FB2025_01 , released February 20, 2025
Allele: Dmel\topiZ3-2139
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General Information
Symbol
Dmel\topiZ3-2139
Species
D. melanogaster
Name
FlyBase ID
FBal0158555
Feature type
allele
Associated gene
Dmel\topi
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Perezgasga et al., 2004)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q521term.

(Perezgasga et al., 2004)

Nucleotide substitution: C1584T.

(Perezgasga et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:9,776,966..9,776,966 [+]
Nucleotide change:

C9776966T

Reported nucleotide change:

C1584T

Amino acid change:

Q522term | topi-PA

Reported amino acid change:

Q521term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Perezgasga et al., 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      topiZ3-2139/topiZ3-0707 heterozygote males show morphological defects of the nuclear lamina in stage S4-S6 spermatocytes (the lamina has more ballon-like shape, largely lacking the deformations that correlate with chromosome distribution seen in wild-type), while in stage S3 the lamina is of wild-type appearance.

      (Trost et al., 2016)

      topiZ3-2139/topiZ3-0707 mutant spermatocytes exhibit meiotic arrest and other defects, including bivalent chromosomes condensing to spherical shapes away from the nuclear periphery, and failure of the nucleolus to fully break down.

      (Lu and Fuller, 2015)

      Chromosomes in mutant primary spermatocytes are partially condensed and are not close to the nuclear envelope (in contrast to wild-type primary spermatocytes where they are decondensed and adjacent to the nuclear envelop).

      (Jiang et al., 2007)

      The testes of topiZ3-2139/topiZ3-2139 adult males contain germ cell cysts up to and including mature primary spermatocytes, but lack germ-cells in meiotic division or spermatid differentiation stages. Degenerating primary spermatocytes are present in the distal region of the testis.

      (Perezgasga et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Zuker

      (Perezgasga et al., 2004)

      From C.Zucker's collection of viable, male sterile lines.

      (Perezgasga et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      topiZ2139
      (Lu and Fuller, 2015)
      topiZ3-2139
      (Trost et al., 2016, Doggett et al., 2011, Di Cara et al., 2010, Jiang et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)