Allele Dmel\Dcr-2^R416X

General Information
Symbol	Dmel\Dcr-2^R416X	Species	D. melanogaster
Name		FlyBase ID	FBal0156732
Feature type	allele	Associated gene	Dmel\Dcr-2

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Lee et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:13,467,010..13,467,010 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=R416@\|Dcr-2-PA reported_pr_change=R416@ na_change=C13467010T (Lee et al., 2004)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: R416@. (Lee et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective \| conditional (Mueller et al., 2010) suppressor of variegation (with Dcr-2^L811fsX) (Fagegaltier et al., 2009) suppressor of variegation \| dominant (Fagegaltier et al., 2009) suppressor of variegation \| partially, with P{T1(-FRT)}T190-177 (Haynes et al., 2006) suppressor of variegation \| partially, with P{T1}T190-177 (Haynes et al., 2006) wild-type (Lee et al., 2004)
Phenotype Manifest In

Detailed Description
	Statement Reference Dcr-2[R416X] mutant flies show severely compromised survival when infected with vesicular stomatitis virus, and die within 12 days of infection. Viral titres are significantly higher compared to controls. (Mueller et al., 2010) Dcr-2[R416X]/+ significantly suppresses the position effect variegation of the Sb[1] allele that is seen in the T(2;3)Sb[V] chromosome. Dcr-2[L811fsX]/Dcr-2[R416X] suppresses the position effect variegation of the w gene that is seen in the In(1)w[m4] chromosome. (Fagegaltier et al., 2009) flies show a slight disruption of the heterochromatic silencing of the P{T1} reporter in line P{T1}T190-177 and of the P{T1(-FRT)} reporter in line P{T1(-FRT)}T190-177. (Haynes et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Dcr-1^Q1147X/Dcr-1[+], Dcr-2^R416X has female fertile phenotype (Kalidas et al., 2008)
Phenotype Manifest In
Suppressor of
Statement Reference Dcr-2^R416X is a suppressor of eye phenotype of w^IR.GMR (Lee et al., 2004) Dcr-2^R416X is a suppressor of pigment cell phenotype of w^IR.GMR (Lee et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Dcr-2[R416X] ; Dcr-1[Q1147X]/+ females have normal ovaries and normal fertility. (Kalidas et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Dcr-2^R416X (Deddouche et al., 2008, Mueller et al., 2010) dcr-2^R416X (Haynes et al., 2006, Kalidas et al., 2008) dcr2^R416X (Fagegaltier et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Mueller et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(45): 19390--19395 RNAi-mediated immunity provides strong protection against the negative-strand RNA vesicular stomatitis virus in Drosophila. [FBrf0212288] Fagegaltier et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(50): 21258--21263 The endogenous siRNA pathway is involved in heterochromatin formation in Drosophila. [FBrf0209635] Liu et al., 2009, Science 325(5941): 750--753 C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation. [FBrf0208622] Deddouche et al., 2008, Nat. Immunol. 9(12): 1425--1432 The DExD/H-box helicase Dicer-2 mediates the induction of antiviral activity in drosophila. [FBrf0206193] Kalidas et al., 2008, Mech. Dev. 125(5-6): 475--485 Drosophila R2D2 mediates follicle formation in somatic tissues through interactions with Dicer-1. [FBrf0205524] Haynes et al., 2006, Curr. Biol. 16(22): 2222--2227 Element 1360 and RNAi components contribute to HP1-dependent silencing of a pericentric reporter. [FBrf0193115] Lee et al., 2004, Cell 117(1): 69--81 Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. [FBrf0174449]

Allele Dmel\Dcr-2R416X

Allele Dmel\Dcr-2^R416X