FlyBase Allele Report: Dmel\Dcr-1[Q1147X]

One copy of Dcr-1^Q1147X increases the survival rates of both males and female adult flies subjected to nutrient starvation when compared to control flies. The levels of triacylglycerides (TAGs) and glycogen are increased at the beginning of starvation and the consumption of energy resources is delayed with time.

(Barrio et al., 2014)

Homozygous Dcr-1^Q1147X adult mushroom body clones display a reduction in cell number and absence of α'/β' and α/β neurons.

Homozygous anterodorsal projection neuron (adPN) clones show a reduction in cell number compared to controls when analysed at the adult stage. This cell number defect is not seen in homozygous adPN clones in which only early larval born adPNs are marked.

(Luhur et al., 2014)

7 days old females carrying homozygous germline clones result in egg chambers with 8 nurse cells and no oocyte with 37% penetrance.

(Azzam et al., 2012)

Flies homozygous for Dcr-1^Q1147X are not viable.

(Lawlor et al., 2012)

Dcr-1^19E2/Dcr-1^Q1147X and Dcr-1^18E6/Dcr-1^Q1147X results in pharate adults with normal morphology, although some adult escapers are seen.

(Smibert et al., 2011)

Wings containing homozygous clones are not flat and show blistering, but there is no loss of margin tissue.

Small homozygous clones in the wing disc contain apoptotic cells.

(Bejarano et al., 2010)

Expression of Dcr-1^VDRC.cUa driven by Scer\GAL4^ple.PF in a heterozygous Dcr-1^Q1147X genetic background results in dopaminergic neuron loss and climbing defects.

(Gehrke et al., 2010)

Dcr-1^Q1147X heterozygosity enhances the wing size defects caused by the expression of Scer\GAL4^en-e16E>Dcr-1^GD11429. The viability of these flies is drastically reduced.

Wing margin cells of Dcr-1^Q1147X mutant somatic clones display size defects compared to surrounding tissue.

Wing disc clones of Dcr-1^Q1147X mutant cells are on average smaller than their corresponding wild-type twin clones. These clones are frequently fragmented and many clones are eliminated from the wing disc by 96 h after induction. Most Dcr-1^Q1147X mutant clones show signs of apoptosis.

When mutant cells are given a relative growth advantage using the Minute technique using RpS3²/+ to impair growth of the other cells, Dcr-1^Q1147X mutant clones are recovered at the same frequency as wild-type control clones induced 96 h before dissection. However, Dcr-1^Q1147X mutant clones are much smaller than the wild-type control clones.

Dcr-1^Q1147X mutant wing disc cells in somatic clones display G[[1]]-S cell cycle delay.

(Herranz et al., 2010)

Homozygous clones result in wing blistering, external loss of notum bristles and small, rough eyes.

Homozygous clones contribute far less to the adult eye than their wild-type twin spots.

Homozygous clones induced in the eye disc are unable to eliminate Minute heterozygous

cells, in contrast to wild-type clones.

(Martin et al., 2009)

The low cell division index of homozygous female germline stem cell clones does not change under poor food conditions (in contrast to wild-type clones, where the cell division is dramatically reduced under poor food conditions).

(Yu et al., 2009)

Heterozygous females have normal ovary morphology and normal fertility.

(Kalidas et al., 2008)

Dcr-1^Q1147X MARCM clones do not exhibit a significantly reduced number of dendritic ends of ddaC neurons; however, terminal branches fail to elaborate properly.

(Xu et al., 2008)

Homozygous embryos do not develop past the first larval instar stage.

(Park et al., 2007)

Dcr-1^Q1147X mutant germline stem cells (GSCs) that have been generated during adult stages divide slowly compared to wild type, with mutant stem cells producing one progeny compared to 4 in controls. Dcr-1[Q1147X] mutant stem cells often leave the niche; if a wild type stem cell is also present in the niche it divides to replace the missing mutant, but if both of the GSCs present are mutant the niche is left empty. On average 12% of Dcr-1^Q1147X mutant GSCs are lost per day, compared to only 2% of control GSCs.

(Shcherbata et al., 2007)

Dcr-1^Q1147X mutants exhibit defects in eye morphology.

(Bilen et al., 2006)

Dcr-1^Q1147X flies show a modest but significant disruption of the heterochromatic silencing of the P{T1} reporter in line P{T1}T190-177 and of the P{T1(-FRT)} reporter in line P{T1(-FRT)}T190-177.

(Haynes et al., 2006)

70% of embryos produced by females with Dcr-1^Q1147X germline clones are devoid of pole cells and the remaining 32% contain ~16 pole cells. 37% of these embryos are arrested at embryonic cycle 1 or are unfertilized, and 9% are arrested before cycle 8. In addition, these embryos show severe defects in chromosomal segregation, nuclear distribution and supernumerary mitoses. Among mitotically normal embryos from Dcr-1^Q1147X mothers with nuclei present in the posterior pole region at cycle 9-14, 50% of them had no pole cells, and 22% contained fewer than ten pole cells.

(Megosh et al., 2006)

When Dcr-1^Q1147X germline clones are made in the ovarioles, many of the developmental stages are missing in the ovarioles. The proportion of germline stem cells (GSCs) remains close to normal in these clones. Mutant GSCs show normal spectrosome morphology and position. The frequency of cell division in mutant GSCs is reduced to 18% of normal levels. The number of these mutant GSCs that in S phase is also reduced, suggesting that the cell cycle is delayed at the G1/S transition. Affects in the cell cycle are also seen in male germline stem cells.

(Hatfield et al., 2005)

Dcr-1^Q1147X oocytes are ventralised.

(Nakahara et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dcr-1^Q1147X, w^IR.GMR has size defective phenotype, enhanceable by FBXO11^Q803R

(Lee et al., 2009)

Suppressed by

Statement

Reference

Dcr-1^Q1147X has starvation stress response defective phenotype, suppressible by p53^-ns.3'/p53^-ns.5'

(Barrio et al., 2014)

Dcr-1^Q1147X has starvation stress response defective phenotype, suppressible by p53[+]/p53^-ns

(Barrio et al., 2014)

Dcr-1^Q1147X has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by Myc^UAS.cZa/Scer\GAL4^αTub84B.PL

(Herranz et al., 2010)

Dcr-1^Q1147X, w^IR.GMR has eye color defective phenotype, suppressible by FBXO11^Q803R

(Lee et al., 2009)

Dcr-1^Q1147X has decreased occurrence of cell division | germline clone | oogenesis phenotype, suppressible | partially by dap[+]/dap⁴

(Hatfield et al., 2005)

Enhancer of

Statement

Reference

Dcr-1^Q1147X is an enhancer of majority die during first instar larval stage phenotype of Gas41^f05565

(Gandhi et al., 2015)

Dcr-1^Q1147X is an enhancer of melanotic necrosis | adult stage phenotype of Gas41^f05565

(Gandhi et al., 2015)

Dcr-1^Q1147X/Dcr-1[+] is an enhancer of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-1^Q1147X is an enhancer of increased cell death phenotype of Hsap\ATXN3^{tr.Q61.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Dcr-1^Q1147X, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Suppressor of

Statement

Reference

Dcr-1^Q1147X/Dcr-1[+] is a suppressor | partially of visible phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-1^Q1147X is a suppressor | partially | somatic clone of eye color defective phenotype of w^{miRNA.GUS.DsRed(T4)}

(Smibert et al., 2011)

Dcr-1^Q1147X is a suppressor | partially of eye color defective phenotype of w^IR.GMR

(Lee et al., 2009)

Dcr-1^Q1147X is a suppressor of neuroanatomy defective phenotype of Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa

(Xu et al., 2008)

Other

Statement

Reference

Dcr-1^Q1147X, w^IR.GMR has size defective phenotype

(Lee et al., 2009)

Dcr-1^Q1147X/Dcr-1[+], r2d2¹ has female sterile phenotype

(Kalidas et al., 2008)

Dcr-1^Q1147X/Dcr-1[+], Dcr-2^R416X has female fertile phenotype

(Kalidas et al., 2008)

Dcr-1^Q1147X, Mad¹²/Mad[+] has decreased cell number | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Mad¹²/Mad[+] has decreased cell number | somatic clone | pupal stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Dcr-2^L811fsX has decreased cell number | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Dcr-2^L811fsX has decreased cell number | somatic clone | pupal stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X/Dcr-1[+], Mad¹² has decreased cell number | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dcr-1^Q1147X, w^IR.GMR has eye phenotype, enhanceable by FBXO11^Q803R

(Lee et al., 2009)

Suppressed by

Statement

Reference

Dcr-1^Q1147X has wing disc | somatic clone | third instar larval stage phenotype, suppressible | partially by Myc^UAS.cZa/Scer\GAL4^αTub84B.PL

(Herranz et al., 2010)

Dcr-1^Q1147X, w^IR.GMR has eye phenotype, suppressible by FBXO11^Q803R

(Lee et al., 2009)

Dcr-1^Q1147X has germline stem cell | germline clone phenotype, suppressible | partially by dap[+]/dap⁴

(Hatfield et al., 2005)

Dcr-1^Q1147X has female germline cyst | germline clone | oogenesis phenotype, suppressible | partially by dap[+]/dap⁴

(Hatfield et al., 2005)

Enhancer of

Statement

Reference

Dcr-1^Q1147X is an enhancer of scutum phenotype of Gas41^f05565

(Gandhi et al., 2015)

Dcr-1^Q1147X is an enhancer of scutellum phenotype of Gas41^f05565

(Gandhi et al., 2015)

Dcr-1^Q1147X/Dcr-1[+] is an enhancer of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-1^Q1147X/Dcr-1[+] is an enhancer of egg chamber phenotype of r2d2¹

(Kalidas et al., 2008)

Dcr-1^Q1147X, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Dcr-1^Q1147X, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Dcr-1^Q1147X is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q61.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Dcr-1^Q1147X is an enhancer of retina phenotype of Hsap\ATXN3^{tr.Q61.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Suppressor of

Statement

Reference

Dcr-1^Q1147X/Dcr-1[+] is a suppressor | partially of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-1^Q1147X is a suppressor | partially | somatic clone of eye phenotype of w^{miRNA.GUS.DsRed(T4)}

(Smibert et al., 2011)

Dcr-1^Q1147X is a suppressor | partially of eye phenotype of w^IR.GMR

(Lee et al., 2009)

Dcr-1^Q1147X/Dcr-1[+] is a suppressor of male germline cell phenotype of mael^M391/Df(3L)79E-F

(Pek et al., 2009)

Dcr-1^Q1147X is a suppressor of abdominal 1 dorsal multidendritic neuron ddaC phenotype of Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa

(Xu et al., 2008)

Dcr-1^Q1147X is a suppressor of dendrite phenotype of Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa

(Xu et al., 2008)

Dcr-1^Q1147X is a suppressor of eye phenotype of w^IR.GMR

(Lee et al., 2004)

Dcr-1^Q1147X is a suppressor of pigment cell phenotype of w^IR.GMR

(Lee et al., 2004)

NOT Suppressor of

Statement

Reference

Dcr-1^Q1147X/Dcr-1[+] is a non-suppressor of wing phenotype of sd^Fab-X

(Grimaud et al., 2006)

Other

Statement

Reference

Dcr-1^Q1147X, w^IR.GMR has eye phenotype

(Lee et al., 2009, Lee et al., 2004)

Dcr-1^Q1147X, Mad¹²/Mad[+] has female germline stem cell | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Mad¹²/Mad[+] has female germline stem cell | somatic clone | pupal stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Dcr-2^L811fsX has female germline stem cell | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Dcr-2^L811fsX has female germline stem cell | somatic clone | pupal stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X/Dcr-1[+], Mad¹² has female germline stem cell | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

AGO1^k08121, Dcr-1^Q1147X has embryonic/first instar larval cuticle | segment polarity expression pattern phenotype

(Meyer et al., 2006)

Dcr-1^Q1147X, w^IR.GMR has interommatidial bristle phenotype

(Lee et al., 2004)

Dcr-1^Q1147X, w^IR.GMR has microchaeta phenotype

(Lee et al., 2004)

Dcr-1^Q1147X, w^IR.GMR has ommatidium phenotype

(Lee et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The mortality of Gas41^f05565 homozygotes as well as the necrotic patches (on scutum, notum and scutellum) observed in the surviving adults is further enhanced by combination with Dcr-1^Q1147X.

(Gandhi et al., 2015)

One copy of p53^-ns suppresses the increase in survival rate in response to starvation seen in adult Dcr-1^Q1147X/+ mutant flies.

(Barrio et al., 2014)

Expression of dm^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in Dcr-1^Q1147X mutant somatic clones completely suppresses cell loss, but clone size is not completely restored.

(Herranz et al., 2010)

The lighter colour of w^IR.GMR eyes is partially suppressed when eyes are also made homozygous mutant for Dcr-1^Q1147X (using the "eyFLP/FRT" system). w^IR.GMR, Dcr-1^Q1147X are also smaller than w^IR.GMR eyes.

w^IR.GMR eyes made homozygous for both FBX011^Q803R and Dcr-1^Q1147X (using the "eyFLP/FRT" system) show a greater suppression of the eye pigmentation phenotype but an enhancement of the small eye phenotype seen in w^IR.GMR, Dcr-1^Q1147X eyes.

(Lee et al., 2009)

The differentiation defects seen in the testes of mael^M391/Df(3L)79E-F males are partially rescued by Dcr-1^Q1147X/+.

(Pek et al., 2009)

The female fertility defect seen in r2d2¹ females is dramatically enhanced by Dcr-1^Q1147X/+; the females are completely sterile and the ovaries are severely disorganised with completely fused follicles.

Dcr-2^R416X ; Dcr-1^Q1147X/+ females have normal ovaries and normal fertility.

(Kalidas et al., 2008)

Ectopic expression of mir-124^Scer\UAS.cXa in homozygous Dcr-1^Q1147X ddaC neuron clones (under the control of Scer\GAL4⁴⁷⁷) does not affect the number of dendritic ends, indicating Dcr-1 suppression of mir-124 function.

(Xu et al., 2008)

Dcr-1^Q1147X mutant germline stem cells lacking one copy of Mad¹² show clear stem cell maintenance defects when clones are generated during late larval/early pupal stages.

Mad¹² mutant germline stem cells lacking one copy of Dcr-1^Q1147X show clear stem cell maintenance defects when clones are generated during late larval/early pupal stages.

Dcr-1^Q1147X Dcr-2^L811fsX double mutants show strong germline stem cell maintenance (GSC) defects when GSC clones are induced during late larval/early pupal stages.

(Shcherbata et al., 2007)

Eye degeneration due to expression of Hsap\ATXN3^{tr.Q61.Scer\UAS.T:Ivir\HA1} in the eye (under the control of Scer\GAL4^GMR.PF) is enhanced in a Dcr-1^Q1147X background, resulting in a severely degenerated eye with complete loss of pigmentation.

(Bilen et al., 2006)

The wing phenotype of sd^Fab-X flies is still present in sd^Fab-X;Dcr-1^Q1147X/+ flies.

(Grimaud et al., 2006)

Embryos from AGO1^k08121; Dcr-1^Q1147X parents show segment polarity defects, a phenotype not seen in either single mutant. Dcr-2^L811fsX; Dcr-1^Q1147X double mutants show no segment polarity defects.

(Meyer et al., 2006)

When dap⁴/+ and homozygous Dcr-1^Q1147X are combined a partial rescue of the cyst production phenotype seen Dcr-1^Q1147X mutants is seen.

(Hatfield et al., 2005)

Dcr-1^Q1147X, w^IR.GMR animals have eyes that have reduced pigment, and are also reduced in size. The organisation of ommatidial facets is also disrupted and sensory bristles are missing over the eye surface. Other bristles, which flank the eye surface, are sometimes absent or exhibit hyperplasia.

(Lee et al., 2004)

Xenogenetic Interactions

Statement

Reference

One of two lines expressing Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} using Scer\GAL4^da.G32 show a more severe tergite phenotype in the presence of heterozygous Dcr-1^Q1147X than when Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} is expressed alone.

In one of two lines expressing Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} using Scer\GAL4^da.G32 in the presence of heterozygous Dcr-1^Q1147X the proportion of progeny with any tergite-disruption phenotype, and those with a strong phenotype, is significantly reduced compared with flies expressing Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} alone.

(Lawlor et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32066

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}82B Dcr-1^Q1147X/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Dcr-1^Q1147X

(Eyk et al., 2019, Hanyu-Nakamura et al., 2019, Lo et al., 2016, Luhur et al., 2014, Lawlor et al., 2012, Poulton et al., 2011, Smibert et al., 2011, Habayeb et al., 2009, Yu et al., 2009, Grimaud et al., 2006, Megosh et al., 2006, Megosh et al., 2006, Meyer et al., 2006)

dcp-1^Q1147X

(Park et al., 2007)

dcr-1^Q1147X

(Fic et al., 2019, Barrio et al., 2014, Eun et al., 2013, Azzam et al., 2012, Gehrke et al., 2010, Herranz et al., 2010, Martin et al., 2009, Pek et al., 2009, Friggi-Grelin et al., 2008, Kalidas et al., 2008, Xu et al., 2008, Shcherbata et al., 2007, Bilen et al., 2006, Haynes et al., 2006, Hatfield et al., 2005, Nakahara et al., 2005)

dcr-1^Q1147x

(Bejarano et al., 2010, Lim and Kai, 2007, Lim and Kai, 2007)

dcr1^Q1147X

(Li and Baker, 2019, Gandhi et al., 2015, Cziko et al., 2009)

dcr1^Q1147x

(McCann et al., 2011)

Name Synonyms

Suppressor(GMR-wIR)D14

(Lee et al., 2004)

Secondary FlyBase IDs

References (39)

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