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General Information
Symbol
Dmel\Atg8aKG07569
Species
D. melanogaster
Name
FlyBase ID
FBal0157024
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Atg8a2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion in codon 28 of the open reading frame.

Insertion components
P{SUPor-P}Atg8aKG07569
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Atg8aKG07569 adults are much more sensitive to paraquat-induced oxidative stress than controls.

Atg8aKG07569 homozygous adult midguts do not show significant changes in the number of pre-enteroendocrine cells, as compared to heterozygous or wild-type controls.

Atg8aKG07569 mutant third instar larvae display highly increased number of lipid droplets in prothoracic gland cells.

Atg8aKG07569 mutants display significantly delayed midgut degradation after pupariation, with significantly increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, and significantly decreased autophagy levels 2 hrs after puparium formation, as compared to controls.

Autophagy is significantly decreased in Atg8aKG07569 mutant fat body cells.

18% of Atg8aKG07569 mutant pupae contain melanotic masses, such cell masses are not observed in Atg8aKG07569 mutant larvae.

Salivary glands appear normal in 6 hour mutant prepupae. However, at 24 hours after puparium formation, the mutant animals still contain salivary gland tissue (containing vacuolated salivary gland fragments), in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.

DNA fragmentation is detected by TUNEL in salivary glands of Atg8aKG07569 animals at 13.5 hours after puparium formation, as occurs in control salivary glands at this stage.

Starvation-induced autophagy is significantly reduced in mutant animals.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The induction of autophagy caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4hs.PH is significantly suppressed by Atg8aKG07569.

The elimination of cells expressing Atg1Scer\UAS.cSa under the control of Scer\GAL4Act5C.PP from the wing disc is significantly reduced in a Atg8aKG07569 background.

Xenogenetic Interactions
Statement
Reference

Co-expression of CalpAKK104532 in an Atg8aKG07569 mutant genetic background fails to reduce the number of aggregates observed in the eyes of flies expressing Scer\GAL4GMR.PF>Hsap\HTTQ46.ex1p.Scer\UAS.T:Avic\GFP-EGFP.

Co-expression of CalpAKK104532 in an Atg8aKG07569 mutant genetic background exacerbates the toxicity of Hsap\HTTGMR.Q120 expression in the eye.

Co-expression of CalpAKK104532 in an Atg8aKG07569 mutant genetic background fails to suppress the toxicity of Scer\GAL4GMR.PF>Hsap\MAPTScer\UAS.cWa.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (22)