Atg8aKG07569 adults are much more sensitive to paraquat-induced oxidative stress than controls.
Atg8aKG07569 homozygous adult midguts do not show significant changes in the number of pre-enteroendocrine cells, as compared to heterozygous or wild-type controls.
Atg8aKG07569 mutant third instar larvae display highly increased number of lipid droplets in prothoracic gland cells.
Atg8aKG07569 mutants display significantly delayed midgut degradation after pupariation, with significantly increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, and significantly decreased autophagy levels 2 hrs after puparium formation, as compared to controls.
Autophagy is significantly decreased in Atg8aKG07569 mutant fat body cells.
18% of Atg8aKG07569 mutant pupae contain melanotic masses, such cell masses are not observed in Atg8aKG07569 mutant larvae.
Salivary glands appear normal in 6 hour mutant prepupae. However, at 24 hours after puparium formation, the mutant animals still contain salivary gland tissue (containing vacuolated salivary gland fragments), in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.
DNA fragmentation is detected by TUNEL in salivary glands of Atg8aKG07569 animals at 13.5 hours after puparium formation, as occurs in control salivary glands at this stage.
Starvation-induced autophagy is significantly reduced in mutant animals.