' and pathogen-associated molecular patterns defines binary signaling pathways 'upstream' of Toll. [FBrf0207060] Wang et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(33): 11881--11886 Peptidoglycan recognition protein-SD provides versatility of receptor formation in Drosophila immunity. [FBrf0205873] Bidla et al., 2007, J. Cell Sci. 120(7): 1209--1215 Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger. [FBrf0192653] Gottar et al., 2006, Cell 127(7): 1425--1437 Dual detection of fungal infections in Drosophila via recognition of glucans and sensing of virulence factors. [FBrf0194922] Wang et al., 2006, EMBO J. 25(20): 5005--5014 Sensing of Gram-positive bacteria in Drosophila: GNBP1 is needed to process and present peptidoglycan to PGRP-SA. [FBrf0194406] Bischoff et al., 2004, Nat. Immunol. 5(11): 1175--1180 Function of the Drosophila pattern-recognition receptor PGRP-SD in the detection of Gram-positive bacteria. [FBrf0180563] Gobert et al., 2003, Science 302(5653): 2126--2130 Dual activation of the Drosophila toll pathway by two pattern recognition receptors. [FBrf0168033]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]

Allele Dmel\GNBP1e03371

Allele Dmel\GNBP1^e03371