Allele Dmel\Dcp-1^Prev1

General Information
Symbol	Dmel\Dcp-1^Prev1	Species	D. melanogaster
Name		FlyBase ID	FBal0156746
Feature type	allele	Associated gene	Dmel\Dcp-1
Also Known As	Dcp-1^Prev

Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Laundrie et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Dcp-1^k05606 (Laundrie et al., 2003)
Nature of the lesion	Statement Reference Imprecise excision of P{lacW}Dcp-1^k05606 has left 40bp of P-element sequence at the insertion site leading to a frameshift in Dcp-1 and an in-frame stop codon within the 40-bp insertion. (Laundrie et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	cell death defective (Lee et al., 2011) decreased cell death (Kondo et al., 2006, Florentin and Arama, 2012) visible (Mendes et al., 2006)
Phenotype Manifest In
	chaeta \| supernumerary (Mendes et al., 2006) dendrite (Schoenmann et al., 2010) egg chamber \| conditional (Laundrie et al., 2003) eye disc (Kondo et al., 2006) neuron (Lee et al., 2011)
Detailed Description
	Statement Reference Dcp-1[Prev1] mutants display a significant delay of ~1hr in the rate of cell death, reaching the wild-type plateau level 4hrs after irradiation. Dcp-1[Prev1] mutants do not exhibit a difference in survival rate compared to wild-type controls. (Florentin and Arama, 2012) Homozygous Dcp-1[Prev1] mutants are viable and do not display any defects in NMJ integrity or growth. (Keller et al., 2011) Dcp-1[Prev1] mutants exhibit an average of 9 vCrz somata at 7 hours after puparium formation, with no vCrz somata at 16 hours after puparium formation. The general morphology of vCrz neurons at 7 hours after puparium formation was comparable to those of wild-type at 4-5 hours after puparium formation, indicating that programmed cell death has been slowed down in the absence of Dcp-1 function. (Lee et al., 2011) Dcp-1[Prev1] mutants exhibit a mild reduction in dendritic branch pruning at 18 hours after puparium formation. (Schoenmann et al., 2010) Developmental apoptosis is significantly reduced in homozygous third instar eye discs. γ-ray induced apoptosis is not suppressed in homozygous third instar wing discs. (Kondo et al., 2006) The arista of Dcp-1^Prev1 flies appears wild type. Dcp-1^Prev1 pupal eyes appear wild type. (Muro et al., 2006) Dcp-1^Prev1 homozygotes are viable and fertile. The ovaries of these flies are largely normal, but have occasional egg chambers lacking follicle cells. This phenotype is exacerbated by nutrient deprivation to a much greater degree than in wild-type. Degeneration of egg chambers correlates with ectopic expression of cell-death markers. (Laundrie et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Dcp-1^Prev1 has cell death defective phenotype, enhanceable by Nc^I29 (Lee et al., 2011)
NOT suppressed by
Statement Reference Dcp-1^Prev1 has decreased cell death phenotype, non-suppressible by Ice^Δ1 (Florentin and Arama, 2012)
Enhancer of
Statement Reference Dcp-1[+]/Dcp-1^Prev1 is an enhancer of lethal \| pupal stage phenotype of Ice^Δ1 (Muro et al., 2006) Dcp-1^Prev1 is an enhancer \| germline clone of decreased cell death \| embryonic stage phenotype of Ice¹⁷ (Xu et al., 2006) Dcp-1^Prev1 is an enhancer of cell death defective phenotype of Nc^I29 (Lee et al., 2011) Dcp-1^Prev1 is an enhancer of developmental rate defective \| pupal stage phenotype of Ice^Δ1 (Muro et al., 2006)
Suppressor of
Statement Reference Dcp-1[+]/Dcp-1^Prev1 is a suppressor \| partially of neuroanatomy defective phenotype of Ank2^f02001 (Keller et al., 2011) Dcp-1^Prev1 is a suppressor of neuroanatomy defective phenotype of Ank2^f02001 (Keller et al., 2011)
NOT Suppressor of
Statement Reference Dcp-1^Prev1/Dcp-1^Prev1 is a non-suppressor \| somatic clone of visible phenotype of W^GMR.PG/W^GMR.PG (Kondo et al., 2006, Kondo et al., 2006) Dcp-1^Prev1 is a non-suppressor of decreased cell death phenotype of Ice^Δ1 (Florentin and Arama, 2012) Dcp-1^Prev1 is a non-suppressor of partially lethal - majority die \| larval stage phenotype of Ice^Δ1 (Florentin and Arama, 2012)
Other
Statement Reference Dcp-1^Prev1, Ice¹⁷ has cell death defective \| germline clone phenotype (Baum et al., 2007) Dcp-1^Prev1, Ice^Δ1 has developmental rate defective \| pupal stage phenotype (Lee et al., 2011) Dcp-1^Prev1, Ice^Δ1 has lethal \| pharate adult stage phenotype (Lee et al., 2011)
Phenotype Manifest In
Enhanced by
Statement Reference Dcp-1^Prev1 has neuron phenotype, enhanceable by Nc^I29 (Lee et al., 2011)
Enhancer of
Statement Reference Dcp-1[+]/Dcp-1^Prev1 is an enhancer of arista phenotype of Ice^Δ1 (Muro et al., 2006) Dcp-1[+]/Dcp-1^Prev1 is an enhancer of male genitalia phenotype of Ice^Δ1 (Muro et al., 2006) Dcp-1^Prev1 is an enhancer of neuron phenotype of Nc^I29 (Lee et al., 2011)
Suppressor of
Statement Reference Dcp-1[+]/Dcp-1^Prev1 is a suppressor \| partially of embryonic/larval neuromuscular junction phenotype of Ank2^f02001 (Keller et al., 2011) Dcp-1^Prev1 is a suppressor of embryonic/larval neuromuscular junction phenotype of Ank2^f02001 (Keller et al., 2011)
NOT Suppressor of
Statement Reference Dcp-1^Prev1/Dcp-1^Prev1 is a non-suppressor \| somatic clone of eye phenotype of W^GMR.PG/W^GMR.PG (Kondo et al., 2006, Kondo et al., 2006) Dcp-1^Prev1 is a non-suppressor of wing disc phenotype of Ice^Δ1 (Florentin and Arama, 2012)
Other
Statement Reference Dcp-1^Prev1, Ice¹⁷ has egg chamber \| germline clone phenotype (Baum et al., 2007) Dcp-1^Prev1, Ice^Δ1 has neuron phenotype (Lee et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Dcp-1[Prev1]; Ice[Δ1] double mutants exhibit a block on cell death after irradiation. A Dcp-1[Prev1]; Ice[Δ1] double mutant background fails to abolish the ability of Ice[fl.5'3'UTR] mutants to induce cell death, although induction is at a reduced rate compared to in a Ice[Δ1] single mutant background. (Florentin and Arama, 2012) Homozygous Dcp-1[Prev1] suppresses the NMJ degeneration seen in Ank2[f02001] mutants. One copy of Dcp-1[Prev1] is also able to partially suppress the phenotype. (Keller et al., 2011) Dcp-1[Prev1]; Nc[I29] double mutants exhibit an arrest of vCrz programmed cell death with all 16 vCrz neurons retained at 7 and 16 hours after puparium formation, compared to apoptosis of these neurons in wild-type controls. (Lee et al., 2011) Dcp-1[Prev1] ; Nc[I24]/Nc[I29] Ice[Δ1] triple mutant larvae undergo cell death with morphology similar to the midgut of wild-type animals when analysed from -4 to -1 hours relative to puparium formation. (Denton et al., 2009) Females containing Dcp-1[Prev1] Ice[17] double mutant germline clones have persisting nurse cell nuclei in 21% of stage 14 egg chambers. (Baum et al., 2007) Developmental apoptosis in the eye disc is completely absent in Dcp-1[Prev1] ; Ice[Δ2C8] double homozygous third instar larvae. The reduction in adult eye size caused by expression of W[GMR.PG] is not suppressed if the eye is made homozygous for Dcp-1[Prev1] (using the eyFLP method). (Kondo et al., 2006) The lethality of Ice^Δ1 is enhanced by Dcp-1^Prev1: less than 1% of Dcp-1^Prev1/+, Ice^Δ1/Ice^Δ1 animals eclose and Dcp-1^Prev1/Dcp-1^Prev1; Ice^Δ1/Ice^Δ1 animals die prior to, or during, early pupal stages. Almost all Dcp-1^Prev1/Dcp-1^Prev1; Ice^Δ1/Ice^Δ1 pupae arrest development prior to head inversion. Dcp-1^Prev1/+, Ice^Δ1 flies show an increased number of lateral branches on the arista compared to Ice^Δ1 single mutant flies. Additionally, the genitalia misrotation phenotype of Ice^Δ1 flies is increased to full penetrance in Dcp-1^Prev1/+, Ice^Δ1 flies. (Muro et al., 2006) Ice[17] Dcp-1[Prev1] double mutant embryos derived from mutant female germline clones (so lacking maternal and zygotic function of Ice and Dcp-1 show significantly reduced levels of apoptosis compared to Ice[17] single maternal and zygotic mutant embryos, containing only a few dying cells. (Xu et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Dcp-1^Prev1 is rescued by Dcp-1^t4.4 (Laundrie et al., 2003)
Rescues	Dcp-1^Prev1 rescues Dcp-1^Ice.5'3'UTR (Florentin and Arama, 2012)
Comments	A Dcp-1[Prev1] heterozygous or homozygous background abolishes the ability of Dcp-1[Ice.5'3'UTR] mutants to induce cell death. (Florentin and Arama, 2012) The presence of egg chambers lacking follicle cells in nutrient deprived Dcp-1^Prev1 homozygotes is prevented by Dcp-1^t4.4. (Laundrie et al., 2003)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	Dcp-1^Prev1 (Lee et al., 2011) dcp-1^Prev1 (Muro et al., 2006, Xu et al., 2006, Denton et al., 2009, Keller et al., 2011, Baum et al., 2007) dcp-1^prev1 (Mendes et al., 2006, Kondo et al., 2006) dcp1^Prev1 (Amarneh et al., 2009) Dcp-1^Prev (Schoenmann et al., 2010) Dcp-1^prev (Florentin and Arama, 2012) ΔDcp-1 (Muro et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Florentin and Arama, 2012, J. Cell Biol. 196(4): 513--527 Caspase levels and execution efficiencies determine the apoptotic potential of the cell. [FBrf0217486] Keller et al., 2011, Neuron 72(5): 760--775 Glial-derived prodegenerative signaling in the Drosophila neuromuscular system. [FBrf0216904] Lee et al., 2011, J. Comp. Neurol. 519(1): 34--48 Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis. [FBrf0212368] Schoenmann et al., 2010, J. Neurosci. 30(18): 6375--6386 Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals. [FBrf0210748] Amarneh et al., 2009, J. Biol. Chem. 284(15): 9674--9682 Activation of sterol regulatory element-binding protein by the caspase Drice in Drosophila larvae. [FBrf0207690] Denton et al., 2009, Curr. Biol. 19(20): 1741--1746 Autophagy, not apoptosis, is essential for midgut cell death in Drosophila. [FBrf0209243] Baum et al., 2007, Cell Death Differ. 14(8): 1508--1517 The Drosophila caspases Strica and Dronc function redundantly in programmed cell death during oogenesis. [FBrf0201400] Kondo et al., 2006, Mol. Cell. Biol. 26(19): 7258--7268 DRONC coordinates cell death and compensatory proliferation. [FBrf0193775] Mendes et al., 2006, EMBO Rep. 7(9): 933--939 Cytochrome c-d regulates developmental apoptosis in the Drosophila retina. [FBrf0194789] Muro et al., 2006, Development 133(17): 3305--3315 The Drosophila caspase Ice is important for many apoptotic cell deaths and for spermatid individualization, a nonapoptotic process. [FBrf0192459] Xu et al., 2006, Cell Death Differ. 13(10): 1697--1706 The effector caspases drICE and dcp-1 have partially overlapping functions in the apoptotic pathway in Drosophila. [FBrf0193497] Laundrie et al., 2003, Genetics 165(4): 1881--1888 Germline cell death is inhibited by P-element insertions disrupting the dcp-1/pita nested gene pair in Drosophila. [FBrf0167648]

Allele Dmel\Dcp-1Prev1

Allele Dmel\Dcp-1^Prev1