|Feature type||allele||Associated gene||Dmel\spas|
|Mutagen||in vitro construct - regulatory fusion, in vitro construct - RNAi|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Reduction of spas levels through expression of spas[dsRNA.Scer\UAS] under the control of Scer\GAL4[ppk.PG] leads to large gaps both between neighboring class IV dendritic arbors and within the arbor of an individual neuron. spas knockdown neurons exhibit 17% more uncovered area than wild-type ddaC neurons. Expression of spas[dsRNA.Scer\UAS] under the control of Scer\GAL4 has no effect on either branching or dendrite length.
Adults expressing spasdsRNA.Scer\UAS under the control of Scer\GAL4elav-C155 show defects in coordination and locomotory behaviour. Expression of spasdsRNA.Scer\UAS under the control of Scer\GAL4elav-C155 results in a severe reduction of total synaptic area at the larval neuromuscular junction (NMJ) compared to controls (the reduction in synaptic area at the muscle 6/7 NMJ is about 50%). These animals show a significant increase in mean excitatory junctional current (EJC) amplitude at the larval NMJ compared to controls (138.9 +/- 17.3nA compared to 85.3 +/- 3.8 nA). This increase in mean EJC amplitude can be rescued if the animals are pretreated with 3μM nocodazole before recording the current.
|Phenotype Manifest In|
spasdsRNA.Scer\UAS/Scer\GAL4221 is a suppressor of class I dendritic arborizing neuron phenotype of Scer\GAL4221, knScer\UAS.cMa
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 2 )|