FlyBase Allele Report: Dmel\tyn[PG38]

General Information

Symbol

Dmel\tyn^PG38

Species

D. melanogaster

Name

FlyBase ID

FBal0157146

Feature type

allele

Associated gene

Dmel\tyn

Associated Insertion(s)

P{GawB}tyn^PG38

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Bourbon et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{GawB}tyn^PG38

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Fernandes et al., 2010)

lethal | recessive

(Bourbon et al., 2002)

majority die during larval stage

(Wang et al., 2018)

Phenotype Manifest In

denticle

(Fernandes et al., 2010)

embryonic/larval spiracle | embryonic stage

(Wang et al., 2018)

embryonic/larval spiracle | larval stage

(Wang et al., 2018)

larval tracheal system | embryonic stage

(Wang et al., 2018)

larval tracheal system | larval stage

(Wang et al., 2018)

Detailed Description

Statement

Reference

Each denticle row is present, but the denticles themselves have a flattened base and thin extensions, pointing perpendicularly to the apical surface.

tyn^PG38 embryos show very basal defects in membrane/cuticle interactions.

tyn^PG38 embryos show an enlargement of the apical surface of denticle cells - smooth cells are not affected.

(Fernandes et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tyn^PG38 has lethal phenotype, suppressible by neo^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has lethal phenotype, suppressible by nyo^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

NOT suppressed by

Statement

Reference

tyn^PG38 has lethal phenotype, non-suppressible by m^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has lethal phenotype, non-suppressible by dyl^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has lethal phenotype, non-suppressible by zye^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has lethal phenotype, non-suppressible by m::tyn^PAN-tyn.UAS/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has lethal phenotype, non-suppressible by m::tyn^ZPtyn.UAS/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has lethal phenotype, non-suppressible by m::tyn^ZPm.UAS/Scer\GAL4^da.G32

(Fernandes et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

tyn^PG38 has larval tracheal system | embryonic stage phenotype, suppressible | partially by trk^unspecified

(Wang et al., 2018)

tyn^PG38 has denticle phenotype, suppressible by neo^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has denticle phenotype, suppressible by nyo^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

NOT suppressed by

Statement

Reference

tyn^PG38 has denticle phenotype, non-suppressible by m^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has denticle phenotype, non-suppressible by dyl^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has denticle phenotype, non-suppressible by zye^UAS.cFa/Scer\GAL4^da.G32

(Fernandes et al., 2010)

tyn^PG38 has denticle phenotype, non-suppressible by m::tyn^PAN-tyn.UAS/Scer\GAL4^da.G32

(Fernandes et al., 2010)

Other

Statement

Reference

m¹, tyn^PG38 has denticle phenotype

(Fernandes et al., 2010)

Df(3L)zye^ex72, m¹, tyn^PG38 has denticle phenotype

(Fernandes et al., 2010)

Scer\GAL4^da.G32, m::tyn^ZPtyn.UAS, tyn^PG38 has denticle phenotype

(Fernandes et al., 2010)

Scer\GAL4^da.G32, m::tyn^ZPm.UAS, tyn^PG38 has denticle phenotype

(Fernandes et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

m¹, tyn^PG38 double mutants have denticles with thin extremities (as found in tyn^PG38 single mutants) and lacking the medial constriction (as found in m¹ single mutants). The addition of Df(3L)zye^ex72, which deletes zye, has a further effect, leading to denticles with a twisted appearence.

Expression of neo^Scer\UAS.cFa or nyo^Scer\UAS.cFa using Scer\GAL4^da.G32 partially restores the viability of tyn^PG38 animals, and very partially ameliorates the tyn^PG38 denticle phenotype.

Expression of m::tyn^{ZPtyn.Scer\UAS} or m::tyn^ZPm.Scer\UAS with Scer\GAL4^da.G32 has a weak effect on the tyn^PG38 denticle phenotype but does not restore adult viability.

(Fernandes et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Not rescued by

tyn^PG38 is not rescued by tyn^ΔPAN.UAS/Scer\GAL4^da.G32

(Fernandes et al., 2010)

Comments

Expression of SP71^Scer\UAS.cFa using Scer\GAL4^da.G32 rescues the denticle and adult viability phenotypes of SP71^PG38 animals.

(Fernandes et al., 2010)

Expression of tyn^Scer\UAS.cFa using Scer\GAL4^da.G32 fully rescues the denticle defects of tyn^PG38 embryos and restores adult viability.

Expression of tyn^{ΔPAN.Scer\UAS} using Scer\GAL4^da.G32 leads to only a slight modification of the tyn^PG38 denticle phenotype and no restoration of tyn^PG38 adult viability.

(Fernandes et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Re-mobilisation of the transposon causes the lethality phenotype to be lost.

(Bourbon et al., 2002)

Excision of the P-element restores a wild type phenotype.

(Fernandes et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG17131-ORFA^PG38

CG17131-ORFB^PG38

SP71^PG38

tyn^PG38

(Wang et al., 2018, Fernandes et al., 2010, Fernandes et al., 2010)

Name Synonyms

Secondary FlyBase IDs

FBal0130767
FBal0157143

References (4)

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