Allele Dmel\spas^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\spas^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0158618
Feature type	allele	Associated gene	Dmel\spas

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Trotta et al., 2004) in vitro construct - RNAi (Trotta et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a fusion of spas cDNA and genomic sequences, which are arranged so that they should produce dsRNA. (Trotta et al., 2004)
Carried in construct	P{UAS-spas.RNAi} (Trotta et al., 2004, Jinushi-Nakao et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^αTub84B.PL (Trotta et al., 2004) locomotor behavior defective \| adult stage, with Scer\GAL4^elav-C155 (Trotta et al., 2004) neuroanatomy defective, with Scer\GAL4^elav-C155 (Trotta et al., 2004) neuroanatomy defective, with Scer\GAL4^ppk.PG (Jinushi-Nakao et al., 2007) neurophysiology defective, with Scer\GAL4^elav-C155 (Trotta et al., 2004) uncoordinated \| adult stage, with Scer\GAL4^elav-C155 (Trotta et al., 2004)
Phenotype Manifest In
	class IV dendritic arborizing neuron, with Scer\GAL4^ppk.PG (Jinushi-Nakao et al., 2007) dendrite, with Scer\GAL4^ppk.PG (Jinushi-Nakao et al., 2007) dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.PG (Jinushi-Nakao et al., 2007) neuromuscular junction \| larval stage, with Scer\GAL4^elav-C155 (Trotta et al., 2004)
Detailed Description
	Statement Reference Reduction of spas levels through expression of spas[dsRNA.Scer\UAS] under the control of Scer\GAL4[ppk.PG] leads to large gaps both between neighboring class IV dendritic arbors and within the arbor of an individual neuron. spas knockdown neurons exhibit 17% more uncovered area than wild-type ddaC neurons. Expression of spas[dsRNA.Scer\UAS] under the control of Scer\GAL4[221] has no effect on either branching or dendrite length. (Jinushi-Nakao et al., 2007) Adults expressing spas^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 show defects in coordination and locomotory behaviour. Expression of spas^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in a severe reduction of total synaptic area at the larval neuromuscular junction (NMJ) compared to controls (the reduction in synaptic area at the muscle 6/7 NMJ is about 50%). These animals show a significant increase in mean excitatory junctional current (EJC) amplitude at the larval NMJ compared to controls (138.9 +/- 17.3nA compared to 85.3 +/- 3.8 nA). This increase in mean EJC amplitude can be rescued if the animals are pretreated with 3μM nocodazole before recording the current. (Trotta et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference spas^{dsRNA.Scer\UAS}/Scer\GAL4²²¹ is a suppressor of neuroanatomy defective phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007)
Other
Statement Reference Scer\GAL4²²¹, kn^Scer\UAS.cMa, spas^{dsRNA.Scer\UAS} has neuroanatomy defective phenotype (Jinushi-Nakao et al., 2007)
Phenotype Manifest In
Suppressor of
Statement Reference spas^{dsRNA.Scer\UAS}/Scer\GAL4²²¹ is a suppressor of class I dendritic arborizing neuron phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007) spas^{dsRNA.Scer\UAS}/Scer\GAL4²²¹ is a suppressor of dendrite phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007)
Other
Statement Reference Scer\GAL4²²¹, kn^Scer\UAS.cMa, spas^{dsRNA.Scer\UAS} has class IV dendritic arborizing neuron phenotype (Jinushi-Nakao et al., 2007) Scer\GAL4²²¹, kn^Scer\UAS.cMa, spas^{dsRNA.Scer\UAS} has dendrite phenotype (Jinushi-Nakao et al., 2007) Scer\GAL4²²¹, kn^Scer\UAS.cMa, spas^{dsRNA.Scer\UAS} has dorsal multidendritic neuron ddaC phenotype (Jinushi-Nakao et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Ectopic expression of spas[dsRNA.Scer\UAS] strongly reduces both branching and total dendrite length by 18 and 19% respectively, when expressed in kn[Scer\UAS.cMa]-expressing class I neurons (both transgenes under the control of Scer\GAL4[221]). (Jinushi-Nakao et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Used to study the phenotypic consequences of dsRNA interference (RNAi) of the spas gene. (Trotta et al., 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	spas^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Jinushi-Nakao et al., 2007, Neuron 56(6): 963--978 Knot/Collier and cut control different aspects of dendrite cytoskeleton and synergize to define final arbor shape. [FBrf0202055] Trotta et al., 2004, Curr. Biol. 14(13): 1135--1147 The hereditary spastic paraplegia gene, spastin, regulates microtubule stability to modulate synaptic structure and function. [FBrf0179488]

Allele Dmel\spasdsRNA.Scer\UAS

Allele Dmel\spas^{dsRNA.Scer\UAS}