Allele Dmel\Adgf-A^{Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\Adgf-A^{Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Dolezal	FlyBase ID	FBal0157456
Feature type	allele	Associated gene	Dmel\Adgf-A

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Dolezal et al., 2005) in vitro construct - regulatory fusion (Dolezal et al., 2003, Dolezal et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a wild-type Adgf-A cDNA which is tagged at the C-terminal end with T:Hsap\MYC. (Dolezal et al., 2005) Construct: Scer\UAS regulatory sequences drive expression of Adgf-A. (Dolezal et al., 2003)
Carried in construct	P{UAS-Adgf-A.myc} (Dolezal et al., 2005, Dolezal et al., 2003)
Tagged with	T:Hsap\MYC (Dolezal et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, Adgf-A^{Scer\UAS.T:Hsap\MYC} rescues Dp(3;3)Gerda (Dolezal et al., 2003) Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, Adgf-A^{Scer\UAS.T:Hsap\MYC} rescues Dp(3;3)Karel (Dolezal et al., 2003) Scer\GAL4^Act.PU/Adgf-A^{Scer\UAS.T:Hsap\MYC} rescues Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005) Scer\GAL4^c564/Adgf-A^{Scer\UAS.T:Hsap\MYC} rescues Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005) Scer\GAL4^e33C/Adgf-A^{Scer\UAS.T:Hsap\MYC} rescues Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005)
Partially rescues	Scer\GAL4⁵⁰¹⁵/Adgf-A^{Scer\UAS.T:Hsap\MYC} partially rescues Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005) Scer\GAL4^Cg.PA/Adgf-A^{Scer\UAS.T:Hsap\MYC} partially rescues Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005) Scer\GAL4^Dot.PK/Adgf-A^{Scer\UAS.T:Hsap\MYC} partially rescues Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005)
Fails to rescue	Scer\GAL4^Lsp2.PH/Adgf-A^{Scer\UAS.T:Hsap\MYC} fails to rescue Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005) Scer\GAL4^Mef2.PR/Adgf-A^{Scer\UAS.T:Hsap\MYC} fails to rescue Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005) Scer\GAL4^T110/Adgf-A^{Scer\UAS.T:Hsap\MYC} fails to rescue Adgf-A^Karel.3'/Adgf-A^Karel.5' (Dolezal et al., 2005)
Comments	Expression of Adgf-A^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act completely rescues the mutant phenotype of Dp(3;3)Karel animals (Dp(3;3)Karel carries the Adgf-A^Karel.3' and Adgf-A^Karel.5' mutant alleles). The lethality and melanotic tumour phenotypes of Dp(3;3)Karel homozygotes are fully rescued by expression of Adgf-A^{Scer\UAS.T:Hsap\MYC} under the control of one of Scer\GAL4^e33C, Scer\GAL4^Cg.PA or Scer\GAL4^c564. Expression of Adgf-A^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^e33C or Scer\GAL4^c564 reduces the number of circulating hemocytes in Dp(3;3)Karel third instar larvae to normal levels. Expression of Adgf-A^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Cg.PA also reduces the number of circulating hemocytes in Dp(3;3)Karel third instar larvae, but to a lesser extent. (Dolezal et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Adgf-A^Scer\UAS.cDa Adgf-A^{Scer\UAS.T:Hsap\MYC}
Name Synonym	Saccharomyces cerevisiae UAS construct a of Dolezal (Dolezal et al., 2005, Dolezal et al., 2003)
Secondary FlyBase IDs

References ( 2 )
Research paper	Dolezal et al., 2005, PLoS Biol. 3(7): e201 A role for adenosine deaminase in Drosophila larval development. [FBrf0188209] Dolezal et al., 2003, Genetics 165(2): 653--666 Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila. [FBrf0167663]

Allele Dmel\Adgf-AScer\UAS.T:Hsap\MYC

Allele Dmel\Adgf-A^{Scer\UAS.T:Hsap\MYC}