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General Information
Symbol
Dmel\Dcr-2L811fsX
Species
D. melanogaster
Name
FlyBase ID
FBal0156733
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dcr2L811fsX, dicer-2L811fsX, Dcr-2L811X, dicer-2null
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:
Leu 811 is replaced by FGIRSLCWIVAARRTPTWX.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
The Leucine at residue 811 is replaced by FGIRSLCWIVAARRTPTWX.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Dcr-2L811fsX homozygotes do not show significant differences in viral load in the head upon infection with ZIKV, as compared to infected controls.
The fertility of Dcr-2L811fsX/Df(2R)BSC45 mutant males is severely reduced compared to wild-type controls (female fertility is not affected) due decreased number of mature sperm in their seminal vesicle. The spermatid nuclear alignment as well as the alignment of the investment cones in thee testes is disrupted.
The survival rate of DIP1EY02625 mutants after DCV infection is 17% compared to controls. Median survival is 5-6 days.
Compared with DCV-infected wild-type flies, Dcr-2L811fsX homozygotes have a much higher viral load.
Dcr-2L811fsX/Df(2R)BSC45 flies injected with Invertebrate Iridescent Virus type 6 (IIV-6) die earlier than IIV-6 infected wild-type controls. A fraction of Dcr-2L811fsX/Df(2R)BSC45 flies injected with buffer also die in the course of the experiment, indicating an increased sensitivity to stress associated with the mutation.
Dcr-2L811fsX mutant flies are more susceptible to non-virulent Flock house virus (FHV) mutant infection than wild-type. The FHV mutant that lacks viral protein FHV\B2, a known RNAi suppressor, is not pathogenic in wild-type flies, but Dcr-2L811fsX mutants show decreased survival after infection. Unlike the case in wild-type flies, the FHV FHV\B2 mutant replicates to high levels in Dcr-2L811fsX mutants.
More than 60% of cells in anaphase mutant wing discs contain lagging chromosomes.
Dcr-2L811fsX/+ significantly suppresses the position effect variegation of the Sb1 allele that is seen in the T(2;3)SbV chromosome. Dcr-2L811fsX/Dcr-2R416X suppresses the position effect variegation of the w gene that is seen in the In(1)wm4 chromosome.
Homozygous projection neurons show normal dendrite and axon targeting.
Dcr-2L811fsX mutant germline stem cells (GSCs) do not exhibit any maintenance defects when clones are induced either during larval/pupal stages or during adult stages.
Mutant flies are more susceptible to infection with RNA viruses (Flock House virus, Drosophila C virus or Sindbis virus) than control flies.
Embryos derived from Dcr-2L811fsX mothers exhibit normal cellularization and net lipid-droplet transport.
Dcr-2L811fsX homozygous flies fail to produce siRNA from a transfected construct.
Dcr-2L811fsX flies show increased mortality and an increased level of viral accumulation following infection with both Flock house virus (FHV) and cricket paralysis virus (CrPV).
Mutant flies are hypersensitive to infection with Cricket Paralysis virus (CrPV); the median survival after inoculation with 350 TCID50 of CrPV is reduced compared to controls.
When Dcr-2L811fsX germ-line clones are made a normal progression of egg chambers is seen.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Dcr-2L811fsX heterozygosity partially suppresses the mortality of flamA, ctA double homozygotes.
The eyes of wdsRNA.GMR transgenic female flies homozygous for Dcr-2L811fsX are similar to that of wild-type.
A Dcr-2L811fsX homozygous mutant background suppresses the developmental lethality associated with expression of Disc\RFP(CTG)130.Scer\UAS and Disc\RFPCAG100.DsRed2.Scer\UAS in the developing muscle (under the control of Scer\GAL4how-24B). A Dcr-2L811fsX homozygous mutant background suppresses the organismal lethality associated with 30 minute heat-shock induced expression of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS (under the control of Scer\GAL4hs.PB).
wIR.GMR eyes that are also homozygous for Dcr-2L811fsX show strong suppression of the pale eye colour phenotype.
Flies carrying wIR.GMR in which the eyes are homozygous for Dcr-2L811fsX have dark red eyes.
Flies carrying one copy of wdsRNA.GMR in a homozygous Dcr-2L811fsX background have red eyes.
Dcr-1Q1147X Dcr-2L811fsX double mutants show strong germline stem cell maintenance (GSC) defects when GSC clones are induced during late larval/early pupal stages.
Eye degeneration due to expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF) is unaffected by a Dcr-2L811fsX background.
The wing phenotype of sdFab-X flies is suppressed in sdFab-X;Dcr-2L811fsX flies.
A Dcr-2L811fsX/+ background partially suppresses the small eye phenotype that is seen when thdsRNA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF. Dcr-2L811fsX; Dcr-1Q1147X double mutants show no segment polarity defects.
Xenogenetic Interactions
Statement
Reference
Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression using Scer\GAL4da.G32 in the presence of heterozygous Dcr-2L811fsX does not give significantly different phenotype severity compared to Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression in a wild-type Dcr-2 background. Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression using Scer\GAL4da.G32 in the presence of heterozygous Dcr-2L811fsX does not give significantly different phenotype severity compared to Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression in a wild-type Dcr-2 background.
Heterozygous Dcr-2L811fsX suppresses the eye-phenotype resulting from the co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF. In addition, the overall disruption observed in patterning of the internal structure of the eye is significantly reduced in the presence of heterozygous Dcr-2L811fsX as revealed by sections through adult eyes.
Heterozygosity for Dcr-2L811fsX in a Scer\GAL4Act5C.PI Zzzz\repScer\UAS.T:Avic\GFP background results in increased Sindbis viral replication.
Complementation and Rescue Data
Comments
The severely reduced fertility of Dcr-2L811fsX/Df(2R)BSC45 mutant males along with their sperm deficiency is rescued by combination with Dcr-2+tKa.
Dcr-2+tKa rescues the increased susceptibility of Dcr-2L811fsX/Df(2R)BSC45 mutant flies to IIV-6 infection.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (18)
Reported As
Symbol Synonym
Dcr-2L811fsx
Dicer-2L811fsX
dcr-2L811Ex
dcr-2L811fs
dicer-2L811fsX
dicer2L811fsX
Name Synonyms
Suppressor(GMR-wIR)A293
Secondary FlyBase IDs
    References (63)