FlyBase Allele Report: Dmel\CASK[UAS.cBa]

General Information

Symbol

Dmel\CASK^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Bryant

FlyBase ID

FBal0156806

Feature type

allele

Associated gene

Dmel\CASK

Associated Insertion(s)

Carried in Construct

P{UAS-Cmg.B}

Also Known As

uas-CASK, UAS-CASK-β

Key Links

Transgenic product class

cDNA

(Slawson et al., 2011)

wild_type

(Slawson et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Slawson et al., 2011)

wild_type

(Slawson et al., 2011)

Mutagen

in vitro construct

(Lu et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Cmg.B}

Cytology

Description

UAS drives expression of a CASK cDNA that corresponds to the CASK-RB mRNA encoding the CASK-β isoform.

(Slawson et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

CASK

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning, with Scer\GAL4^Tab2-201Y

(Gillespie and Hodge, 2013)

abnormal locomotor behavior, with CASK^β-null, Scer\GAL4^C164

(Slawson et al., 2011)

abnormal memory, with Scer\GAL4^c305a

(Malik et al., 2013)

abnormal memory, with Scer\GAL4^ey-OK107

(Malik et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mef2.PR

(Gillespie and Hodge, 2013)

Phenotype Manifest In

type Ib terminal bouton | third instar larval stage, with Scer\GAL4^Mef2.PR

(Gillespie and Hodge, 2013)

Detailed Description

Statement

Reference

Expression of CASK^Scer\UAS.cBa under the control of Scer\GAL4^VGlut-OK371 has no significant effect on either bouton number/area or bouton area for type Ib boutons at the larval neuromuscular junction.

Expression of CASK^Scer\UAS.cBa under the control of Scer\GAL4^Mef2.PR results in an increase in bouton number/area for type Ib boutons at the larval neuromuscular junction, while bouton area normal.

Flies expressing CASK^Scer\UAS.cBa under the control of Scer\GAL4^Tab2-201Y show a reduced performance index in an appetitive associative learning assay compared to wild type.

(Gillespie and Hodge, 2013)

Flies expressing CASK^Scer\UAS.cBa under the control of either Scer\GAL4^ey-OK107 or Scer\GAL4^c305a show a severe reduction in middle-term memory (measured 3 hours after one cycle of training) in an olfactory aversive conditioning assay.

Flies expressing CASK^Scer\UAS.cBa under the control of Scer\GAL4^Mef2.247 show normal middle-term memory (measured 3 hours after one cycle of training) in an olfactory aversive conditioning assay.

Flies expressing CASK^Scer\UAS.cBa under the control of either Scer\GAL4^ey-OK107 or Scer\GAL4^c305a have severely reduced long-term memory (measured 24 hours after 5 cycles of spaced training) in an olfactory aversive conditioning assay.

Flies expressing CASK^Scer\UAS.cBa under the control of Scer\GAL4^Mef2.247 show normal long-term memory (measured 24 hours after 5 cycles of spaced training) in an olfactory aversive conditioning assay.

(Malik et al., 2013)

Expression of CASK^Scer\UAS.cBa using Scer\GAL4^C164 not only rescues all locomotor parameters that are deficient in CASK^P18 flies, but enhances locomotor activity above wild type levels.

(Slawson et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

CASK^UAS.cBa, Scer\GAL4^ey-OK107 has abnormal memory phenotype, suppressible by CaMKII^{T306A.T307A.UAS}, Scer\GAL4^ey-OK107

(Malik et al., 2013)

NOT suppressed by

Statement

Reference

CASK^UAS.cBa, Scer\GAL4^c305a has abnormal memory phenotype, non-suppressible by CaMKII^{T306A.T307A.UAS}, Scer\GAL4^c305a

(Malik et al., 2013)

Suppressor of

Statement

Reference

CASK^UAS.cBa, Scer\GAL4^VGlut1-OK371 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of CaMKII^T287D.UAS, Scer\GAL4^VGlut1-OK371

(Gillespie and Hodge, 2013)

CASK^UAS.cBa, Scer\GAL4^ey-OK107 is a suppressor of abnormal memory phenotype of CaMKII^{T306A.T307A.UAS}, Scer\GAL4^ey-OK107

(Malik et al., 2013)

NOT Suppressor of

Statement

Reference

CASK^UAS.cBa, Scer\GAL4^c305a is a non-suppressor of abnormal memory phenotype of CaMKII^{T306A.T307A.UAS}, Scer\GAL4^c305a

(Malik et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

CASK^UAS.cBa, Scer\GAL4^VGlut1-OK371 is a suppressor of type Ib terminal bouton | third instar larval stage phenotype of CaMKII^T287D.UAS, Scer\GAL4^VGlut1-OK371

(Gillespie and Hodge, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CASK^Scer\UAS.cBa suppresses the increase in bouton number/area seen for type Ib boutons at the neuromuscular junction in larvae expressing CaMKII^{T287D.Scer\UAS} under the control of Scer\GAL4^VGlut-OK371.

(Gillespie and Hodge, 2013)

Co-expression of CASK^Scer\UAS.cBa and CaMKII^{T306A.T307A.Scer\UAS} under the control of Scer\GAL4^ey-OK107 is sufficient to rescue the reduction in middle-term memory (measured 3 hours after one cycle of training) seen in an olfactory aversive conditioning assay when either CASK^Scer\UAS.cBa or CaMKII^{T306A.T307A.Scer\UAS} is expressed alone under the control of Scer\GAL4^ey-OK107.

Co-expression of CASK^Scer\UAS.cBa and CaMKII^{T306A.T307A.Scer\UAS} under the control of Scer\GAL4^c305a does not rescue the reduction in middle-term memory (measured 3 hours after one cycle of training) seen in an olfactory aversive conditioning assay when either CASK^Scer\UAS.cBa or CaMKII^{T306A.T307A.Scer\UAS} is expressed alone under the control of Scer\GAL4^c305a.

(Malik et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

CASK^UAS.cBa/Scer\GAL4^c305a rescues CASK^β-null

(Malik et al., 2013)

CASK^UAS.cBa/Scer\GAL4^C164 rescues CASK^β-null

(Slawson et al., 2011)

Partially rescues

Scer\GAL4^elav-C155/CASK^UAS.cBa partially rescues CASK^β-null

(Tello et al., 2023)

Scer\GAL4^elav-C155/CASK^UAS.cBa partially rescues CASK^β-null

(Slawson et al., 2011)

Fails to rescue

Scer\GAL4^VGlut1-OK371/CASK^UAS.cBa fails to rescue CASK^β-null

(Slawson et al., 2011)

Comments

Expression of CASK^Scer\UAS.cBa under the control of Scer\GAL4^c305a fully rescues the reduction in middle-term memory (measured 3 hours after one cycle of training) seen in CASK^β-null flies in an olfactory aversive conditioning assay.

(Malik et al., 2013)

Expression of CASK^Scer\UAS.cBa using Scer\GAL4^elav-C155 partially rescues all locomotor parameters that are deficient in CASK^P18 flies.

Expression of CASK^Scer\UAS.cBa using Scer\GAL4^C164 fully rescues all locomotor parameters that are deficient in CASK^P18 flies.

Expression of CASK^Scer\UAS.cBa using Scer\GAL4^VGlut-OK371 fails to rescues the locomotor parameters that are deficient in CASK^P18 flies.

(Slawson et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CASK^Scer\UAS.cBa

CASK^UAS.cBa

Caki^Scer\UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Bryant

(Lu et al., 2003)

Secondary FlyBase IDs

References (6)

Report Sections

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