Allele Dmel\Dcr-2^L811fsX

General Information
Symbol	Dmel\Dcr-2^L811fsX	Species	D. melanogaster
Name		FlyBase ID	FBal0156733
Feature type	allele	Associated gene	Dmel\Dcr-2
Also Known As	dcr2^L811fsX, Dcr-2^L811X, dicer-2^L811fsX

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Grimaud et al., 2006)
Mutagen	ethyl methanesulfonate (Lee et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References sequence variant 2R:13,465,636..13,465,638 comment=Leu 811 is replaced by FGIRSLCWIVAARRTPTWX. evidence=experimental (Lee et al., 2004)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The Leucine at residue 811 is replaced by FGIRSLCWIVAARRTPTWX. (Lee et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective (Wang et al., 2006, van Rij et al., 2006, Galiana-Arnoux et al., 2006) lethal \| recessive (Vagin et al., 2006) suppressor of variegation (with Dcr-2^R416X) (Fagegaltier et al., 2009) suppressor of variegation \| dominant (Fagegaltier et al., 2009) wild-type (Lee et al., 2004)
Phenotype Manifest In

Detailed Description
	Statement Reference More than 60% of cells in anaphase mutant wing discs contain lagging chromosomes. (Pek and Kai, 2011) Dcr-2[L811fsX]/+ significantly suppresses the position effect variegation of the Sb[1] allele that is seen in the T(2;3)Sb[V] chromosome. Dcr-2[L811fsX]/Dcr-2[R416X] suppresses the position effect variegation of the w gene that is seen in the In(1)w[m4] chromosome. (Fagegaltier et al., 2009) Homozygous projection neurons show normal dendrite and axon targeting. (Berdnik et al., 2008) Dcr-2[L811fsX] mutant germline stem cells (GSCs) do not exhibit any maintenance defects when clones are induced either during larval/pupal stages or during adult stages. (Shcherbata et al., 2007) Mutant flies are more susceptible to infection with RNA viruses (Flock House virus, Drosophila C virus or Sindbis virus) than control flies. (Galiana-Arnoux et al., 2006) Embryos derived from Dcr-2^L811fsX mothers exhibit normal cellularization and net lipid-droplet transport. (Meyer et al., 2006) Dcr-2^L811fsX homozygous flies fail to produce siRNA from a transfected construct. (Vagin et al., 2006) Mutant flies are hypersensitive to infection with Cricket Paralysis virus (CrPV); the median survival after inoculation with 350 TCID₅₀ of CrPV is reduced compared to controls. (van Rij et al., 2006) Dcr-2^L811fsX flies show increased mortality and an increased level of viral accumulation following infection with both Flock house virus (FHV) and cricket paralysis virus (CrPV). (Wang et al., 2006) When Dcr-2^L811fsX germ-line clones are made a normal progression of egg chambers is seen. (Hatfield et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement Reference Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006)
Suppressor of
Statement Reference Dcr-2[+]/Dcr-2^L811fsX is a suppressor \| partially of increased cell death phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, th^{dsRNA.Scer\UAS} (Meyer et al., 2006) Dcr-2[+]/Dcr-2^L811fsX is a suppressor \| partially of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011) Dcr-2^L811fsX/Dcr-2^L811fsX is a suppressor of eye color defective phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Pelisson et al., 2007, Pelisson et al., 2007) Dcr-2^L811fsX is a suppressor \| somatic clone of eye color defective phenotype of w^IR.GMR (Zhou et al., 2008) Dcr-2^L811fsX is a suppressor of eye color defective phenotype of w^IR.GMR (Lim et al., 2008) Dcr-2^L811fsX is a suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012)
NOT Suppressor of
Statement Reference Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006)
Other
Statement Reference Dcr-1^Q1147X, Dcr-2^L811fsX has decreased cell number \| somatic clone \| larval stage phenotype (Shcherbata et al., 2007) Dcr-1^Q1147X, Dcr-2^L811fsX has decreased cell number \| somatic clone \| pupal stage phenotype (Shcherbata et al., 2007)
Phenotype Manifest In
Suppressed by
Statement Reference Dcr-2^L811fsX, w^IR.GMR has eye phenotype, suppressible \| partially by Dcr-2^E1371K.t7.2 (Lee et al., 2004) Dcr-2^L811fsX, w^IR.GMR has eye phenotype, suppressible \| partially by Dcr-2^E1617K.t7.2 (Lee et al., 2004) Dcr-2^L811fsX, w^IR.GMR has eye phenotype, suppressible by Dcr-2^E1210V.t7.2 (Lee et al., 2004) Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, suppressible \| partially by Dcr-2^E1371K.t7.2 (Lee et al., 2004) Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, suppressible \| partially by Dcr-2^E1617K.t7.2 (Lee et al., 2004) Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, suppressible by Dcr-2^E1210V.t7.2 (Lee et al., 2004)
NOT suppressed by
Statement Reference Dcr-2^L811fsX, w^IR.GMR has eye phenotype, non-suppressible by Dcr-2^{E1371K.E1617K.t7.2} (Lee et al., 2004) Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, non-suppressible by Dcr-2^{E1371K.E1617K.t7.2} (Lee et al., 2004)
NOT Enhancer of
Statement Reference Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006)
Suppressor of
Statement Reference Dcr-2[+]/Dcr-2^L811fsX is a suppressor \| partially of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2011) Dcr-2[+]/Dcr-2^L811fsX is a suppressor \| partially of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, th^{dsRNA.Scer\UAS} (Meyer et al., 2006) Dcr-2^L811fsX/Dcr-2^L811fsX is a suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Pelisson et al., 2007, Pelisson et al., 2007) Dcr-2^L811fsX/Dcr-2^L811fsX is a suppressor of wing phenotype of sd^Fab-X (Grimaud et al., 2006, Grimaud et al., 2006) Dcr-2^L811fsX is a suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Fukunaga et al., 2012) Dcr-2^L811fsX is a suppressor of eye phenotype of w^IR.GMR (Lee et al., 2004) Dcr-2^L811fsX is a suppressor of pigment cell phenotype of w^IR.GMR (Lee et al., 2004)
NOT Suppressor of
Statement Reference Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} (Lawlor et al., 2012) Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006) Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of retina phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bilen et al., 2006)
Other
Statement Reference Dcr-1^Q1147X, Dcr-2^L811fsX has female germline stem cell \| somatic clone \| larval stage phenotype (Shcherbata et al., 2007) Dcr-1^Q1147X, Dcr-2^L811fsX has female germline stem cell \| somatic clone \| pupal stage phenotype (Shcherbata et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference The eyes of w[dsRNA.GMR] transgenic female flies homozygous for Dcr-2[L811fsX] are similar to that of wild-type. (Fukunaga et al., 2012) w[IR.GMR] eyes that are also homozygous for Dcr-2[L811fsX] show strong suppression of the pale eye colour phenotype. (Lim et al., 2008) Flies carrying w[IR.GMR] in which the eyes are homozygous for Dcr-2[L811fsX] have dark red eyes. (Zhou et al., 2008) Flies carrying one copy of w[dsRNA.GMR] in a homozygous Dcr-2[L811fsX] background have red eyes. (Pelisson et al., 2007) Dcr-1[Q1147X] Dcr-2[L811fsX] double mutants show strong germline stem cell maintenance (GSC) defects when GSC clones are induced during late larval/early pupal stages. (Shcherbata et al., 2007) Eye degeneration due to expression of Hsap\MJD[tr.Q78.Scer\UAS.T:Ivir\HA1] in the eye (under the control of Scer\GAL4[GMR.PF]) is unaffected by a Dcr-2[L811fsX] background. (Bilen et al., 2006) The wing phenotype of sd^Fab-X flies is suppressed in sd^Fab-X;Dcr-2^L811fsX flies. (Grimaud et al., 2006) A Dcr-2^L811fsX/+ background partially suppresses the small eye phenotype that is seen when th^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF. Dcr-2^L811fsX; Dcr-1^Q1147X double mutants show no segment polarity defects. (Meyer et al., 2006)
Xenogenetic Interactions
Statement Reference Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] expression using Scer\GAL4[da.G32] in the presence of heterozygous Dcr-2[L811fsX] does not give significantly different phenotype severity compared to Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] expression in a wild-type Dcr-2 background. Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] expression using Scer\GAL4[da.G32] in the presence of heterozygous Dcr-2[L811fsX] does not give significantly different phenotype severity compared to Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] expression in a wild-type Dcr-2 background. (Lawlor et al., 2012) Heterozygous Dcr-2[L811fsX] suppresses the eye-phenotype resulting from the co-expression of Zzzz\CAG[99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] and Zzzz\CAG[rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF]. In addition, the overall disruption observed in patterning of the internal structure of the eye is significantly reduced in the presence of heterozygous Dcr-2[L811fsX] as revealed by sections through adult eyes. (Lawlor et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	32064 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; Dcr-2^L811fsX; P{Dcr-2^E1371K.t7.2}3 33053 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D Dcr-2^L811fsX/CyO; P{Dcr-2^+t7.2}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	dcr-2^L811Ex (Ashraf et al., 2006) dcr-2^L811fs (Martin et al., 2009) Dcr-2^L811Fsx (Czech et al., 2008, Mueller et al., 2010) dcr-2^L811fsX (Tomari et al., 2004, Matranga et al., 2005, Vagin et al., 2006, Pek and Kai, 2011, Lim and Kai, 2007, Förstemann et al., 2007, Tomari et al., 2007, Horwich et al., 2007, Bilen et al., 2006, Ghildiyal et al., 2008, Lim and Kai, 2007, Pelisson et al., 2007, Zhou et al., 2008, Pek and Kai, 2011, Lim et al., 2008, Fukunaga et al., 2012) Dcr-2^L811fsX (Grimaud et al., 2006, Wang et al., 2006, Wang et al., 2006, Vagin et al., 2006, Meyer et al., 2006, van Rij et al., 2006, Galiana-Arnoux et al., 2006, Okamura et al., 2008, Poulton et al., 2011, Lawlor et al., 2012) dcr2^L811fsX (Dufourt et al., 2011, Lawlor et al., 2011, Fagegaltier et al., 2009, Okamura et al., 2011) Dcr-2^L811FSX (Deddouche et al., 2008) Dcr-2^L811fsx (Habayeb et al., 2009) Dcr-2^L811X (Hatfield et al., 2005) dcr-2^L811X (Shcherbata et al., 2007) dicer-2^L811fsX (Harris et al., 2011) Dicer-2^L811fsX (Berdnik et al., 2008)
Name Synonym	Suppressor(GMR-wIR)A293 (Lee et al., 2004)
Secondary FlyBase IDs

References ( 43 )

Generate a list of	All references relating to this allele ( 43 )

List References by type	Research paper ( 40 ) Supplementary material ( 2 ) Erratum ( 1 )
Recent research papers ( 9 )
	Fukunaga et al., 2012, Cell 151(3): 533--546 Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. [FBrf0219804] Lawlor et al., 2012, PLoS ONE 7(6): e38516 Ubiquitous Expression of CUG or CAG Trinucleotide Repeat RNA Causes Common Morphological Defects in a Drosophila Model of RNA-Mediated Pathology. [FBrf0218612] Dufourt et al., 2011, DNA Res. 18(6): 451--461 Polycomb group-dependent, heterochromatin protein 1-independent, chromatin structures silence retrotransposons in somatic tissues outside ovaries. [FBrf0216699] Harris et al., 2011, J. Cell Biol. 194(1): 77--87 Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing. [FBrf0214287] Lawlor et al., 2011, Hum. Mol. Genet. 20(19): 3757--3768 Double-stranded RNA is pathogenic in Drosophila models of expanded repeat neurodegenerative diseases. [FBrf0215240] Okamura et al., 2011, Mol. Cell. Biol. 31(4): 884--896 R2D2 Organizes Small Regulatory RNA Pathways in Drosophila. [FBrf0212879] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Pek and Kai, 2011, Curr. Biol. 21(1): 39--44 A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718] Poulton et al., 2011, Development 138(9): 1737--1745 The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. [FBrf0213494] Show all research papers ...

Allele Dmel\Dcr-2L811fsX

Allele Dmel\Dcr-2^L811fsX