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General Information
Symbol
Dmel\Akt1UAS.Exel
Species
D. melanogaster
Name
FlyBase ID
FBal0157364
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-AKT, UAS-AKT1, UAS-Akt1.Exel
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Wild type sequences for the short, 60 kD form of Akt1 have been cloned into P{Express-UAS} where their expression is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

Akt1Scer\UAS.Exel rescues some aspects of the Hsap\HTTQ93.ex1p.Scer\UAS Huntington's disease model but not others: the retinal toxicity and locomotor performance defects are significantly ameliorated, but the brain neuronal death and reduced lifespan are unaffected.

Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

There is no increase in the frequency of clone splitting when Akt1Scer\UAS.Exel-expressing clones are generated in wild type tissues compared with wild type clones in wild type tissue.

Expression of Akt1Scer\UAS.Exel in the eye and head under the control of Scer\GAL4GMR.long results in an increase in head size compared with controls.

Expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4Mef2.PR results in an increase in the breadth of larval muscle fibres.

Expression of Akt1Scer\UAS.Exel in the fat body under the control of Scer\GAL4ppl.PP results in an increase in cell and nucleus size.

Expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4ap-md544 causes the wing to bend downward.

Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent the normal shrinkage and disappearance of pupal neuroblasts.

Expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4ple.PF or Scer\GAL4elav.PU leads to an increase in survival duration by about 5-7 days when exposed to 0.5ppm 1-octen-3-ol.

Expression of Akt1Scer\UAS.Exel in the eye, under the control of Scer\GAL4GMR.PF, does not result in a visible phenotype.

Clones expressing Akt1Scer\UAS.Exel under the control of Scer\GAL4Act5C.PU in the third larval instar wing disc and fat body are larger than control clones.

Axon regeneration of class IV da neurons in the ventral nerve cord (after axon severing at the commissure junction at 48 hours after egg laying) is enhanced in larvae expressing Akt1Scer\UAS.Exel under the control of Scer\GAL4ppk.PG compared to wild type controls.

The dendrites of ddaC neurons of larvae expressing Akt1Scer\UAS.Exel under the control of Scer\GAL4ppk.PG show enhanced regeneration (after dendrite severing at 48 hours after egg laying) compared to wild-type controls. No substantial dendrite regeneration is seen in the mutant larvae after dendrite severing at 72 hours after egg laying.

Neuronal expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4elav-C155 increases sensitivity to ethanol sedation.

Neuronal expression of Akt1Scer\UAS.Exel (under the control of Scer\GAL4elav-C155) throughout development (until eclosion of the adult fly, when Scer\GAL80ts.αTub84B is induced) results in increased ethanol sensitivity. The converse experiment, with over-expression in neurons only after eclosion, does not alter ethanol sensitivity.

Expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4fat results in increased lipid levels compared to controls.

Expression of Akt1Scer\UAS.Exel driven by Scer\GAL4ap-md544 in dorsal cells of the wing causes an overgrowth phenotype in which the wing bends downward.

Expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4Bx-MS1096 results in a down-curled wing phenotype in 53.2% of flies.

Ectopic expression of Akt1Scer\UAS.Exel-driven by Scer\GAL4ppk.PG causes a significant increase in dendrite coverage of larval hemisegments.

Overexpression of Akt1Scer\UAS.Exel using Scer\GAL4en-e16E enlarges the posterior wing compartment area and increases cell size.

Expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4GMR.PU has no effect on the external appearance of the eye. The number of photoreceptors per ommatidium is similar to wild type.

Expression of Akt1Scer\UAS.Exel has no effect on lifespan when expressed under the control of any of Scer\GAL4elav-C155, Scer\GAL4repo.PU or Scer\GAL4Eaat1.PR.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of Akt1Scer\UAS.Exel moderately enhances the increased midgut cell size resulting from the expression of Atg9HMS01246 under the control of Scer\GAL4Myo31DF-NP0001.

The reduced body weight of CycGHR7 larvae is fully rescued by ectopic expression of Akt1Scer\UAS.Exel driven by Scer\GAL4Lsp2.PH.

The lipid droplet accumulation in oenocytes of CycGHR7 mutant larvae is highly suppressed by ectopic expression of Akt1Scer\UAS.Exel under the control of the Scer\GAL4Lsp2.PH driver.

Expression of Akt1Scer\UAS.Exel suppresses the reductions in trichome density and overall wing size seen when trblScer\UAS.T:Zzzz\FLAG is expressed under the control of Scer\GAL4e16A.

Expression of trblScer\UAS.T:Zzzz\FLAG suppresses the increase in head size seen when Akt1Scer\UAS.Exel is expressed in the eye and head under the control of Scer\GAL4GMR.long.

Expression of trblScer\UAS.T:Zzzz\FLAG suppresses the increase in fibre breadth seen in larval muscles when Akt1Scer\UAS.Exel is expressed under the control of Scer\GAL4Mef2.PR.

Expression of trblScer\UAS.T:Zzzz\FLAG suppresses the increase in cell and nucleus size seen when Akt1Scer\UAS.Exel is expressed under in the larval fat body the control of Scer\GAL4ppl.PP.

Expression of trblScer\UAS.T:Zzzz\FLAG suppresses the downward wing bending phenotype seen when Akt1Scer\UAS.Exel is expressed under the control of Scer\GAL4ap-md544, resulting in a nearly flat wing blade.

Overexpression of Akt1Scer\UAS.Exel in the salivary glands, under the control of Scer\GAL4ppl.PP, fully suppresses the small cell size phenotype found upon knockdown of CdsA through expression of CdsANIG.7962R. The ectopic lipid accumulation phenotype of CdsANIG.7962R mutants is unaffected by overexpression of Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX but not Akt1Scer\UAS.Exel.

Scer\GAL4GMR.PF-driven expression of Akt1Scer\UAS.Exel enhances the rough eye phenotype of the AcnScer\UAS.cNa transgene.

Scer\GAL4GMR.PF-driven expression of Akt1Scer\UAS.Exel fails to affect the rough eye phenotype of the AcnS641A.S731A.Scer\UAS transgene.

The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv is not suppressed if they are also co-expressing Akt1Scer\UAS.Exel.

Clones expressing PrpkdsRNA.Scer\UAS.462 and also co-expressing Akt1Scer\UAS.Exel under the control of Scer\GAL4Act5C.PU in the third larval instar wing disc and fat body are small and apoptotic, showing the same phenotype as clones expressing PrpkdsRNA.Scer\UAS.462 alone under the control of Scer\GAL4Act5C.PU.

Over-expression of wdbGS9548 in eye imaginal discs (under the control of Scer\GAL4GMR.long) suppresses the increase in eye size caused by overexpression of Akt1Scer\UAS.Exel.

Expression of Akt1Scer\UAS.Exel under the control of Scer\GAL4fat does not rescue lipid levels in Sik348 flies.

Co-expression of Sesnd04539 with Akt1Scer\UAS.Exel via Scer\GAL4ap-md544 suppresses the Akt1Scer\UAS.Exel hyperplastic wing phenotype.

The growth-promoting effect of Akt1Scer\UAS.Exel overexpression via Scer\GAL4ap-md544 is enhanced in a homozygous Sesn8A11-mutant background

Co-expression of GirdindsRNA.Scer\UAS suppresses the elevation of cell size in the posterior of the wing seen when Akt1Scer\UAS.Exel is over-expressed using Scer\GAL4en-e16E.

Xenogenetic Interactions
Statement
Reference

Co-expression of Akt1Scer\UAS.Exel enhances the suppression of the grimGMR.PU eye phenotype seen when Hsap\LRRK2Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PU.

Expression of Akt1Scer\UAS.Exel does not suppress the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1Scer\UAS.cSa is expressed under the control of Scer\GAL4Hml.Δ.

Expression of Akt1Scer\UAS.Exel strongly suppresses the pigment cell degeneration seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

Expression of Akt1Scer\UAS.Exel markedly slows down the progressive photoreceptor degeneration seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Expression of Akt1Scer\UAS.Exel does not suppress the γ-lobe kenyon cell degeneration seen in the mushroom bodies when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Expression of Akt1Scer\UAS.Exel does not suppress the reduction in lifespan seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of any of Scer\GAL4elav-C155, Scer\GAL4repo.PU or Scer\GAL4Eaat1.PR.

Expression of Akt1Scer\UAS.Exel does not suppress the locomotor defects seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155. The locomotor defects seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4repo.PU are partially suppressed.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Akt1Scer\UAS.Exel
Akt1UAS.Exel
Name Synonyms
Secondary FlyBase IDs
    References (30)