Allele Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}

General Information
Symbol	Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}	Species	A. victoria
Name		FlyBase ID	FBal0157337
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Avic\GFP

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Jin et al., 2004)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Avic\GFP^{E.Scer\UAS.cJa} (Jin et al., 2004)
Nature of the lesion	Statement Reference Construct: the sequence (CGG)₉AGG(CGG)₉AGG(CGG)₇₀ has been inserted between the transcriptional and translational start sites of Avic\GFP. (Jin et al., 2004)
Assay mode	In transgenic Drosophila (allele of foreign species in genome of drosophilid) (Jin et al., 2004)
Carried in construct	P{UAS-EGFP.CGG₉₀} (Jin et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type, with Scer\GAL4^dpp.blk1 (Jin et al., 2004) lethal \| larval stage, with Scer\GAL4^Act5C.PI (Jin et al., 2004) lethal, with Scer\GAL4^elav-C155 (Jin et al., 2004)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Jin et al., 2004) ommatidium, with Scer\GAL4^GMR.PF (Jin et al., 2004) pigment cell, with Scer\GAL4^GMR.PF (Jin et al., 2004)
Detailed Description
	Statement Reference Strong expression of Avic\GFP^{Fmr1.CGG90.E.Scer\UAS} driven by Scer\GAL4^GMR.PF, rough eyes, loss of pigmentation, and severe cell death is seen. When Avic\GFP^{Fmr1.CGG90.E.Scer\UAS} is driven by a 'moderate' expressing line of Scer\GAL4^GMR.PF, a mild rough eye phenotype and holes in tangential sections of the eye are seen. This phenotype strengthens with age. This phenotype also strengthens is expression is increased (by raising the temperature at which the flies are cultured), thus this phenotype is a progressive neurodegeneration. Inclusions are seen in the nuclei of mutant eye cells. Strong expression of Avic\GFP^{Fmr1.CGG90.E.Scer\UAS} driven by Scer\GAL4^elav-C155, lethality is seen. With a 'moderate' expression the mutants have a reduced viability. This phenotype also strengthens is expression is increased (by raising the temperature at which the flies are cultured), thus this phenotype is a progressive neurodegeneration. Strong expression of Avic\GFP^{Fmr1.CGG90.E.Scer\UAS} driven by Scer\GAL4^Act5C.PI, late larval lethality is seen. With a 'moderate' expression the mutants a reduced viability and lethality in males is seen. (Jin et al., 2004)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF (Jin et al., 2004) Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by Hsc70-4^{K71S.Scer\UAS}, Scer\GAL4^GMR.PF (Jin et al., 2004) Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF has pigment cell phenotype, enhanceable by Hsc70-4^{K71S.Scer\UAS}, Scer\GAL4^GMR.PF (Jin et al., 2004)
Suppressed by
Statement Reference Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible \| partially by Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF (Jin et al., 2004) Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF has pigment cell phenotype, suppressible \| partially by Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF (Jin et al., 2004) Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF has eye phenotype, suppressible \| partially by Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF (Jin et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Jin et al., 2004, Neuron 39(5): 739--747 RNA-mediated neurodegeneration caused by the Fragile X premutation rCGG repeats in Drosophila. [FBrf0162265]
Review	Orr, 2004, Trends Neurosci. 27(5): 233--234 RNA gains a new function: a mediator of neurodegeneration. [FBrf0175166]

Allele Avic\GFPFmr1.CGG90.E.Scer\UAS

Allele Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}