Open Close
General Information
Symbol
Dmel\twsUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Bajpai
FlyBase ID
FBal0158539
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Tws
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of full length tws cDNA (LD12394) is under the control of UAS sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of twsScer\UAS.cBa under the control of either Scer\GAL4elav.PU or Scer\GAL4Act5C.PU results in a large number of apoptotic cells in embryos.

Expression of twsScer\UAS.cBa under the control of Scer\GAL4elav.PU reduces the eclosion rate to approximately 40%.

Flies expressing twsScer\UAS.cBa under the control of Scer\GAL4elav.PU at 29[o]C show a severe reduction in lifespan compared to controls.

Newly eclosed adults expressing twsScer\UAS.cBa under the control of Scer\GAL4elav.PU at 29[o]C do not show abnormalities in the brain. However, 7 day old flies show vacuolisation of both cortex and neuropil.

Mitochondrial size is reduced and mitochondrial density is increased in the axons of the larval ventral ganglia in animals expressing twsScer\UAS.cBa under the control of Scer\GAL4en.PU. The mitochondrial reticulum is disintegrated into multiple punctiform organelles.

Mitochondria of photoreceptor neurons are considerably smaller than controls in flies expressing twsScer\UAS.cBa under the control of Scer\GAL4elav.PU. Scission of mitochondria is seen. Internal cristae of disintegrated mitochondria are less condensed than normal and some are circled by autophagosome-like organelles.

Mitochondrial membrane potential is significantly reduced in the heads of flies expressing twsScer\UAS.cBa under the control of Scer\GAL4elav.PU.

Flies expressing twsScer\UAS.cBa under the control of Scer\GAL4elav.PU at 29[o]C and treated with 20mM paraquat show reduced climbing ability compared to control flies.

twsScer\UAS.cBa; Scer\GAL4hs.PB; tws60/tws60 animals have scattered ectopic bristles on their wing blades.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Scer\GAL4elav.PU, twsUAS.cBa has short lived | heat sensitive phenotype, suppressible by Scer\GAL4elav.PU/Sod2UAS.cMa

Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Sod2Scer\UAS.cMa extends the lifespan of flies expressing twsScer\UAS.cBa under the control of Scer\GAL4elav.PU at 29[o]C.

Xenogenetic Interactions
Statement
Reference

Expression of twsScer\UAS.cBa mitigates the Hsap\MAPTScer\UAS.cWa-induced bristle loss phenotype (with both under the control of Scer\GAL4Eq1). Bristle and sensory cell numbers increase concurrently when twsScer\UAS.cBa is co-expressed with Hsap\MAPTScer\UAS.cWa.

Expression of twsScer\UAS.cBa in larval da neurons (under the control of Scer\GAL4ppk.PG) suppresses the aggregate formation and dysmorphology of neurites found upon expression of Hsap\MAPTScer\UAS.cWa.

Complementation and Rescue Data
Fails to rescue
Comments

twsScer\UAS.cBa; Scer\GAL4hs.PB rescues survival of tws60 to the pharate adult stage, with no obvious morphological defects apart from scattered ectopic bristles on the wing blades. The larval/pupal lethality of twsj11C8 homozygotes is not rescued by twsScer\UAS.cBa; Scer\GAL4hs.PB, but is partially rescued by twsScer\UAS.cBa; Scer\GAL4en-e16E.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
twsScer\UAS.cBa
twsUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct of Bajpai
Secondary FlyBase IDs
    References (3)